Studies on the Bacteriophage 2 Receptors of
            <i>Pseudomonas aeruginosa</i>

Castillo, Francisco; Bartell, Pasquale F.

doi:10.1128/jvi.14.4.904-909.1974

Cited by 24 publications

(15 citation statements)

References 26 publications

(21 reference statements)

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“…A chemical analysis revealed several differences among the GLPs of the three strains. With respect to amino sugar content, the present results differed markedly from the comparable results of Castillo and Bartell (11). Whereas we found no significant reduction of the total GLP amino sugar from the wild-type strain EI to the mutant EI/8s17, amino sugars are entirely absent from the analogous mutant of Castillo and Bartell.…”

Section: Resultscontrasting

confidence: 99%

“…1). An increase in phage was first measured at 60 min; depolymerase was first de- Previous studies of phage-related modifications of P. aeruginosa have shown significant changes in the amino sugar content of the GLP of the modified cell (11). Therefore, we investigated this component further.…”

Section: Resultsmentioning

confidence: 99%

“…3). The mean LD50s for the GLP layers from strains El and EI(8)3, injected intraperitoneally in mice, were both 23 ug per g of mouse, i.e., nearly three times greater than the LD5os for either the GLP from the wild-type strain EI or strain EI(8)3. though the purified slime GLP from the wildtype strain BI inactivates phage 2, the GLP from the lysogenized strain has lost this capacity (11). Relative to the wild-type strain BI, the slime GLP of the lysogenized strain contains less amino sugar.…”

Section: Resultsmentioning

confidence: 99%

“…Previous work from various laboratories has demonstrated that changes in phage sensitivity and serotype can occur following lysogenization of P. aeruginosa in vivo and in vitro (9,11,20,22). Our results extend these findings by demonstrating that lysogenization can effect considerable changes in the chemical composition and the antigenicity of the slime GLP and, perhaps more significantly, in the pathogenicity of the viable cell.…”

Section: Resultsmentioning

confidence: 99%

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Phage-related surface modifications of Pseudomonas aeruginosa: effects on the biological activity of viable cells

Dimitracopoulos¹,

Bartell²

1979

Infect Immun

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Lysogenic [EI(8)3] and phage 8-resistant mutant (EI/8s17) strains of Pseudomonas aeruginosa EI were isolated. Besides lacking the capacity to adsorb phage 8, strains EI(8)3 and EI/8s17 did not contain surface substrate for the depolymerase that is produced de novo when phage 8 infects wild-type strain EI. The glycolipoprotein (GLP) in the wild type contains phage 8 receptors and surface substrate for the depolymerase, as well as possesses characteristics of a virulence factor; therefore, the chemical and biological characteristics of the derived strains were investigated. The neutral-sugar, amino sugar, and protein content of the GLPs from the derived strains differed quantitatively from that of the wild type. In spite of some cross-reactivity, the GLPs from all strains were antigenically distinct in the indirect hemagglutination inhibition test. In mice, the toxicity of the GLP from strain EI(8)3 equaled that of the wild type, but the GLP of strain EI/8s17 was threefold less toxic. Significantly fewer viable EI(8)3 cells were required for the mouse 50% lethal dose than for the cells of either the wild type or the phage-resistant mutant.

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Section: Resultscontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Phage-related surface modifications of Pseudomonas aeruginosa: effects on the biological activity of viable cells

Dimitracopoulos¹,

Bartell²

1979

Infect Immun

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“…Several studies have shown that Pseudomonas aeruginosa undergoes 0-antigen conversion after infection by a variety of phages (7,13,14,17,37,43,44). Castillo and Bartell, using phage 2, have shown that lipopolysaccharide (LPS) is utilized as the phage receptor and that LPS from a phage 2-lysogenized strain shows a reduction in phage-inactivating capacity (12,13). This study was interesting in that the phage also appeared to be inactivated by slime, as well as by LPS, thereby suggesting that it may use both for adsorption onto the host (6, 13).…”

