Studies on the biosynthesis of insulin I. The paper chromatographic isolation of 14C-labeled insulin from calf pancreas slices

A Cytochemical Study on the Pancreas of the Guinea Pig

“…Incorporation of radioactive amino acids in vivo has been repeatedly used to demonstrate the synthesis of a number of specific proteins such as muscle proteins (36) and lung antibodies (37) isolated from tissue extracts, as well as radioactive RNase (30,38), trypsinogen, and c~-chymotrypsinogen (28,30) obtained from pancreatic extracts (38) and juice (28,30). Pancreatic slices labeled in vitro have yielded radioactive amylase (39,40), RNase (41), and insulin (41,42). Under similar conditions, radioactive albumin was obtained from liver slices (43)(44)(45)(46).…”

The incorporation of tritium-labelled amino acids into insulins in rat pancreas in vitro

“…5, and Biochemi8try Department, The Wellcome Research Laboratorie, Beckenham, Kent (Received 19 September 1963) In most recent methods for the small-scale purification of insulin from pancreas, an initial extraction with an acidified organic solvent is followed by further purification by chromatographic methods. Such separations may be carried out on columns, with a variety of supporting media (Porter, 1953;Mendiola & Cole, 1960;Davoren, 1962;Epstein & Anfinsen, 1963;Smith, 1964), or alternatively by paper chromatography (Light & Simpson, 1956;Grodsky & Tarver, 1956;Taylor, Humbel, Steinke & Renold, 1961). Paper-chromatographic methods offer the advantage of relative simplicity, and an account is given below of attempts to apply a paper-chromatographic technique to crude extracts of rat pancreas.…”

“…It has been widely recognized that a system in which isolated pancreas is incubated in vitro with labelled substances might serve as a useful model in which to study the biosynthesis of insulin, and slices of ox or calf pancreas (Pettinga & Rice, 1952;Vaughan & Anfinsen, 1954;Light & Simpson, 1956), or fish islet tissue (Humbel, Renold, Herrera & Taylor, 1961), have been used. Incorporation of amino acids into insulin in rat pancreas, however, has not so far been studied in vitro.…”

Studies on Insulin Biosynthesis

“…At present, little is known about the production of insulin in islet tissue. Light and Simpson (13) and others (22)(23)(24) have demonstrated amino acid incorporation into insulin by mammalian pancreas slices. Wagle has presented evidence for insulin synthesis in a cell-free system derived from dog pancreas (25).…”