Studies on nucleoproteins IV. Preparation of the deoxyribonucleoprotein and fractionation of the deoxyribonucleic acid of wheat germ

Deoxyribonucleic acid of cotton

“…The metho(I use(d for isolating DNA froml cottoi, which (liffers in certain essentials froml that used by Lipshitz andl Chargaff (11) oIn wheat germ, resulted from unsuccessful attempts to apply their metho(l to cotton. The metho(I for cotton wvas applicable also to corn, peanutt, and sunflower.…”

“…In contrast, the DNA composition of higher plants has received relatively little attention (11,14,15,16). This applies particularly to studies, e.g., those on wheat (11,16), in which isolation and purification of the DNA have preceded determination of the constituent purine and pyrimidine bases.…”

“…ANALYTICAL: Hydrolysis of the Na-DNA isolates to the free purine and pyrimidine bases and their chromatographic separation and deternmination followed closely the procedure outlined by Lipshitz and Chargaff (11). Four hydrolysates of each isolate were used for (letermining the bases, which are expressed as moles per 100 gram-atoms of phosphorus.…”

A Plant DNA-Binding Protein That Recognizes 5-Methylcytosine Residues

“…

A novel, 5-methylcytosine-specific, DNA-binding protein, DBP-m, has been identified in nuclear extracts of peas. DBP-m specifically recognizes 5-methylcytosine residues in DNA without appreciable DNA sequence specificity, unlike a mammalian DNA-binding protein (MDBP), which recognizes 5-methylcytosine residues but only in a related family of 14-base-pair sequences.5-Methylcytosine (m5C) is found in the DNA of all studied vertebrates and higher plants (10,12,19,27,28). DNA methylation in higher plants can be distinguished from that in mammals by its higher levels (up to approximately one-third DNA is to help control gene expression (2, 8, 18, 26).

…”

“…5-Methylcytosine (m5C) is found in the DNA of all studied vertebrates and higher plants (10,12,19,27,28). DNA methylation in higher plants can be distinguished from that in mammals by its higher levels (up to approximately one-third DNA is to help control gene expression (2, 8, 18, 26).…”