Studies on the binding of fulvic acid with transferrin by spectroscopic analysis

Zhang, Xiufeng; Yang, Guang; Dong, Yu; Zhao, Yong Qing; Sun, Xin; Chen, Lei; Chen, Hongbo

doi:10.1016/j.saa.2014.09.010

Cited by 21 publications

(18 citation statements)

References 32 publications

(33 reference statements)

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“…According to the previously described methodology (Zhang et al, 2015), fluorescence quenching spectra were obtained in a 1-cm path length quartz cell using an excitation wavelength of 225 nm and an emission wavelength of 280-380 nm. Slit widths (10 nm) and scan speed (12 000 nm/min) were kept constant within each data set.…”

Section: Interaction Of Tf With Hp--cdmentioning

confidence: 99%

“…Trp, Tyr and phenylalanine) (Zhang et al, 2015). The fluorescent spectrum of Tf can be influenced by the changes in the micro-environment of these amino acid residues.…”

Section: Interaction Of Tf With Hp--cdmentioning

confidence: 99%

“…It was postulated that the Tf fluorescence quenching was a static quenching process (Zhang et al, 2015). The association constants (Ka) can be calculated from the regression curve based on the following equation (Lehrer, 1971)…”

Section: Interaction Of Tf With Hp--cdmentioning

confidence: 99%

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Delivery of fluorescent-labeled cyclodextrin by liposomes: role of transferrin modification and phosphatidylcholine composition

Chen

Yao

et al. 2016

Journal of Liposome Research

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Drug-in-CD-in-liposome (DCL) systems which encapsulate the drug/CD inclusion complexes into inner aqueous phase of liposomes have been applied as a novel strategy to improve efficacy of lipophilic antitumor drugs. The aim of this work was to assess the role of transferrin (Tf) modification and phosphatidylcholine (PC) composition on the properties of liposomes containing hydroxypropyl-β-cyclodextrin (HP-β-CD). Fluorescence dye, FITC, was conjugated with HP-β-CD to facilitate the analysis. The resulting FITC-HP-β-CD was further encapsulated into liposomes and then the liposomes were modified with Tf. The FITC-HP-β-CD-loaded liposomes with different PC compositions were compared in terms of particle size, zeta potential, FITC content, FITC-HP-β-CD leakage, phase transition temperature (T) and cellular uptake. The apparent partition coefficient values of different PCs were also determined. Compared to PEGylated liposomes, FITC-HP-β-CD-loaded liposomes modified with Tf had been proved to significantly increase vesicle stability and specific cellular uptake. Moreover, PC composition affected the properties of liposomes. Soybean phosphatidylcholine (SPC) liposomes modified with Tf were found to be more easily internalized into tumor cells than 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and hydrogenated soybean phosphatidylcholine (HSPC) while Tf density on the liposomal surface was similar. And the lipophilicity of SPC was found to be much higher than DPPC and HSPC. Collectively, by the optimization of PC composition, the development of DCL modified with Tf might represent a potential strategy for the antitumor application of lipophilic drugs.

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Section: Interaction Of Tf With Hp--cdmentioning

confidence: 99%

“…Trp, Tyr and phenylalanine) (Zhang et al, 2015). The fluorescent spectrum of Tf can be influenced by the changes in the micro-environment of these amino acid residues.…”

Section: Interaction Of Tf With Hp--cdmentioning

confidence: 99%

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Delivery of fluorescent-labeled cyclodextrin by liposomes: role of transferrin modification and phosphatidylcholine composition

Chen

Yao

et al. 2016

Journal of Liposome Research

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“…In this method, the molecular side chains of lignite are broken to form small molecular compounds under the oxidative degradation of hydrogen peroxide. FA obtained from lignite has attracted increasing attention because of its valuable applications in many fields, such as environmental restoration, agriculture, medicine and aquaculture . Compared with humic acid, FA has strong metal ion complex adsorption characteristics .…”

Section: Introductionmentioning

confidence: 99%

Extraction and Functional Group Characterization of Fulvic Acid from Hami Lignite

Zhang

Liu

et al. 2019

ChemistrySelect

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This research investigates the extraction of fulvic acid (FA) from Hami lignite from Xinjiang, China, by microwave-hydrogen peroxide and microwave-hydrogen peroxide-formic acid methods. The effects of reaction time, oxidant concentration, oxygen-coal ratio and microwave power on FA yield are determined. Sulfuric acid-acetone was applied to purify the primary FA products. The results indicate that the oxidant concentration is a sensitive factor affecting the yield of FA, while reaction time has little effect. Formic acid can promote the extraction of FA from lignite by hydrogen peroxide, and under optimal experimental conditions, the yields of FA were 5.34% and 9.13%, respectively. UV-Vis analysis shows that FA has a lower aromaticity and degree of carbonyl conjugation in the presence of formic acid. Additionally, Fourier transform infrared spectroscopy was used to characterize the purified FA. The results show that the benzene rings of FA have one, two, three and four substitutions, oxygen-containing functional groups containing -COOH, C=O, -OCH 3 , CÀ OÀ C, Ar-OH, and R-OH and, furthermore, primary oxygen-containing functional groups consisting of C=O and C-OH. The aliphatic functional groups contain symmetrical and anti-symmetrical stretching vibrations of -CH 2 and -CH 3 . Finally, the hydrogen bonds are mainly composed of phenolic hydroxyls and alcoholic hydroxyls, while some hydrogen carboxylate is present.

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“…Kishor et al [8] suggested that humic substances, consisting of 60-80% fulvic acids, have anticarcinogenic properties. They may be beneficial in cancer therapy due to their heavy metal chelating properties, binding of proteins delivering anticancer drugs, and inhibition of cancer cell proliferation [9][10][11]. Fulvic acids are also good free radical scavengers.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Fulvic Acid Beverages by Mineral Profile and Antioxidant Capacity

Swat

Rybicka

Gliszczyńska‐Świgło

2019

Foods

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The main purpose of the study was to investigate the quality of fulvic acid-based food products. The concentrations of Ca, K, Mg, Na, Cu, Fe, Mn, and Zn, and antioxidant capacities of fulvic acid concentrates and ready-to drink beverages available on the global market were determined. The concentrations of minerals were determined using microwave plasma-atomic emission spectrometry. Antioxidant capacity was expressed as total polyphenol (TP) and flavonoid (TF) contents, the trolox equivalent antioxidant capacity (TEAC) and ferric reducing ability of plasma (FRAP) values. The daily portion of eight out of 14 products realized 45-135% of recommended daily allowance (RDA) for Fe. One of ready-to-drink beverages was also a good source of Mg (about 40% of RDA), and another one of Mn (about 70% of RDA). The concentrations of TP and TF in ready-to-drink beverages varied from 6.5 to 187 µg/mL, whereas in concentrates, from 5886 to 19,844 µg/mL. Dietary supplements or food products with fulvic acids may be a good source of antioxidant polyphenolic compounds and some minerals.

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Studies on the binding of fulvic acid with transferrin by spectroscopic analysis

Cited by 21 publications

References 32 publications

Delivery of fluorescent-labeled cyclodextrin by liposomes: role of transferrin modification and phosphatidylcholine composition

Delivery of fluorescent-labeled cyclodextrin by liposomes: role of transferrin modification and phosphatidylcholine composition

Extraction and Functional Group Characterization of Fulvic Acid from Hami Lignite

Characterization of Fulvic Acid Beverages by Mineral Profile and Antioxidant Capacity

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