Studies on the biosynthesis of cholesterol

Schwenk, Erwin; Baker, Cyril F.

doi:10.1016/s0003-9861(53)80011-1

Cited by 11 publications

(1 citation statement)

References 3 publications

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“…Measurement in vitro (Dietschy and Siperstein, 1967 ;Dietschy and Wilson, 1968 ;Srere et al, 1950) probably led to erroneous results since naturally occurring regulatory mechanisms were suppressed in those conditions (Chevallier and Math6, 1976). On the other hand, during experiments in vivo the measurement of labeled precursor incorporation into sterols (Jansen et al,1970 ;Popjak and Beeckmans, 1950 ;Schwenk et at., 1955 ;Van Bruggen et al, 1953), without estimating the contribution of cholesterol transfer between the plasma and the organs to the labeling, may have also resulted in some cases in erroneous values. In these conditions, the liver was generally believed to be the major source of cholesterol biosynthesis (Kritchevsky, 1958).…”

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Measurement of the rate of cholesterol synthesis in various organs of the rat in vivo

Magot¹,

Chevallier²

1979

Ann. Biol. anim. Bioch. Biophys.

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Summary. A method to measure the absolute values of the rate of cholesterol synthesis in vivo in the organs of the rat has been described. Adult male rats were intravenously infused for 3.5, 6 and 10 h with sodium 1-14 (C)-acetate and the specific activity of expired C0 2 was recorded during the experiment. Sterol radioactivities were measured in plasma, lipoproteins and in various organs, the radioactivities in each organ being corrected for cholesterol transfer. The rate of cholesterol synthesis in the intact rat and in each organ was calculated from the radioactivities of the sterols synthesized, that of the acetyl CoA metabolized and the rate of acetyl CoA utilization. The validity of the calculation has been discussed. The results showed that the intestine was the major site of cholesterol biosynthesis (about 50 p. 100).

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confidence: 99%