Plant nutrition requires organic nitrogen to be mineralized before roots can absorb it. A 13-year field study was conducted on typical rain-fed Mediterranean Vertisol to determine the effects of tillage system, crop rotation and N fertilizer rate on the long-term NH 4 + -N content in the soil profile (0-90 cm). The experiment was designed as a randomized complete block with a split-split plot arrangement and three replications. The main plots tested the effects from the tillage system (no-tillage and conventional tillage); the subplots tested crop rotation with 2-year rotations (wheat-wheat, wheat-fallow, wheat-chickpea, wheat-faba bean and wheat-sunflower) and the subsubplots examined the N fertilizer rate (0, 50, 100 and 150 kg N/ha). Soil NH 4 + -N content was greatest in the rainiest years and greater under the no-tillage (NT) system than the conventional tillage (CT) system (57 and 48 kg/ha, respectively). The deepest soil (30-60 and 60-90 cm) contained a greater NH 4 + -N content (21.0 and 21.4 kg/ha, respectively) than the shallowest soil (19.5 kg/ha in 0-30 cm). This observation may be related to Vertisol characteristics, especially crack formation that allows greater mineralization in the deepest layers by displacing organic matter.