Studies on the character and staining of fibrin

Lendrum, A. C.; Fraser, D. S.; Slidders, W.; Henderson, Robin A.

doi:10.1136/jcp.15.5.401

Cited by 401 publications

(167 citation statements)

References 11 publications

Supporting

Mentioning

166

Contrasting

Unclassified

Order By: Relevance

“…Prussian blue reaction was used to show iron deposition within the MCA and the peri-arterial tissue. Fibrin stain was performed to examine the thrombus according to the method by Lendrum et al (1962). For this purpose, sections were first deparaffinized.…”

Section: Histologic Demonstration Of Thrombus Formationmentioning

confidence: 99%

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Karataş

Erdener

Gürsoy‐Özdemir

et al. 2011

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

Intravital or multiphoton microscopy and laser-speckle imaging have become popular because they allow live monitoring of several processes during cerebral ischemia. Available rodent models have limitations for these experiments; e.g., filament occlusion of the proximal middle cerebral artery (MCA) is difficult to perform under a microscope, whereas distal occlusion methods may damage the MCA and the peri-arterial cortex. We found that placement of a 10% FeCl 3 -soaked filter paper strip (0.3¾1 mm 2 ) on the duramater over the trunk of the distal MCA through a cranial window for 3 minutes induced intraarterial thrombus without damaging the peri-arterial cortex in the mouse. This caused a rapid regional cerebral blood flow decrease within 10 minutes and total occlusion of the MCA segment under the filter paper in 17±2 minutes, which resulted in a typical cortical infarct of 27 ± 4 mm 3 at 24 hours and moderate sensorimotor deficits. There was no significant hemispheric swelling or hemorrhage or mortality at 24 hours. Reperfusion was obtained in half of the mice with tissue plasminogen activator, which allowed live monitoring of clot lysis along with restoration of tissue perfusion and MCA flow. In conclusion, this relatively simple and noninvasive stroke model is easy to perform under a microscope, making it suitable for live imaging and thrombolysis studies.

show abstract

Section: Histologic Demonstration Of Thrombus Formationmentioning

confidence: 99%

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Karataş

Erdener

Gürsoy‐Özdemir

et al. 2011

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

show abstract

“…Sections were dewaxed and hydrated with distilled water in preparation for a modified version of the MSB method for staining fibrin, collagen, and erythrocytes. 24 Alternating sections were stained with H&E.…”

Section: Histologic Assessmentmentioning

confidence: 99%

Mechanical Characterization of Thromboemboli in Acute Ischemic Stroke and Laboratory Embolus Analogs

Chueh¹,

Wakhloo²,

Hendricks³

et al. 2011

AJNR Am J Neuroradiol

164

130

View full text Add to dashboard Cite

show abstract

“…Formalin-fixed sections were stained for H&E or Martius yellow-brilliant crystal scarlet-soluble blue (MSB, a histological stain for fibrin) (Lendrum et al, 1962). Using consecutive microscopic fields, tumour necrosis was assessed semi-quantitatively using a Chalkley grid method (% necrosis).…”

Section: In Vivo Studymentioning

confidence: 99%

Fibrinogen E fragment selectively disrupts the vasculature and inhibits the growth of tumours in a syngeneic murine model

et al. 2002

View full text Add to dashboard Cite

We recently demonstrated that a fragment of human fibrinogen, fibrinogen E fragment, inhibits the migration and differentiation of human endothelial cells in vitro. Here we show that it exerts similar effects on murine endothelial cells in vitro, and selectively disrupts tumour endothelium in vivo, causing widespread intravascular thrombosis and retarding the growth of CT26 tumours in a syngeneic murine model.

show abstract

Studies on the character and staining of fibrin

Cited by 401 publications

References 11 publications

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Mechanical Characterization of Thromboemboli in Acute Ischemic Stroke and Laboratory Embolus Analogs

Fibrinogen E fragment selectively disrupts the vasculature and inhibits the growth of tumours in a syngeneic murine model

Contact Info

Product

Resources

About

Studies on the character and staining of fibrin

Cited by 401 publications

References 11 publications

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl3 application: A novel model suitable for intravital microscopy and thrombolysis studies

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl3 application: A novel model suitable for intravital microscopy and thrombolysis studies

Mechanical Characterization of Thromboemboli in Acute Ischemic Stroke and Laboratory Embolus Analogs

Fibrinogen E fragment selectively disrupts the vasculature and inhibits the growth of tumours in a syngeneic murine model

Contact Info

Product

Resources

About

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies

Thrombotic distal middle cerebral artery occlusion produced by topical FeCl₃ application: A novel model suitable for intravital microscopy and thrombolysis studies