Studies on the composition and structure of plasma lipoproteins

Isolation and partial characterization of apolipoprotein D: A new protein moiety of the human plasma lipoprotein system

“…Double-diffusion and immunoelectrophoresis in 1% agarose gels were carried out according to previously described procedures [9]. Immunoprecipitin lines were stained with Amido black 10B and Oil red O.…”

“…Immunoprecipitin lines were stained with Amido black 10B and Oil red O. The preparation and characterization of monospecific antisera to human A-I, A-II, C-I, C-II, and C-Ill polypeptides and LP-B have already been described [9]. Rabbit antisera to human ct-LP and albumin were purchased from Behringwerke A.G., Marburg an der Lahn, GFR.…”

“…The commercial antiserum to a-LP gave two precipitin lines with HDL 3 when tested by double diffusion analysis. One line corresponded to the 'thinline' polypeptide and the other to LP-A [6,9,11].…”

“…Basic 7% polyacrylamide gel electrophoresis has been described elsewhere [9]. Amino acid analyses were performed on a Beckman Model 120C amino acid analyzer by an accelerated automatic procedure on spherical resins [11 ].…”

“…A-II, C-I, C-II, C-Ill and LP-B* [4][5][6]. An additional antigenic component detected predominantly in HDL [7][8][9][10] has been designated 'thin-line' polypeptide due to its characteristic appearance on double diffusion analyses of HDL with anti-HDL or anti-whole serum. Under such experimental conditions, the 'thinline' polypeptide appears as a characteristic thin precipitin line nearer the antigen well, while the major precipitin band due to LP-A is closer to the antibody well [9,11].…”