Studies on the Digestive Enzymes of Rainbow Trout-Ii

Kitamikado, Manabu; TACHINO, Shinko

doi:10.2331/suisan.26.685

Cited by 43 publications

(15 citation statements)

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“…Ca 2+ ions (0.5 mM CaCl 2 ) stabilized the activity of the trypsins obtained, especially at acidic pH values. Similar properties of proteinases were described for other fish species [24][25][26]. Mg 2+ and Mn 2+ ions (5 mM MgCl 2 and 5 mM MnCl 2 ) had different effects on the activities of isolated elastases during a 30-min incubation at 35°C and pH 8.0.…”

Section: Resultssupporting

confidence: 63%

Purification and properties of serine proteinases from European catfish Silurus glanis L. pancreas

Ulitina

Khablyuk

Proskuryakov

2005

Appl Biochem Microbiol

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Three trypsin isoforms (designated as T1, T2, and T3), three chymotrypsin isoforms (Kh1, Kh2, and Kh3), and two elastase isoforms (E1 and E2) were isolated from the pancreas of European catfish Silurus glanis L. by salting out with (NH 4 ) 2 SO 4 , gel chromatography on Sephadex G-75, and ion exchange chromatography on DEAE cellulose. Isoelectric points of the enzymes, determined by isoelectric focusing, amounted to 4.42 for T1, 5.64 for T2, 6.90 for T3, 4.93 for Kh1, 5.23 for Kh2, 6.18 for Kh3, 6.17 for E1, and 8.48 for E2. Molecular weights of proteinases within each group were close and amounted to 30,100 Da for trypsins, 39,800 Da for chymotrypsins, and 24,000 Da for elastases. The enzymes isolated displayed maximal activities at alkaline pH values. Inhibitor analysis demonstrated that all the proteinases isolated from European catfish pancreas belonged to the serine type.

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Section: Resultssupporting

confidence: 63%

Purification and properties of serine proteinases from European catfish Silurus glanis L. pancreas

Ulitina

Khablyuk

Proskuryakov

2005

Appl Biochem Microbiol

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“…Differences in preparation of raw extracts resulted in significant differences between our results and those obtained by Siebert & Bottke (1963 the organs of rainbow trout reach a proteolytic activity which remains constant up to a body weight of 70 g . Also Kitamikado & Tachino (1960) found that between 12 and 980 g of body weight, proteolytic activity of the stomach (pH 2 .8) does not change. Hence, it can be supposed that values of the proteolytic activity of the organs, as obtained in our studies, are correct in a broader range of body weight of the fish .…”

Section: Resultsmentioning

confidence: 98%

“…Proteolytic enzymes take part in the utilization of proteins, and consequently, they directly affect the growth of an organism . Properties of these enzymes are relatively best known in carp and trout (Bondi & Spandorf, 1954 ;Kitamikado & Tachino, 1960 ;Siebert & Bottke, 1963 ;Chepik, 1964a, b;Onishi, Murayama & Takeuchi, 1973a, b ;Trofimova, 1973) . Activity of the enzymes was observed in the youngest developmental stages (Kawai & Ikeda, 1973a, b) .…”

Section: Introductionmentioning

confidence: 99%

Studies on the role of exogenous proteolytic enzymes in digestion processes in fish

Dąbrowski¹,

Głogowski²

1977

Hydrobiologia

136

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Studies were carried out on the proteolytic activity of the fry of common carp, rainbow trout, grass carp, and whitefish, as well as on the activity of digestive organs of adult common carp and rainbow trout . Activity of exogenous enzymes in relation to endogenous ones was assessed on the basis of the proteolytic value of fish food and the activity of digestive organs . It was found that the share of proteolytic enzymes of natural food in the digestion process in fish was high . Beginning from a weight of 50-100 g for common carp and 1o g for rainbow trout, the relation between the daily enzymatic ration and the weight of fish indicates the cooperation of an approximately constant amount of exogenous enzymes .

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“…Alternatively, differential ability to digest proteins between size classes of abalone and, therefore, the availability of protein to the abalone cannot be discounted. Studies with some fish species (Kitamikado & Tachino 1960; Rajamani & Job 1976; Ferraris, Catacutan, Mabelin & Jazul 1986) have demonstrated that digestibility increases with animal size, and while there are currently no extensive studies into the effects of animal size on protein digestibility in abalone, Uki & Wantanabe (1992) reported differences in the ability of two size classes of H. discus hannai to digest white fishmeal. However, as it was found that digestibility decreased with increasing animal size, protein availability would be expected to be greatest in the smaller abalone.…”

Section: Discussionmentioning

confidence: 99%

The effect of animal size on the ability of Haliotis midae L. to utilize selected dietary protein sources

Shipton

Britz

2001

Aquaculture Research

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The effect of animal size on the qualitative protein requirements of two size classes of Haliotis midae L. was assessed by feeding 12 semi‐purified single protein test diets (20% protein, 6% lipid) to juvenile and young adult animals (10–20‐ and 40–50‐mm initial shell length). The protein sources selected for the trial comprised four fishmeals, casein, spirulina, abalone viscera silage, brewery waste, torula yeast, carcass, sunflower and cotton seed meals. The results indicated that in terms of growth and feed efficiency, the fishmeals and spirulina were the most suitable candidates for use as primary protein sources in formulated feeds, and with the exceptions of the carcass meal and brewery waste, the remaining protein sources demonstrated promise as partial primary protein source replacements. Mean growth rates for the large and small abalone over the experimental period were 1.45 and 1.24 mm month−1 respectively. With respect to the larger size class of abalone, the smaller abalone displayed significantly reduced growth (F = 64.7, P < 0.0001), feed conversion ratio (F = 16.6, P < 0.0001) and protein efficiency (F = 26.8, P < 0.0001). Two‐way analysis of variance revealed significant interactions between protein source, animal size and feed conversion ratio (F = 2.4, P < 0.01) and growth (F = 5.4, P < 0.05), thus indicating that qualitative differences exist between the dietary protein requirements of the juvenile and young adult abalone.

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Studies on the Digestive Enzymes of Rainbow Trout-Ii

Cited by 43 publications

References 0 publications

Purification and properties of serine proteinases from European catfish Silurus glanis L. pancreas

Purification and properties of serine proteinases from European catfish Silurus glanis L. pancreas

Studies on the role of exogenous proteolytic enzymes in digestion processes in fish

The effect of animal size on the ability of Haliotis midae L. to utilize selected dietary protein sources

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