Studies on the biomechanical properties of maturing reticulocytes

Xie, Lide; Jiang, Yuhui; Yao, Weijuan; Gu, Leyi; Sun, Dawei; Ka, Weibo; Wen, Zhiyuan; Chien, Shu

doi:10.1016/j.jbiomech.2004.11.032

Cited by 16 publications

(8 citation statements)

References 11 publications

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“…Normal blood cells contain about 1% of reticulocytes. However, in pathological conditions such as hemolytic and blood-loss anemia, the number of reticulocytes is increased as a response of bone marrow to anemia [44]. We speculate that the protocol of mercury exposure used in this study did not interfere with bone marrow cellularity, since the increase of reticulocyte levels was observed in peripheral blood ( Figure 6C, white arrow).…”

Section: Discussionmentioning

confidence: 70%

Diphenyl diselenide protects against hematological and immunological alterations induced by mercury in mice

Brandão

Borges

Oliveira

et al. 2008

J Biochem & Molecular Tox

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Mercury is a heavy metal that can cause a variety of toxic effects on the organism, such as hematological and immunological alterations. In the present investigation, deleterious effects of mercury-intoxication in mice and a possible protective effect of diphenyl diselenide (PhSe)(2) were studied. Male adult Swiss albino mice received daily a pretreatment with (PhSe)(2) (15.6 mg/kg, orally) for 1 week. After this week, mice received daily mercuric chloride (1 mg/kg, subcutaneously) for 2 weeks. A number of hematological (erythrocytes, leukocytes, platelets, hemoglobin, hematocrit, reticulocytes, and leukocytes differential) and immunological (immunoglobulin G and M plasma concentration) parameters were evaluated. Another biomarker of tissue damage, lactate dehydrogenase (LDH), was also determined. The results demonstrated that mercury exposure caused a reduction in the erythrocyte, hematocrit, hemoglobin, leukocyte, and platelet counts and an increase in the reticulocyte percentages. (PhSe)(2) was effective in protecting against the reduction in hematocrit, hemoglobin, and leukocyte levels. (PhSe)(2) ameliorated reticulocyte percentages increased by mercury. However, (PhSe)(2) was partially effective in preventing against the decrease in erythrocyte and platelet counts. Immunoglobulin G and M concentrations and LDH activity were increased by mercury exposure, and (PhSe)(2) was effective in protecting against these effects. In conclusion, (PhSe)(2) was effective in protecting against hematological and immunological alterations induced by mercury in mice.

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Section: Discussionmentioning

confidence: 70%

Diphenyl diselenide protects against hematological and immunological alterations induced by mercury in mice

Brandão

Borges

Oliveira

et al. 2008

J Biochem & Molecular Tox

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“…Reticulocytes take a substantially longer time to enter a single pore 30 , and much higher pressure is necessary to bend the reticulocyte membrane and to force it to enter a pipette 28 . The membrane shear elastic modulus of reticulocytes is almost double that of mature RBCs 31 . In our experiments, reticulocytes exhibited velocity on average 67% that of mature RBCs in the diverging geometry, and 61% that of mature RBCs in the converging geometry (Figure 5).…”

Section: Resultsmentioning

confidence: 96%

A microfabricated deformability-based flow cytometer with application to malaria

et al. 2011

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Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated “deformability cytometer” that measures dynamic mechanical responses of 103–104 individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner.

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“…29 Reticulocytes have lower deformability and lower membrane fluidity than mature red blood cells and these indexes normalize during maturation. 30 However, decreased erythrocyte deformabil-767 Coronary Artery Disease/Hemorheologic Variables and High ADP Platelet Reactivity ity and lower hematocrit values resulted in an independent association with high platelet reactivity, suggesting their independent role in modulating responsiveness to antiplatelet drugs.…”

Section: Discussionmentioning

confidence: 99%

Effect of Blood Hematocrit and Erythrocyte Deformability on Adenosine 5′-Diphosphate Platelet Reactivity in Patients With Acute Coronary Syndromes on Dual Antiplatelet Therapy

Cecchi

Marcucci

Paniccia

et al. 2009

The American Journal of Cardiology

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Previous studies have explored the association between hemorheologic alterations and aspirin resistance, pointing out the possible interaction between hematologic components and platelet responsiveness to antiplatelet drugs. The aim of this study was to evaluate the association between hemorheologic variables and residual platelet reactivity in patients with acute coronary syndromes (ACSs) who underwent percutaneous coronary intervention on dual antiplatelet therapy. The study population included 528 patients with ACSs. Hemorheologic studies were performed by assessing whole blood viscosity at 0.512 and 94.5/ second, plasma viscosity, and erythrocyte deformability index. Post-treatment platelet reactivity was investigated by measuring platelet aggregation by adenosine 5=-diphosphate (ADP) 10 mol and a value >70% was defined as high ADP platelet reactivity. Significantly (p <0.01) lower values of hematocrit and erythrocyte deformability and higher values of whole blood viscosity at 94.5/second were found in patients with high ADP platelet reactivity. At multivariate analysis, lower values of hematocrit and erythrocyte deformability index and higher values of whole blood viscosity at 94.5/second and leukocytes (highest vs lowest tertile) also resulted in an independent association with high platelet reactivity, except for leukocytes, after simultaneous adjustment for hematocrit, leukocyte count, and erythrocyte deformability index. In conclusion, these results demonstrate the influence of hematocrit and of erythrocyte deformability on ADP platelet reactivity. These variables could be considered to optimize treatment with antiplatelet therapy in these patients. Use of clopidogrel in addition to acetylsalicylic acid has been demonstrated to be associated with a decrease of cardiovascular events in patients with acute coronary syndromes (ACSs) independently of stent implantation.1,2 Retrospective studies have shown that discontinuation of clopidogrel, even Ն6 months after stent implantation, is associated with an increased risk of thrombotic events in patients with drug-eluting stents.3,4 However, interindividual variabilities in response to clopidogrel and acetylsalicylic acid have been reported in patients with ACS.5 From these assumptions, the phenomenon of clopidogrel resistance has progressively gained great interest 6,7 and recent studies have shown that decreased responsiveness to clopidogrel is associated with an increased incidence of death and cardiovascular events 8 and is a strong predictor of stent thrombosis after drug-eluting stent implantation.9 Previous studies have reported an association between hemorheologic variables and aspirin resistance.10,11 The aim of this study was to evaluate the possible association between hemorheologic variables and residual platelet reactivity to adenosine 5=-diphosphate (ADP) in patients with ACS on dual antiplatelet therapy. MethodsThe study population included 528 consecutive patients with a diagnosis of ACS, 272 with ST-segment elevation myocardial infarction (STEM...

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Studies on the biomechanical properties of maturing reticulocytes

Cited by 16 publications

References 11 publications

Diphenyl diselenide protects against hematological and immunological alterations induced by mercury in mice

Diphenyl diselenide protects against hematological and immunological alterations induced by mercury in mice

A microfabricated deformability-based flow cytometer with application to malaria

Effect of Blood Hematocrit and Erythrocyte Deformability on Adenosine 5′-Diphosphate Platelet Reactivity in Patients With Acute Coronary Syndromes on Dual Antiplatelet Therapy

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