Studies on Screening, Isolation and Purification of a Fibrinolytic Protease from an Isolate (VK12) of Ganoderma Lucidum and Evaluation of its Antithrombotic Activity

Kumaran, Sekar; Palani, P.; Nishanthi, Ramasami; Kaviyarasan, V.

doi:10.3314/jjmm.52.153

Cited by 15 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These inhibitory effects may involve the release of intrinsic ADP from platelets or the metabolites of AA and PAF. Other research showed that purified ganoderma fibrinolytic protease protected mice against thrombotic death or paralysis induced by collagen and epinephrine and also activated the partial thromboplastin time (APTT) and thrombin time (TT) in rat platelets (Kumaran et al, 2011). The polysaccharide of ganoderma was also found to prolong the blood clotting and bleeding time in rats and decrease the thrombosis and fibrinogen level in blood plasma (Klupp et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

In silico Approach for Anti-Thrombosis Drug Discovery: P2Y1R Structure-Based TCMs Screening

Sun

et al. 2017

Front. Pharmacol.

View full text Add to dashboard Cite

Cardiovascular diseases (CVDs), including thrombosis, which is induced by platelet aggregation, are the leading cause of mortality worldwide. The P2Y1 receptor (P2Y1R) facilitates platelet aggregation and is thus an important potential anti-thrombotic drug target. The P2Y1R protein structure contains a binding site for receptor antagonist MRS2500 within its seven-transmembrane bundle, which also provides suitable pockets for numerous other ligands to act as nucleotide antagonists of P2Y1R. The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) comprises 499 Chinese Pharmacopoeia-registered herbs and the structure information for 29,384 ingredients. In silico docking of these compounds into the P2Y1R protein structure within the MRS2500 pocket can identify potential antithrombotic drugs from natural medicinal plants. Docking studies were performed and scored to evaluate ligand-binding affinities. In this study, a total of 8987 compounds from Traditional Chinese Medicine (TCM) were filtered by Lipinski's rule of five, and their ideal oral-intake properties were evaluated. Of these, 1656 compounds distributed in 443 herbs docked into the P2Y1R-MRS2500 structure in 16,317 poses. A total of 38 compounds were ranked with a DockScore above 70, and these may have significant potential for development into anti-thrombosis drugs. These computational results suggested that licorice (Glycyrrhiza uralensis Fisch), cimicifugae (Cimicifuga foetida L.), and ganoderma (Ganoderma lucidum Karst) and their chemical constituents, which have not previously been widely used for anti-thrombosis, may have unexpected effects on platelet aggregation. Moreover, two types of triterpene scaffolds summarized from 10 compounds were distributed in these three herbs and also docked into P2Y1R. These scaffold structures may be utilized for the development of drugs to inhibit platelet aggregation.

show abstract

Section: Resultsmentioning

confidence: 99%

In silico Approach for Anti-Thrombosis Drug Discovery: P2Y1R Structure-Based TCMs Screening

Sun

et al. 2017

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…The Ganoderma species contain several important bio-active compounds, including polysaccharides, triterpenoids, immunomodulatory peptides, amino acids, alkaloids, and so on [ 17 , 18 , 19 ]. These compounds present antitumor, immunomodulatory, anti-thrombosis, cholesterol regulation, anti-hyperglycemic and antibacterial activities [ 20 , 21 , 22 , 23 ]. Most studies are related to the pharmacological effects of the triterpenoids and polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

Ganoderma tsugae Hepatoprotection against Exhaustive Exercise-Induced Liver Injury in Rats

