Studies on Nucleic Acid Metabolism in Suspension Cultures of Cucumis melo L.

Vajranabhaiah, S. N.; Mehta, A. R.

doi:10.1093/oxfordjournals.aob.a085135

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…In melon, the production of calli [1,2,3] and suspension cultures [11,12] have been described. More recently we have reported methods suitable for obtaining callus from protoplasts of melon [9], and the formation of somatic embryos from these calli (in preparation).…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from calli of melon (Cucumis melo L., cv. ?Amarillo Oro?)

Moreno

Garcia-Sogo

Granell

et al. 1985

Plant Cell Tiss Organ Cult

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Callus cultures from cotyledon and hypocotyl explants of a Spanish cultivar of melon ('Amarillo Oro') have been tested for their growth and morphogenic capacity on a series of media with different concentrations of indole-3-acetic acid (IAA) and 6-furfurylaminopurine (kinetin). Melon tissues were able to undergo morphogenesis both via organogenesis and embryogenesis, depending on culture conditions and explant source. Shoot buds were obtained at high rates in cotyledon explants. In response to 1.5 mg/1 IAA and 6.0 mg/1 kinetin, more than 90% of the calli produced well-developed shoots. Hypocotyls failed to form shoots but formed somatic embryos on auxin containing media while cotyledon explants usually gave abundant shoots but only rarely formed embryos. It was possible to maintain organogenic callus lines for at least 12 months under defined conditions. Plants were recovered from adventitious shoots produced both in cotyledon-derived calli and from organogenic cell lines.

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Section: Introductionmentioning

confidence: 99%

Plant regeneration from calli of melon (Cucumis melo L., cv. ?Amarillo Oro?)

Moreno

Garcia-Sogo

Granell

et al. 1985

Plant Cell Tiss Organ Cult

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DNA RNA and protein content of tissue during growth and embryogenesis in wild-carrot suspension cultures

Verma

Dougall

1978

In Vitro

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A highly selected population of cells (clumps from 63 to 125 micron in diameter), obtained by screening 14-day-old stock suspension cultures of wild carrot (Daucus carota L.), was used to initiate cultures in this study. Time-course changes in DNA, RNA and protein were followed when these cultures were grown in the presence or absence of 2.25 muM 2,4-dichlorophenoxyacetic acid (2,4-D). The data show that growth of these cultures, particularly in the early part of the growth curve, is different from that in most other studies reported on suspension cultures initiated without screening. The gross compositional analysis shows that this difference stems from the very high RNA:DNA and protein: DNA ratios of the cellular material used as the inoculum in this study. The presence of 2,4-D in the medium promoted total RNA and protein levels. Correlations were sought between the appearance of embryos in the absence of exogenous 2,4-D and gross compositional differences developing in cultures grown in the presence and absence of 2,4-D. The handling of cultures during inoculation appeared to have led to a substantial loss of DNA. This had, however, little effect on dry weight or protein content of the tissue.

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Regeneration in Vegetable Species

Reynolds¹

1986

Plant Regeneration and Genetic Variability

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Studies on Nucleic Acid Metabolism in Suspension Cultures of Cucumis melo L.

Cited by 4 publications

References 9 publications

Plant regeneration from calli of melon (Cucumis melo L., cv. ?Amarillo Oro?)

Plant regeneration from calli of melon (Cucumis melo L., cv. ?Amarillo Oro?)

DNA RNA and protein content of tissue during growth and embryogenesis in wild-carrot suspension cultures

Regeneration in Vegetable Species

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