Studies on the Bacterial Flora of the Alimentary Tract of Pigs. Ii. Streptococci: Selective Enumeration and Differentiation of the Dominant Group

Raibaud, P.; Caulet, Morgane; Galpin, J. V.; Mocquot, G.

doi:10.1111/j.1365-2672.1961.tb00262.x

Cited by 167 publications

(66 citation statements)

References 42 publications

(36 reference statements)

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“…The histamine producer Lb. buchneri B301 was grown in MRS (Oxoid), or LAPTg agar or broth (Raibaud et al, 1961), without aeration, at 37 uC. When indicated, LAPTg was supplemented with 2 g histidine l 21 (LAPTgHis).…”

Section: Methodsmentioning

confidence: 99%

Sequencing, characterization and transcriptional analysis of the histidine decarboxylase operon of Lactobacillus buchneri

et al. 2005

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Section: Methodsmentioning

confidence: 99%

Sequencing, characterization and transcriptional analysis of the histidine decarboxylase operon of Lactobacillus buchneri

et al. 2005

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“…All bacteria were stored as previously described (22). Before experimental use, cultures were propagated twice in MRS (Britania, Buenos Aires, Argentina) for lactobacilli or in LAPTg (27) for streptococci and enterococci.…”

mentioning

confidence: 99%

Diversity of Heteropolysaccharide-Producing Lactic Acid Bacterium Strains and Their Biopolymers

Mozzi

Vaningelgem

Hébert

et al. 2006

Appl Environ Microbiol

124

102

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Thirty-one lactic acid bacterial strains from different species were evaluated for exopolysaccharide (EPS) production in milk. Thermophilic strains produced more EPS than mesophilic ones, but EPS yields were generally low. Ropiness or capsular polysaccharide formation was strain dependent. Six strains produced high-molecular-mass EPS. Polymers were classified into nine groups on the basis of their monomer composition. EPS from Enterococcus strains were isolated and characterized.Certain lactic acid bacteria (LAB) produce exopolysaccharides (EPS), either capsular polysaccharides (CPS) that are tightly associated with the cell surface or slime EPS that are secreted into the extracellular environment. EPS from LAB can be divided into homopolysaccharides, which are polymers composed of one type of monosaccharide, and heteropolysaccharides (HePS), which are polymers of repeating units that are composed of two or more types of monosaccharides (5,6,7,11). A large biodiversity of HePS from LAB exists regarding their composition and structure, molecular mass (MM), yield, and functionalities (5,7,35). Further, polymer formation is strongly influenced by culture conditions (1,7,22,32). Recently, the molecular genetics of HePS biosynthesis have been studied for different LAB species (6, 25). Several glycosyltransferases involved in the assemblage of the HePS repeating units have been discovered (17,18,35). EPS can act as viscosifying, stabilizing, gel-forming, and/or water-binding agents in various foods (6, 10). Additionally, they have been claimed to display properties beneficial to health (6, 29). Little attention has been paid to CPS formation by food grade LAB. Exploration of the biodiversity of wild LAB strains is the most suitable approach to search for a desired EPS phenotype (28,33,34). The aims of this study were to seek new EPS-producing LAB strains, to characterize their biopolymers, and to explore their diversity to find novel or interesting HePS or HePS-producing strains.EPS screening, isolation, and characterization. Two hundred one thermophilic (11 strains of Lactobacillus acidophilus, 79 of Lactobacillus delbrueckii subsp. bulgaricus, 1 of L. delbrueckii subsp. lactis, 1 of Streptococcus macedonicus, and 42 of Streptococcus thermophilus) and mesophilic (23 strains of Enterococcus faecalis, 1 of Enterococcus faecium, 5 of Lactobacillus casei, 29 of Lactobacillus paracasei, and 9 of Lactobacillus rhamnosus) LAB strains (CERELA Culture Collection, Tucumán, Argentina) were used throughout this study. All bacteria were stored as previously described (22). Before experimental use, cultures were propagated twice in MRS (Britania, Buenos Aires, Argentina) for lactobacilli or in LAPTg (27) for streptococci and enterococci.Screening for EPS-producing LAB was performed with reconstituted skim milk (10%, wt/vol) at 37°C for 16 h using cultures propagated in milk as the inoculum (1%, vol/vol). Noninoculated medium was used as a control. Cell counts, expressed as numbers of CFU per milliliter, were determined by pour pla...

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“…The base medium for the growth of this microorganism was the one described by RAIBAUD et al (10). It is composed of yeast extract, 1.0 % ; peptone, 1.5 % ; tryptone, 1.0 %; glucose, 1.0 % and Tween 80, 0.1 % (LAPTg).…”

Section: Methodsmentioning

confidence: 99%

Aspartate metabolism in Lactobacillus murinus CNRS 313. I. Aspartase.

Rollán¹,

Nadra²,

Holgado³

et al. 1985

J. Gen. Appl. Microbiol.

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Aspartase from Lactobacillus murinus CNRZ 313 (L-aspartate ammonia lyase, E.C. 4.3.1.1.), is one of the enzymes of aspartate metabolism in lactic acid bacteria. The growth of this microorganism in LAPTg medium is not altered by the addition of L-aspartate. Aspartase is a thermolabile enzyme. Its activity was not changed in one month at temperatures lower than 0°C, independently of the presence of 10 glycerol or 0.05 M MgCl2. Maximum activity occurred at 32°C and pH 7.5 in 0.15 M Tris-HC1 pH 7.5. At pH values different from the optimum, positive cooperation between substrate molecules was observed.The Km values for L-aspartate at pH 7.5 and 7.0 were: 3.7x 10-2 M and 9.0 x 10-2 M respectively. The pK values of pKa 6.75 and pKb 7.35 were calculated from Dixon plots. The 4G* of the reaction was calculated by Arrhenius plot. The values were 4,807 cal moll below 25°C and 2,665 cal mold above 25°C. Both 10-3 M HgC12 and NaCN had inhibitory effects.

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Studies on the Bacterial Flora of the Alimentary Tract of Pigs. Ii. Streptococci: Selective Enumeration and Differentiation of the Dominant Group

Cited by 167 publications

References 42 publications

Sequencing, characterization and transcriptional analysis of the histidine decarboxylase operon of Lactobacillus buchneri

Sequencing, characterization and transcriptional analysis of the histidine decarboxylase operon of Lactobacillus buchneri

Diversity of Heteropolysaccharide-Producing Lactic Acid Bacterium Strains and Their Biopolymers

Aspartate metabolism in Lactobacillus murinus CNRS 313. I. Aspartase.

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