Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSn

Hao, Yue; Pi, Zifeng; Song, Fengrui; Liu, Zhiqiang; Cai, Zongwei; Liu, Shuying

doi:10.1016/j.talanta.2008.10.022

Cited by 96 publications

(101 citation statements)

References 15 publications

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“…In Table 1, HPLC-ESI-MS analysis shows the identified alkaloids in Fuzi total alkaloid (see ion chromatograms in Supplementary Figures, available in the online version only), and these data are consistent with those in the previous report (24). Figure 1, A and B shows that 7-day repeated administration of Fuzi total alkaloid has no effect on body weight in the normal and ovariectomized mice.…”

Section: Hplc-esi-ms Analysissupporting

confidence: 88%

“…Fuzi was purchased from a traditional Chinese medicinal store and was identified as reported (22). Fuzi total alkaloid was extracted according to a previous report (23,24) and ingredient analysis was performed by Dr. Yue's group who reported the ingredient analysis of Fuzi alkaloids before (24).…”

Section: Drugsmentioning

confidence: 99%

“…The flow rate was set at 0.3 ml/min and the column temperature was maintained at 23°C. Total alkaloid was dissolved in chloroform plus methanol (1:1) and loaded to the column as previously reported (24).…”

Section: Hplc-esi-ms Analysismentioning

confidence: 99%

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Antidepressant-like Effect of Fuzi Total Alkaloid on Ovariectomized Mice

Liu

Zhou

et al. 2012

J Pharmacol Sci

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Abstract. Recent studies in vivo and vitro have shown that Fuzi polysaccharide has an antidepressant-like effect. Polysaccharide and total alkaloid are the two most important components of Fuzi. However, little is known about the antidepressant-like effect of Fuzi total alkaloid. To investigate the antidepressant-like effect of Fuzi total alkaloid, behavioral studies were performed in the open field test and forced swimming test. Repeated intragastric administration of Fuzi total alkaloid for 7 days (10 mg/kg) to normal mice decreased immobility time compared to the vehicle group. Furthermore, repeated administration of Fuzi total alkaloid (10 or 30 mg/kg) to ovariectomized mice also decreased immobility time in a dose-dependent manner. However, these antidepressant-like behavioral effects were not simply due to locomotor hyperactivity. Further experiments showed that Fuzi total alkaloid enhanced the ratio of phospho-CREB/CREB (cAMP response element-binding) and BDNF (brain-derived neurotrophic factor) protein level in the frontal cortex and hippocampus in ovariectomized mice but not in normal mice. These results indicate that the CREB-BDNF pathway may be involved in the antidepressant-like effect of Fuzi total alkaloid in ovariectomized mice.

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Section: Hplc-esi-ms Analysissupporting

confidence: 88%

Section: Drugsmentioning

confidence: 99%

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Antidepressant-like Effect of Fuzi Total Alkaloid on Ovariectomized Mice

Liu

Zhou

et al. 2012

J Pharmacol Sci

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“…Typical examples of dereplication strategies for LC-MS analysis of alkaloids are colchicinoids [102], pyrrolizidine alkaloids and their corresponding N-oxides [103], aconitum alkaloids [104] and alkaloids in Catharanthus roseus roots [105]. Based on the combination of retention time, UV and mass spectra, known compounds were rapidly dereplicated and new structures were identified.…”

Section: Alkaloidsmentioning

confidence: 99%

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

161

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“…So, the determination of alkaloids in P. amurense and P. chinense is highly desirable to ensure authenticity, stability, and safety of the medicinal plants. Up to now, several assays have been described for the determination of alkaloids based on GC-MS [7], TLC [8], HPLC-UV [9], HPLC-MS/MS [10][11][12], and CE [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of six alkaloids inPhellodendron amurenseby high-performance liquid chromatography

Li¹,

Liu²,

Shi

2013

Acta Chromatographica

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Summary.A high-performance liquid chromatography (HPLC) method has been developed for simultaneous determination of six alkaloids, i.e., (−)-(R)-platydesmin, noroxyhydrastinine, berberine, skimmianine, canthin-6-one, and pteleine in the herbal medicine of Phellodendron amurense Rupr. The optimal condition for extraction and separation was achieved with a linear mobile phase gradient prepared from 0.1% phosphoric acid and acetonitrile. The LODs and LOQs for the analytes ranged from 0.06 to 0.22 μg mL −1 and from 0.25 to 0.80 μg mL −1 , respectively. The optimized method was applied to the determination of alkaloids in P. amurense Rupr. and was found to be efficient. This method can provide a scientific and technical platform to the manufacturers for setting up a quality control standard as well as to the public for quality and safety assurance of the proprietary traditional Chinese medicines.

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Studies on the aconitine-type alkaloids in the roots of Aconitum Carmichaeli Debx. by HPLC/ESIMS/MSn

Cited by 96 publications

References 15 publications

Antidepressant-like Effect of Fuzi Total Alkaloid on Ovariectomized Mice

Antidepressant-like Effect of Fuzi Total Alkaloid on Ovariectomized Mice

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Simultaneous determination of six alkaloids inPhellodendron amurenseby high-performance liquid chromatography

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