Studies on the antimicrobial activity of Nigella sativa seed (black cumin)

Hanafy, M.; Hatem, M.E.

doi:10.1016/0378-8741(91)90047-h

Cited by 306 publications

(191 citation statements)

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“…The anti-microbial effects of N. sativa seeds against different pathogenic microbes were investigated. The diethyl ether extract was found to cause concentrationdependent inhibition of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and a pathogenic yeast Candida albicans [14] . The methanol and chloroform extracts have high inhibitory effects against S. aureus, P. aeruginosa and C. albicans [15] .…”

Section: Introductionmentioning

confidence: 99%

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Abu-Al-Basal¹

2009

American J. of Applied Sciences

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Problem statement:The developing microbial resistance to the existing anti-microbial agents has become a real challenge and a serious problem facing patients suffering from skin infections. Seeds of Nigella sativa have been used for a long time in folk medicine for the treatment of such infections. Production of new potent agents is urgently needed, especially for hospitals and health centers. Therefore, the anti-microbial effect of aqueous, diethyl ether, chloroform and petroleum ether extracts of the seeds against four standard microbial strains and seven clinical isolates from patients with skin wound infections were investigated. Approach: The in vitro anti-microbial effect of the extracts at a concentration of 20% on standard strains and clinical isolates was assessed and compared with standard drugs, chloramphenicol and amphotericin B using agar well diffusion assay. The in vivo anti-bacterial effect of petroleum ether extract was studied in male BALB/c mice infected subcutaneously with S. aureus (ATCC 25923) or a clinical isolate (0.1 mL from 10 9 colony forming units mL −1 suspension) and immediately treated at the infected site by subcutaneous injection of 0.1 mL of pure extract (fixed oil) or chloramphenicol or normal saline. Counts of viable bacteria present in the skin area corresponding to the infected site were determined, after 24 and 48 h of infection and treatment. Results: The aqueous extract did not show any inhibitory effect against all the tested microorganisms. The diethyl ether and chloroform extracts indicated significant inhibitory effect only against Gram-positive bacteria. However, petroleum ether extract was proved to be the most powerful one against these bacteria and also against other clinical isolates like one Gram-negative bacterium (Klebsiella pneumonia) and the yeast (Candida albicans). Moreover, the extract revealed a superior effect over the standard drug, chloramphenicol, on the clearance of subcutaneous staphylococcal infection in mice when injected at the site of infection. Counts of viable bacteria were decreased at highly significant level in mice infected with S. aureus (ATCC 25923) or a clinical isolate. Conclusion/Recommendations:The results of this study revealed clear potentiality of N. sativa fixed oil as a source for anti-microbial drugs and support its use in folk medicine for the treatment of microbial skin infections.

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Section: Introductionmentioning

confidence: 99%

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Abu-Al-Basal¹

2009

American J. of Applied Sciences

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“…These studies have showed a wide spectrum of activities such as antibacterial (Ferdous et al, 1992;Hanafy and Hatem, 1991;Rathee et al, 1982), antitumor (David et al, 1998), anti-inflammatory (Houghton et al, 1995Mutabagani and El-Mahdy, 1997), CNS depressant and analgesic (Khanna et al, 1993), hypoglycemic (Al-Hader et al, 1993), smooth muscles relaxant (Aqel, 1993;Aqel, 1995;Aqel and Shaheen, 1996) cytotoxic and immunostimulant (Swamy and Tan, 2000). Some of these activities have been predominantly attributed to the volatile and fixed oils.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition of the Fixed and Volatile Oils of Nigella sativa L. from Iran

Nickavar

Mojab

Javidnia

et al. 2003

Zeitschrift Für Naturforschung C

260

135

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The chemical composition of the extracted fixed oil (total fatty acid composition) and volatile oil of Nigella sativa L. seeds grown in Iran were determined by GC and GC/MS. Eight fatty acids (99.5 %) and thirty-two compounds (86.7 %) have been identified in the fixed and volatile oils, respectively. The main fatty acids of the fixed oil were linoleic acid (55.6 %), oleic acid (23.4 %), and palmitic acid (12.5 %). The major compounds of the volatile oil were trans-anethole (38.3 %), p-cymene (14.8 %), limonene (4.3 %), and carvone (4.0 %).

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“…While black cumin has antibacterial, antioxidant and anti-inflammatory effects [158-395 160], stinging nettle possess antimicrobial activity with effectiveness against a wide range of 396 microorganisms [104]. These supplements induced an increase in lysozyme, myeloperoxidase and 397 antiprotease activities, and total serum protein and IgM levels [161].…”

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confidence: 99%

The response of fish to immunostimulant diets

Vallejos‐Vidal

Reyes-López

Teles

et al. 2016

Fish & Shellfish Immunology

264

110

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8 Fish Immunostimulation through dietary manipulation 9The aquaculture sector has shown a rapid growth over the last 30 years with an associated increase in 10 disease problems as result of rapid expansion and amongst other factors high stocking densities. In order 11to maintain fish health and to improve performance immunostimulants have been used as dietary 12 additives to improve weight gain, feed efficiency, and/or disease resistance in cultured fish. 13An immunostimulant is a natural or chemical substance that stimulates the immune system by specific 14(vaccines or antigens) or non-specific (irrespective of antigenic specificity) routes. In Aquaculture, non- 21The immunostimulant effect of dietary supplements in fish has been focused mainly on the evaluation of 22 non-specific immune parameters and, therefore, on the consequences of these treatments on the innate 23 immune system. The innate immune system has both cellular and humoral components by which it carries 24 out its protective function. The major components of the innate immune system at cellular level are 25 leukocytes, mainly monocytes, macrophages and granulocytes [1,2]. Among the granulocytes, neutrophils 26are the most abundant cell-type and their presence has been described in Salmoniformes, Cypriniformes 27 and Perciformes [3]. Neutrophils and macrophages are responsible for the production of bioactive 28 molecules for pathogen recognition and destruction, cellular communication and activation, initiation of 29 an adaptive immune response and later, resolution of an inflammatory response and tissue repair. 30Furthermore, these cell types are responsible in the majority for phagocytosis [4], one of the main 31 mediators of innate immunity to remove pathogens such as bacteria, viruses, and parasites. For this 32 reason, these immune cell types are also called phagocytes. This microbe/killing mechanism triggers 33 diverse antimicrobial processes that use a wide variety of mechanisms including cellular activation, 34 production of oxidative radicals, and the production of cytokines driving the inflammatory response 35 amongst others. 36Two of the most important antimicrobial systems of phagocytic cells are the NADPH phagocyte oxidase 37 and inducible nitric oxide synthase (iNOS) pathways, which are responsible for the generation of 38 superoxide (O2 − ) and nitric oxide (NO) radicals, respectively. NADPH oxidase, a multi-subunit complex, 39 catalyses a one-electron reduction of molecular oxygen into superoxide anion (O2 − ), also referred to as 40 reactive oxygen species (ROS), which is either spontaneously converted to H2O2 or enzymatically by 41 superoxide dismutase (SOD). In comparison to neutrophils, the size of the respiratory burst is much 42 reduced in macrophages [5]. Since O2 − is the first product to be released from the respiratory burst, the 43 measurement of O2 − has been accepted as a direct and accurate way of measuring respiratory burst 44 activity [6]: the reduction of ferricytochrome c to determine extracellular O2 − , and th...

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Studies on the antimicrobial activity of Nigella sativa seed (black cumin)

Cited by 306 publications

References 0 publications

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Chemical Composition of the Fixed and Volatile Oils of Nigella sativa L. from Iran

The response of fish to immunostimulant diets

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