mentioning

confidence: 99%

O-antigen conversion in Pseudomonas aeruginosa PAO1 by bacteriophage D3

Kuzio¹,

Kropinski²

1983

J Bacteriol

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The lysogenization of Pseudomonas aeruginosa PAO by phage D3 results in derivatives which are resistant to superinfection by phage D3c by virtue of the fact that homologous phage cannot adsorb to these cells. The serologically and morphologically unrelated phage E79 showed a markedly decreased adsorption rate to the lysogen PAO(D3). Since both of these phages are lipopolysaccharide specific, these results suggested lysogenic conversion of the phage receptor. The lipopolysaccharide was extracted from strain PAO by the hot phenol-water technique, but this procedure was ineffective with PAO(D3). We developed a technique involving cold trichloroacetic acid extraction, followed by ultracentrifugation, digestion of the high-speed pellet with proteinase K, and ultimate purification on CsCl step gradients. The lipopolysaccharide from the wild type had inactivating activity against D3 and E79, whereas that from PAO(D3) inactivated neither. Chromatographic analysis indicated that the convertant lipopolysaccharide was smooth, and quantitative chemical analyses of the two preparations showed no differences in the level of the major fatty acids, amino compounds, or neutral sugars. On the other hand, sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the side chains had a decreased migration rate through the gel matrix. The application of 1H and 13C nuclear magnetic resonance spectroscopic analysis revealed that the PAO side chain is chemically identical to that of serotype O:2a,d, containing 2,3-(1-acetyl-2-methyl-2-imidazolino-5,4)-2,3-dideoxy-D-mannuronic acid, 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid, and 2-acetamido-2,6-dideoxy-D-galactose (D-fucosamine). The molecular basis of the conversion event was (i) the introduction of an acetyl group into position 4 of the fucosamine residue and a change in the bonding between trisaccharide repeating units from alpha 1 leads to 4 to beta 1 leads to 4.

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Isolation and partial characterization of ΦSP‐1, a Salmonella specific lytic phage from intestinal content of broiler chicken

Augustine

Louis²,

Varghese³

et al. 2012

J. Basic Microbiol.

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Salmonella enterica subsp. enterica serovar Enteritidis is a major causative agent of gastroenteritis with contaminated eggs and chicken meat being the major source of infection. Phages are seriously being considered as a safe and cheaper alternative to antibiotics. The intestinal content of chicken was used as source for isolating phages. Phage designated as ΦSP‐1 was selected for the study. Transmission electron microscopy (TEM) of phage ΦSP‐1 revealed that it belonged to family Podoviridae. The optimal multiplicity of infection (MOI) was 5 phages/cell. Latent and rise period were calculated to be 30 and 55 minutes respectively, while burst size was 44 phages/bacterial cell. The genome size of ΦSP‐1 was estimated to be 86 kb from pulsed‐field gel electrophoresis analysis (PFGE). The effect of different physical and chemical parameters like temperature, pH, salinity and CaCl2 were analyzed to optimize the conditions for large scale production of phages and to check the viability of ΦSP‐1 under different physiochemical conditions. A temperature of 40 °C, pH 8 and 0.25 M NaCl were found to be optimum for phage adsorption and it was able to survive up to a temperature of 50 °C for 3 min. Capability to survive under hostile environmental conditions, absence of virulence genes in genome and genus specificity suggest suitability of ΦSP‐1 to be used as a biocontrol agent.

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Studies on the Bacteriophage 2 Receptors of Pseudomonas aeruginosa

Cited by 24 publications

References 26 publications

Phage-related surface modifications of Pseudomonas aeruginosa: effects on the biological activity of viable cells

Phage-related surface modifications of Pseudomonas aeruginosa: effects on the biological activity of viable cells

O-antigen conversion in Pseudomonas aeruginosa PAO1 by bacteriophage D3

Isolation and partial characterization of ΦSP‐1, a Salmonella specific lytic phage from intestinal content of broiler chicken

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