Studies on preparation of haddock calcium tablet and its biological utilization

Huo, Jiancong; Deng, Shanggui; Guo-zhong, Tong

doi:10.3724/sp.j.1231.2010.06663

Cited by 2 publications

(1 citation statement)

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“…The high cost of these procedures limited ALA microbial production and application (Chen et al, 2020 ). Large amounts of waste biomass, such as cassava residue (CR) (Wang et al, 2012 ) and fish waste (FW) (Huo et al, 2010 ), are produced annually in China, which could be cheap resources for microbial synthesis of value‐added products such as ALA (Yu et al, 2018 ), since the acid hydrolysates of CR and FW are rich in glucose (Cai et al, 2012 ), amino acids and growth factors (Samat et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Production of 5‐aminolevulinic acid from hydrolysates of cassava residue and fish waste by engineered Bacillus cereusPT1

Ying

Yang

et al. 2022

Microbial Biotechnology

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The economical production of 5‐aminolevulinic acid (ALA) has recently received increasing attention for its extensive use in agriculture. In this study, a strain of Bacillus cereus PT1 could initially produce ALA at a titre of 251.72 mg/L by using a hydrolysate mixture of low‐cost cassava residue and fish waste. The integration of endogenous hemA encoding glutamyl‐tRNA reductase led to a 39.30% increase in ALA production. Moreover, improving cell permeability by deletion of the LytR‐CpsA‐Psr (LCP) family gene tagU led to a further increase of 59.73% in ALA production. Finally, the engineered strain B. cereus PT1‐ hemA ‐Δ tagU produced 2.62 g/L of ALA from the previously mentioned hydrolysate mixture in a 7‐L bioreactor. In a pot experiment, foliar spray of the ALA produced by B. cereus PT1‐ hemA ‐Δ tagU from the hydrolysates increased salt tolerance of cucumber by improving chlorophyll content and catalase activity, while decreasing malondialdehyde content. Overall, this study demonstrated an economic way to produce ALA using a microbial platform and evidenced the potential of ALA in agricultural application.

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