Studies on the Detection, Transmission and Distribution of Peach Latent Mosaic Viroid in Peach Trees

Flores, Ricardo; Hernández, Carmen; Avinent, L.; Hermoso, A.; Llácer, G.; Juárez, José; Arregui, J. M.; Navarro, Luis; Desvignes, J.C.

doi:10.17660/actahortic.1992.309.47

Cited by 25 publications

(22 citation statements)

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“…Initial electrophoretic analyses indicated the presence of HSVd among apricot and peach orchard trees from the regions of Valencia and Murcia (Flores et al, 1990;Flores et al, 1992;Marcos and Pall~is, unpublished), As a first step to demonstrate and further evaluate the occurrence of HSVd, we decided to set up a non-radioactive hybridization procedure for the sensitive detection of this viroid. A nearly full-length cDNA corresponding to a Spanish peach HSVd isolate was cloned, as described above, under the control of two different phage transcriptional promoters to yield pHSVd.EB (Figure 1 a).…”

Section: Development Of a Non-radioactive Rna Probe To Diagnose Hsvdmentioning

confidence: 99%

Studies on the diagnosis of hop stunt viroid in fruit trees: Identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents

et al. 1996

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A non-radioactive digoxigenin-labelled RNA probe specific for hop stunt viroid (HSVd) diagnosis has been developed. The high sensitivity and specificity of this RNA probe in dot blot hybridizations to nucleic acids from field samples, allowed the confirmation of the presence of HSVd in apricot (Prunus armeniaca L.) and its detection in two fruit tree species not previously described as hosts of this pathogen, almond (Prunus dulcis Miller) and pomegranate (Punica granatum L.). This result supports and extends the notion of the world wide distribution of HSVd, infecting cultivated fruit trees. HSVd was also found to accumulate to much higher levels in mature apricot fruits than in leaves. Additionally, a sample processing procedure which does not involve the use of organic solvents was demonstrated to render faithful results when used for viroid detection. The combined reliability and facility of use of both this extraction procedure and the non-radioactive probe will benefit agronomic investigations addressing the detection and eradication of HSVd. Other applications of the work described here, as the study of possible causal relations between specific disorders and HSVd infection, are also discussed.

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Section: Development Of a Non-radioactive Rna Probe To Diagnose Hsvdmentioning

confidence: 99%

Studies on the diagnosis of hop stunt viroid in fruit trees: Identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents

et al. 1996

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“…PLMVd is well distributed in shoots, leaves, bark, roots and fruits. Young expanding fruits seem to be the best source of PLMVd (Flores et al 1992;Delgado et al 2005). The molecular mechanisms through which viroids may induce their characteristic effects (symptoms) are not clearly understood.…”

Section: Symptom Expressionmentioning

confidence: 99%

Molecular Biology in Plant Pathogenesis and Disease Management

2008

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“…In peach ( Prunus persicae ), PLMVd can induce a broad variety of symptoms: mosaic, large white patches covering most of the leaf blade, irregularly shaped colourless fruits with cracked sutures and enlarged pits, bud necrosis and delay in foliation, flowering and maturity and a general wilting (Desvignes, 1986). The incidence of PLMVd reached up to 60% in North American germplasm (Shamloul et al., 1995; Hadidi et al., 1997), 40% in Syrian germplasm (Ismaeil et al., 2001), 50% in Italian germplasm (Loreti et al., 1998), 64% in Chinese germplasm (Zhang et al., 2000), 68% in Australian germplasm (Di Serio et al., 1999), 80–85% in Spanish germplasm (Flores et al., 1992) and 90% in Japanese germplasm (Osaki et al., 1999). In orchards, the infection rate of commercial varieties of peach and nectarine in five USA states reached 50% (Skrzeczkowski et al., 1996).…”

Section: Introductionmentioning

confidence: 99%

Distribution of Peach latent mosaic viroid in Commercial Orchards of Peach in the North of Tunisia

Hassen¹,

Massart²,

Roussel³

et al. 2007

Journal of Phytopathology

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The presence of Peach latent mosaic viroid (PLMVd) was monitored during 2 years in peach orchards located in the North of Tunisia. PLMVd population was surveyed using a specific RT-PCR test adapted to crude sap extract. During the first year (2004), 228 samples were randomly selected in 22 peach orchards (eight cultivars) and tested for the presence of PLMVd. Results showed that PLMVd is highly and equally present in four regions of the North of Tunisia. Analyses of some key factors in relation to PLMVd incidence revealed that the tree age did not influence the infection rate. The eight studied cultivars were clustered in three groups according to their PLMVd incidence. Furthermore, the early and season cultivars were statistically more infected than the late cultivars. Prospections in May and October 2005 were performed in four selected orchards. Each two orchards contained Early May Crest (early cultivar) and Carnival (late cultivar) cultivars, respectively. The difference in PLMVd incidence observed in 2004 was confirmed. Furthermore, no correlation between the tree physiological state and the viroid detection was observed. Further investigations on the origin of the differences in PLMVd incidence between Early May Crest and Carnival cultivars would be of interest.

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Studies on the Detection, Transmission and Distribution of Peach Latent Mosaic Viroid in Peach Trees

Cited by 25 publications

References 0 publications

Studies on the diagnosis of hop stunt viroid in fruit trees: Identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents

Studies on the diagnosis of hop stunt viroid in fruit trees: Identification of new hosts and application of a nucleic acid extraction procedure based on non-organic solvents

Molecular Biology in Plant Pathogenesis and Disease Management

Distribution of Peach latent mosaic viroid in Commercial Orchards of Peach in the North of Tunisia

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