Huang

Yang

et al. 2013

Molecules

View full text Add to dashboard Cite

Several studies have been shown that accelerated apoptosis is involved in post-exercise lymphocytopenia and tissue damage after high-intensity exercise. Ganoderma tsugae (GT) is one of the well-known medicinal mushrooms that possess various pharmacological functions. This mushroom has traditionally been used for health promotion purposes. This study investigates the hepatoprotective effects of GT on exhaustive exercise-induced liver damage. Twenty-four male Sprague-Dawley rats were randomly divided into four groups and designated as exhaustive exercise only (E), exhaustive exercise with low dosage (EL), medium dosage (EM) and high dosage (EH) GT at 0, 0.1875, 0.9375 and 1.875 g/kg/day, respectively. After 30 days all rats were euthanized immediately after an exhaustive running challenge on a motorized treadmill. The rat livers were immediately harvested. Evidence of apoptotic liver cell death was revealed using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and caspases mediated cascade events. DNA fragmentation, an apoptosis process, can be examined using TUNEL assay. A few TUNEL-positive hepatocytes, compared to the exercise only group, were observed in the livers from exhaustive animals supplemented with GT. Immunoblot analysis also showed that caspase-6-mediated specific cleavage of lamin A/C was increased significantly in the livers of group E, but was significantly decreased in the EM and EH groups. Our observations demonstrate that GT possesses anti-apoptotic and hepatoprotective potential after exhaustive exercise.

show abstract

“…G. lucidum may have a mild antithrombotic effect itself in high doses and this could increase the effect of other anticoagulant or antiplatelet medications, including aspirin (Kumaran et al. 2011 ), resulting in an increased risk of bruising or bleeding. In patients taking other prescription medications, it is generally better to separate the intake of those medications and G. lucidum products by at least two hours in case there is any interference with drug absorption.…”

Section: Adverse Effectsmentioning

confidence: 99%

The beneficial effects of Ganoderma lucidum on cardiovascular and metabolic disease risk

Chan

Tomlinson

Chan

2021

Pharmaceutical Biology

View full text Add to dashboard Cite

Context Various herbal medicines are thought to be useful in the management of cardiometabolic disease and its risk factors. Ganoderma lucidum (Curtis) P. Karst. (Ganodermataceae), also known as Lingzhi, has received considerable attention for various indications, including some related to the prevention and treatment of cardiovascular and metabolic disease by ameliorating major cardiovascular risk factors. Objective This review focuses on the major studies of the whole plant, plant extract, and specific active compounds isolated from G. lucidum in relation to the main risk factors for cardiometabolic disease. Methods References from major databases including PubMed, Web of Science, and Google Scholar were compiled. The search terms used were Ganoderma lucidum , Lingzhi, Reishi, cardiovascular, hypoglycaemic, diabetes, dyslipidaemia, antihypertensive, and anti-inflammatory. Results A number of in vitro studies and in vivo animal models have found that G. lucidum possesses antioxidative, antihypertensive, hypoglycaemic, lipid-lowering, and anti-inflammatory properties, but the health benefits in clinical trials are inconsistent. Among these potential health benefits, the most compelling evidence thus far is its hypoglycaemic effects in patients with type 2 diabetes or hyperglycaemia. Conclusions The inconsistent evidence about the potential health benefits of G. lucidum is possibly because of the use of different Ganoderma formulations and different study populations. Further large controlled clinical studies are therefore needed to clarify the potential benefits of G. lucidum preparations standardised by known active components in the prevention and treatment of cardiometabolic disease.

show abstract

Studies on Screening, Isolation and Purification of a Fibrinolytic Protease from an Isolate (VK12) of Ganoderma Lucidum and Evaluation of its Antithrombotic Activity

Cited by 15 publications

References 26 publications

In silico Approach for Anti-Thrombosis Drug Discovery: P2Y1R Structure-Based TCMs Screening

In silico Approach for Anti-Thrombosis Drug Discovery: P2Y1R Structure-Based TCMs Screening

Ganoderma tsugae Hepatoprotection against Exhaustive Exercise-Induced Liver Injury in Rats

The beneficial effects of Ganoderma lucidum on cardiovascular and metabolic disease risk

Contact Info

Product

Resources

About