Studies on polyamine biosynthesis in Euglena gracilis

Aleksijevic, A.; Grove, Jeffrey; Schuber, Francis

doi:10.1016/0005-2787(79)90096-0

Cited by 26 publications

(7 citation statements)

References 25 publications

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“…(2, 13, 16). Various polyamines were also found in plants (1,11,14).In animal cells, the polyamine putrescine is formed from Lornithine by ODC3 (2,12). In plants, however, the accepted notion is that putrescine is formed from arginine by ADC via agmatine (11,14,15).…”

mentioning

confidence: 99%

Ornithine Decarboxylase and Arginine Decarboxylase Activities in Meristematic Tissues of Tomato and Potato Plants

Cohen¹,

Heimer²,

Mizrahi³

1982

Plant Physiol.

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Ormithine decarboxylase and arginine decarboxylase activities were measured in roots and buds of tomato (Lycopersicon esculentum MiUl. cv. Pearson ms-35) and potato (Solanum tuberosum cv. Desire) plants. In both tomato and potato, the activity of ornithine decarboxylase was the highest at the root tip, decreasing proximally. The same was true for potato buds. In vegetative buds of tomato, the highest activity was found in the youngest leaves. The older the leaf, the lower was orithine decarboxylase activity. Arginine decarboxylase, on the other hand, did not display a similar gradient. These findings are in accordance with the suggestion that in tomato and potato elevated ornithine decarboxylase activity is associated with intense mitotic activity.Enhanced synthesis of polyamines in animal cells is associated with cell proliferation, cell differentiation, tissue regeneration, malignancy, etc. (2, 13, 16). Various polyamines were also found in plants (1,11,14).In animal cells, the polyamine putrescine is formed from Lornithine by ODC3 (2,12). In plants, however, the accepted notion is that putrescine is formed from arginine by ADC via agmatine (11,14,15). Recently, it was shown that elevated ODC activity is associated with rapid cell proliferation in two plant systems, tomato ovaries immediately after pollination and tobacco XD cells growing in suspension culture (8, 9). ADC was also present in tomato ovaries. However, its activity did not change during the logarithmic phase ofgrowth and was only 25% that ofthe maximal activity of ODC. It was, therefore, expected that elevated ODC activity would be associated with meristematic regions in these plants.This

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confidence: 99%

Ornithine Decarboxylase and Arginine Decarboxylase Activities in Meristematic Tissues of Tomato and Potato Plants

Cohen¹,

Heimer²,

Mizrahi³

1982

Plant Physiol.

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“…There have been relatively few studies, of their specificity with respect to the amine acceptors and some of the published literature is contradictory. Formation of sym-norspermine and sym-norspermidine by addition of aminopropyl groups to 1,3-diaminopropane occurs with enzymes isolated from thermophilic bacteria (De Rosa et al, 1978) and Euglena gracilis (Aleksijevic et al, 1979), but indirect evidence suggested that 1,3-diaminopropane was inactive with the enzyme from rat prostate (Hibasami & Pegg, 1978b). It has been claimed that 1,5-diaminopentane (cadaverine) is not a substrate for mammalian spermidine synthase (Kallio et al, 1977;Samejima & Nakazawa, 1980), whereas indirect evidence that, this diamine was a substrate was published by Hibasami & Pegg (1978a,b) and it is known to be converted into N-(3-aminopropyl)cadaverine by the enzyme from Escherichia coli (Dion & Cohen, 1972;Bowman et al, 1973).…”

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Specificity of mammalian spermidine synthase and spermine synthase

Pegg¹,

Shuttleworth²,

Hibasami³

1981

Biochemical Journal

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1. The specificity of rat prostatic spermidine synthase and spermine synthase with respect to the amine acceptor of the propylamine group was studied. 2. Spermidine synthase could use cadaverine (1,5-diaminopentane) instead of putrescine, but the Km for cadaverine was much greater and the rate with 1mM-cadaverine was only 10% of that with putrescine. 1,3-Diaminopropane was even less active (2% of the rate with putrescine) and no other compound tested (including longer alpha,omega-diamines, spermidine and its homologues and monoacetyl derivatives) was active. 3. Spermine synthase was equally specific. The only compounds tested that showed any activity were 1,8-diamino-octane, sym-homospermidine, sym-norspermidine and N-(3-aminopropyl)-cadaverine, which at 1mM gave rates 2, 17, 3 and 4% of the rate with spermidine respectively. 4. The formation of polyamine derivatives of cadaverine and to a very small extent of 1,3-diaminopropane was confirmed by exposing transformed mouse fibroblasts to these diamines when synthesis of putrescine was prevented by alpha-difluoromethylornithine. Under these conditions the cells accumulated significant amounts of N-(3-aminopropyl)cadaverine and NN'-bis(3-aminopropyl)cadaverine when exposed to cadaverine and small amounts of sym-norspermidine and sym-norspermine when exposed to 1,3-diaminopropane.

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“…Ornithine decarboxylase in hyphae Achlya ornithine decarboxylase exhibited many properties in common with the enzyme from other fungi (Mitchell & Rusch, 1973;Brawley & Ferro, 1979;Aleksijevic et al, 1979;Inderlied et al, 1980) and higher eukaryotes (Janne & Williams-Ashman, 1970, and references therein). Pyrixodal phosphate stimulated enzyme activity in crude extracts and the apparent Km for the cofactor was 0.02 mM.…”

Section: R E S U L T Smentioning

confidence: 98%

Ornithine Decarboxylase Activity and Polyamine Content during Zoospore Germination and Hormone-induced Sexual Differentiation of Achlya ambisexualis

et al. 1982

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The activity of ornithine decarboxylase (EC 4.1 . 1 .17) and the intracellular pools of putrescine and spermidine were determined during zoospore germination and hormoneinduced sexual morphogenesis in Achlya ambisexualis. The specific activity of ornithine decarboxylase increased approximately 6-fold during zoospore germination and outgrowth in an enriched medium. Ornithine decarboxylase activity exhibited a rapid and transient 5 -2-fold increase during hormone-induced differentiation of mycelium cultured in enriched media. In contrast, the enzyme activity did not increase following hormone treatment of mycelium cultured in a minimal medium, yet mycelial differentiation occurred normally. Fluctuations in intracellular concentrations of putrescine and spermidine, in general, correlated with changes in ornithine decarboxylase activity during hormone-induced differentiation and zoospore germination.

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Studies on polyamine biosynthesis in Euglena gracilis

Cited by 26 publications

References 25 publications

Ornithine Decarboxylase and Arginine Decarboxylase Activities in Meristematic Tissues of Tomato and Potato Plants

Ornithine Decarboxylase and Arginine Decarboxylase Activities in Meristematic Tissues of Tomato and Potato Plants

Specificity of mammalian spermidine synthase and spermine synthase

Ornithine Decarboxylase Activity and Polyamine Content during Zoospore Germination and Hormone-induced Sexual Differentiation of Achlya ambisexualis

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