Studies on Penicillium sp. ZDZ1 chitosanase immobilized on chitin by cross-linking reaction

Zeng, Jia; Lian-ying, Zheng

doi:10.1016/s0032-9592(02)00163-2

Cited by 33 publications

(20 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar changes in the kinetic parameters of trypsin after immobilization have been reported by other researchers (Simons, Georgatsos, 1990;Zeng, Zheng, 2002;Kang et al, 2005).…”

Section: Kinetic Parameters K M V Maxsupporting

confidence: 89%

Cotton gauze bandage: a support for protease immobilization for use in biomedical applications

Seabra¹,

Gil²

2007

Rev. Bras. Cienc. Farm.

View full text Add to dashboard Cite

“…Similar changes in the kinetic parameters of trypsin after immobilization have been reported by other researchers (Simons, Georgatsos, 1990;Zeng, Zheng, 2002;Kang et al, 2005).…”

Section: Kinetic Parameters K M V Maxsupporting

confidence: 89%

Cotton gauze bandage: a support for protease immobilization for use in biomedical applications

Seabra¹,

Gil²

2007

Rev. Bras. Cienc. Farm.

View full text Add to dashboard Cite

“…Microbacterium , Aspergillus (Chen et al 2005;Eom and Lee 2003), Gongronella (Wang et al 2008a), Fusarium (Shimosaka et al 1996, Mucor (Struszczyk et al 2009) and Penicillium (Zeng and Zheng 2002) genus. Most chitosanases were inducible in medium containing colloidal chitosan or chitosan as major carbon source.…”

Section: Introductionmentioning

confidence: 99%

Statistical optimization of chitosanase production by Aspergillus sp. QD-2 in submerged fermentation

2011

View full text Add to dashboard Cite

Cultivation of chitosanase production by Aspergillus sp. QD-2 under submerged fermentation was optimized. Factors including (NH 4 ) 2 SO 4 , inoculum size and initial pH were identified by two-level PlackettBurman design (PBD) as significant for chitosanase production. The path of steepest ascent was undertaken to determine the optimal region of three significant factors. To determine the optimal values of the significant variables, Box-Behnken design (BBD) and response surface analysis were employed. The quadratic regression model of producing chitosanase showed that the optimal cultivation conditions ((NH 4 ) 2 SO 4 5.164 g/l, inoculum size 8.204% (v/v) and initial pH 4.074) resulted in the improvement of chitosanase activity (85.816 U/ml) as compared to the initial level (26.515 U/ml) after 72 h of fermentation. The optimal pH and temperature of the crude chitosanase were determined to be 5.6 and 55°C, respectively.

show abstract

“…Chitin, a polysaccharide containing 2-deoxy-2-amino glucose units linked by b -1,4 linkages, is obtained as a by-product of crustaceans, insects, marine crabs, etc. (Zeng and Zheng, 2002) and is much cheaper than agarose.…”

Section: Introductionmentioning

confidence: 99%

Stabilization of penicillin G acylase by immobilization on glutaraldehyde-activated chitosan

Adriano

Filho

Silva

et al. 2005

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-The objective of this work was to study enzyme immobilization on chitosan activated with glutaraldehyde, aiming to produce a cheap biocatalyst. Two different immobilization strategies were studied: one-point and multipoint covalent attachment to the solid matrix. The multipoint covalent attachment derivative had an 82% immobilization yield. It was 4.9-fold more stable than the free enzyme at 50°C and 4.5-fold more stable than soluble enzyme at pH 10.0. The one-point derivative had an 85% immobilization yield. It was 2.7-fold more stable than the free enzyme at 50°C and 3.8-fold more stable than soluble PGA at pH 10.0. Results indicated that chitosan can be loaded with PGA above 330 IU/g. Intraparticle diffusive effects, however, limited hydrolysis of penicillin G catalyzed by those derivatives at 37°C and 25°C. Operational stability assays were performed and the multipoint derivative exhibited a half-life of 40 hours.

show abstract

Studies on Penicillium sp. ZDZ1 chitosanase immobilized on chitin by cross-linking reaction

Cited by 33 publications

References 10 publications

Cotton gauze bandage: a support for protease immobilization for use in biomedical applications

Cotton gauze bandage: a support for protease immobilization for use in biomedical applications

Statistical optimization of chitosanase production by Aspergillus sp. QD-2 in submerged fermentation

Stabilization of penicillin G acylase by immobilization on glutaraldehyde-activated chitosan

Contact Info

Product

Resources

About