Studies on Streptomyces Phage

Gilmour, C. M.; Noller, Eric C.; Watkins, Bruce E.

doi:10.1128/jb.78.2.186-192.1959

Cited by 11 publications

(3 citation statements)

References 7 publications

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“…For example, the mycelial nature of streptomycetes complicates quantitative studies. The notion of MOI loses a lot of its significance once mycelium has formed, as the network structure originating from one spore has greatly increased phage adsorption but would still be counted as one CFU [14,50]. Furthermore, the formation of clumps, although mitigated by the addition of glass beads or increase of osmotic pressure [51], makes accurate monitoring of cell growth (based on optical density or backscatter) difficult.…”

Section: Discussionmentioning

confidence: 99%

Genome Sequence and Characterization of Five Bacteriophages Infecting Streptomyces coelicolor and Streptomyces venezuelae: Alderaan, Coruscant, Dagobah, Endor1 and Endor2

Hardy

Sharma

Kever

et al. 2020

Viruses

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Streptomyces are well-known antibiotic producers, also characterized by a complex morphological differentiation. Streptomyces, like all bacteria, are confronted with the constant threat of phage predation, which in turn shapes bacterial evolution. However, despite significant sequencing efforts recently, relatively few phages infecting Streptomyces have been characterized compared to other genera. Here, we present the isolation and characterization of five novel Streptomyces phages. All five phages belong to the Siphoviridae family, based on their morphology as determined by transmission electron microscopy. Genome sequencing and life style predictions suggested that four of them were temperate phages, while one had a lytic lifestyle. Moreover, one of the newly sequenced phages shows very little homology to already described phages, highlighting the still largely untapped viral diversity. Altogether, this study expands the number of characterized phages of Streptomyces and sheds light on phage evolution and phage-host dynamics in Streptomyces.

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Section: Discussionmentioning

confidence: 99%

Genome Sequence and Characterization of Five Bacteriophages Infecting Streptomyces coelicolor and Streptomyces venezuelae: Alderaan, Coruscant, Dagobah, Endor1 and Endor2

Hardy

Sharma

Kever

et al. 2020

Viruses

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“…5,21 The low burst size could be the result of the morphological complexity of Streptomyces 21 . The meaning of MOI loses its signi cance once spores have germinated and a mycelium is formed as multiple phages are able to attach, but the mycelium would still be counted as one colony forming unit 13,22 .…”

Section: Discussionmentioning

confidence: 99%

Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus

Ongenae,

Kempff,

Neer

et al. 2023

Preprint

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Despite the rising interest in bacteriophages, little is known about their infection cycle and lifestyle in a multicellular host. Even in the model system Streptomyces, only a small number of phages have been sequenced and well characterized so far. Here, we report the complete characterization and genome sequences of Streptomyces phages Vanseggelen and Verabelle isolated fromStreptomyces coelicolor. A wide range of Streptomyces strains could be infected by both phages, but neither of the two phages was able to infect members of the closely related sister genus Kitasatospora. The phages Vanseggelen and Verabelle have a double-stranded DNA genome with lengths of 48,720 and 48,126 bp, respectively. Both phage genomes contain 72 putative genes, and the presence of an integrase encoding protein indicates a lysogenic lifestyle. Characterization of the phages revealed their stability over a wide range of temperatures (25–45°C) and pH values (4–10). In conclusion, Streptomyces phage Vanseggelen and Streptomyces phage Verabelle are newly isolated phages that can be classified as new species in the genus Camvirus, within the subfamily Arquattrovirinae.

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“…The phage 514-3 of Streptomyces griseus described in the preceding paper (Gilmour et al, 1959) has from the time of its isolation from soil, showed a high infectivity for a large number of diverse Streptomyces species. The application of pure-lining procedures involving transfers from plaques and continued propagation on the homologous host S. griseus strain 3475 has not altered the observed broad host range of the phage.…”

mentioning

confidence: 88%