Studies on the binding behavior of prodigiosin with bovine hemoglobin by multi-spectroscopic techniques

Tang, Jing; Yang, Chao; Zhou, Lin; Ma, Fei; Liu, Shuchao; Wei, Shaohua; Zhou, Jiahong; Zhou, Yecheng

doi:10.1016/j.saa.2012.05.059

Cited by 34 publications

(8 citation statements)

References 54 publications

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“…Figure (B) shows that β / α ratio is greater in the presence of TBO. The considerable changes in α ‐helix and β ‐sheet indicate that some α ‐helix structures have been converted into the β ‐sheet structures, while TBO is conjugated with lysozyme . The outcome from FT‐IR spectral analysis revealed that the binding of TBO to lysozyme protein has brought out considerable alteration in the structure of lysozyme.…”

Section: Resultsmentioning

confidence: 99%

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Elucidation of Binding Mechanism of Photodynamic Therapeutic Agent Toluidine Blue O with Chicken Egg White Lysozyme by Spectroscopic and Molecular Dynamics Studies

Shanmugaraj

Umadevi

Lakshmipathi

et al. 2017

Photochem & Photobiology

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The nature of binding mechanism of toluidine blue O (TBO) with chicken egg white lysozyme was studied comprehensively by various spectroscopic and computational methods. Both steady state and time-resolved fluorescence studies unambiguously point to the prevalence of static quenching mechanism in lysozyme-TBO system. Thermodynamic parameters revealed that the association of TBO with lysozyme was a spontaneous process in which hydrophobic and hydrogen bond interactions played a pivotal role in the binding process. The secondary and tertiary conformational changes of lysozyme in the presence of TBO were unraveled using absorption, Fourier transform infrared spectroscopy (FT-IR) and circular dichroism (CD) techniques. Molecular docking studies of lysozyme-TBO system substantiated the findings of site marker experiment and revealed TBO adjacent to Trp-63 and Trp-108 residues of lysozyme. Molecular dynamics (MD) simulation studies of lysozyme-TBO system indicate a stable and effective complexation of TBO with lysozyme. It is hoped that the results presented here will enable further understanding of TBO toxicity.

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Section: Resultsmentioning

confidence: 99%

“…structural changes in lysozyme (94). Gaussian multipeak fitting techniques are utilized to deconvoluting amide-I band (95). The curve fitting spectra of the normalized amide-I peak of lysozyme and lysozyme-TBO system are displayed in Fig.…”

Section: Conformational Studiesmentioning

confidence: 99%

Elucidation of Binding Mechanism of Photodynamic Therapeutic Agent Toluidine Blue O with Chicken Egg White Lysozyme by Spectroscopic and Molecular Dynamics Studies

Shanmugaraj

Umadevi

Lakshmipathi

et al. 2017

Photochem & Photobiology

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“…With the introduction of the ligand Yoh, there was considerable increase in the absorbance spectrum at 275 nm, this region depicts the phenyl groups of tryptophan and tyrosine residues in haemoglobin and the subtle decrease in absorbance at the 405 nm region is due to the absorption of hemachrome. [ 28,29 ] The presence of two isosbestic points at 315 and 354 nm was also recorded, indicating an equilibrium between the free drug and the bound drug with protein. [ 30 ] In the inset in Figure 2, the changes in the 405 nm peaks for Hb were amplified and presented.…”

Section: Resultsmentioning

confidence: 99%

“…For dynamic quenching, there is an increase in the effective collision numbers, enhancing the transfer of energy, and making the quenching constant of the fluorescence substance augment with the rise in temperature. [ 29 ] The experiment was performed by exciting the drug Yoh sample at 250 nm. The emission spectrum showed the λ max at 357 nm (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Protein‐binding characteristics of yohimbine, a natural indole alkaloid‐based drug for erectile dysfunction

2022

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Even today, talking about sexual dysfunction largely remains a taboo. Therefore less studies have been recorded and fewer remedies given. Erectile dysfunction (ED) is one of the most commonly treated psychological disorders that leads to major distress, interpersonal limitation and reduces the quality of life and marriage. This study aimed to assess a plant‐derived molecule, yohimbine (Yoh; a β‐carboline indole alkaloid often used for ED treatment) and its potential binding phenomenon with haemoglobin (Hb). Successful binding of Yoh with Hb is evident from spectroscopic and molecular‐docking results. Yoh quenched the fluorescence of Hb efficiently through a static mode. The binding affinity was in the order of 105 M−1 with 1:1 stoichiometry. Thermodynamic analyses concluded that the protein–ligand association was spontaneous and was attributed to entropy‐driven exothermic binding. Nonpolyelectrolytic factor was the core, dominating factor. Structural aspects were deciphered through infrared spectroscopy and computational methods. The giant 3D‐protein moiety was significantly perturbed through drug binding. Hydrophobic forces and hydrogen bonding participation were stipulated by molecular modelling data. This study reveals the detailed interaction pattern and molecular mechanism of Hb–Yoh binding, correlating the structure–function relationship for the first time, and therefore holds enormous importance from the standpoint of rational and efficient drug design and development.

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“…The spectral properties of prodigiosin ( 2 ) have a fascinating history as biological markers in the development of germ warfare and play a potential role in religious observations (bleeding statues and bread). 16 Recent studies have shown that prodigiosin's fluorescence is environmentally sensitive, responding to DNA 17 and protein 18 binding. An elegant application of this method was recently demonstrated for the in situ quantification of prodigiosin biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

A two-step resin based approach to reveal survivin-selective fluorescent probes

et al. 2021

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Studies on the binding behavior of prodigiosin with bovine hemoglobin by multi-spectroscopic techniques

Cited by 34 publications

References 54 publications

Elucidation of Binding Mechanism of Photodynamic Therapeutic Agent Toluidine Blue O with Chicken Egg White Lysozyme by Spectroscopic and Molecular Dynamics Studies

Elucidation of Binding Mechanism of Photodynamic Therapeutic Agent Toluidine Blue O with Chicken Egg White Lysozyme by Spectroscopic and Molecular Dynamics Studies

Protein‐binding characteristics of yohimbine, a natural indole alkaloid‐based drug for erectile dysfunction

A two-step resin based approach to reveal survivin-selective fluorescent probes

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