Studies on nuclear ribosomes II. Transfer of ribosomes from nucleus to cytoplasm in the early stages of viral infection

Ginzburg-Tietz, Y.; Kaufmann, E.; Traub, A.

doi:10.1016/0014-4827(64)90373-8

Cited by 14 publications

(5 citation statements)

References 15 publications

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“…In the case of the small ribonucleic acid RNA viruses, it appears that the cell nucleus plays little or no direct role in viral replication. However, for a wide variety of viral agents such as tobacco mosaic virus (11,19), encephalomyocarditis virus (3,7,16), and influenza virus (13), the possibility that the nucleus has an important function in RNA replication has been suggested.…”

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confidence: 99%

Involvement of the Host Cell Nuclear Envelope Membranes in the Replication of Japanese Encephalitis Virus

1974

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The distribution of viral ribonucleic acid (RNA) on various cell membrane fractions derived from a porcine kidney cell line infected with Japanese encephalitis virus was investigated. At 40 h postinfection, after virus growth had reached its peak, three viral RNAs, 45S, 27S, and 20S, were associated with the cytoplasmic membranes and intact nuclei. The amount of each RNA associated with the nucleus was two-to fivefold greater than that present with the cytoplasmic membranes. Treatment of washed infected nuclei with 1.0% Triton X-100, which removed the outer nuclear envelope membrane, also removed the viral RNA. When the nucleus was fractionated into nuclear envelope membranes and a large particle fraction which sedimented at 600 x g, nearly all of the viral RNA remained associated with the envelope membranes. The nuclear envelope membranes contained higher viral RNA polymerase activity than the cytoplasmic membranes derived from the same cells. These data suggest that major sites for Japanese encephalitis virus RNA synthesis may be localized on or in very close association with the nuclear envelope membranes.

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confidence: 99%

Involvement of the Host Cell Nuclear Envelope Membranes in the Replication of Japanese Encephalitis Virus

1974

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“…These results would suggest that the host-related intracellular barriers to picornavirus replication limit to the same extent the expression of all the information of the input viral genome. In addition, the finding (6) that the pattern of host ' A preliminary report of this work was presented at the 69th Annual Meeting of the American Society for Microbiology, [4][5][6][7][8][9] May, 1969, Miami Beach, Fla. cell susceptibility to mengovirus differs from the pattern of host cells productively infected by Bovine enterovirus-1 suggests that the hostrelated intracellular barriers to picornavirus replication are quite specific. Since those cellular factors limiting, or stimulating, the replication of certain picornaviruses may also be control agents in normal cellular metabolism, the determination of the basis of picornavirus restriction may result in some understanding of the control of differential gene expression in normal cells.…”

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confidence: 99%

Host-Dependent Restriction of Mengovirus Replication

Wall

Taylor

1969

J Virol

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Mengovirus infection of a restrictive cell line, Maden's bovine kidney (MDBK), results in a virus yield 1,000-fold less than that obtained from productively infected cell lines such as L cells or Ehrlich ascites tumor cells (EAT). Cells of both types of host systems are infected with comparable efficiencies and are completely killed as a consequence of infection. Infective center assays, coupled with the observation of total cell killing, suggest that comparable numbers of cells synthesize viral antigen and release virus in both types of host system. Viral-specific ribonucleic acid (RNA) synthesis is initiated and proceeds in an identical fashion for approximately 4 hr after the infection of MDBK, EAT, or L-cells. At this time, viral RNA synthesis in MDBK ceases, whereas viral RNA synthesis in EAT and L-cells continues at a linear rate. These results indicate that none of the early viral events leading to the initiation of viral-specific RNA synthesis constitutes the primary site of mengovirus restriction in MDBK. Rather it appears that the cessation of viral RNA synthesis in restrictive cells constitutes the primary limiting event. Based on its delayed interaction with mengovirus RNA synthesis, it appears that the host-related restrictive agent is initially compartmentalized and then released as a consequence of infection subsequent to those early events in mengovirus infection leading to the initiation and continued synthesis of viral RNA.

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“…Ginzburg-Tietz, Kaufmann & Traub (1964) reported a similar observation in ascites cells infected in vitro with various viruses. Since they had shown that the enzyme is bound to nuclear ribosomes (Traub, Kaufmann & Ginzburg-Tietz, 1964), and since in virus-infected cells they recovered the extranuclear enzyme in the ribosomal fraction, they concluded that the presence of NAD pyrophosphorylase outside the nucleus was due to passage of ribosomes from the nucleus to the cytoplasm. The experiments reported in Table 2 indicate that nuclei from regenerating liver lose Table 4.…”

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confidence: 99%

Activity of nicotinamide–adenine dinucleotide pyrophosphorylase in liver nuclei. Effects of partial hepatectomy, hepatotoxins and dietary changes

Stirpe

Corte

1968

Biochemical Journal

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1. The activity of NAD pyrophosphorylase is lower in nuclei isolated from regenerating rat liver than in normal nuclei, and this is due to leakage of the enzyme from the nuclei during the isolation. 2. The NAD pyrophosphorylase activity is lower in liver nuclei from newborn rats, and from rats on a protein-free diet, but no leakage occurs in these cases. 3. Poisoning with alpha-amanitin brings about a transient enhancement of NAD pyrophosphorylase activity in mouse liver nuclei. 4. No changes of enzyme activity were observed after 72hr. starvation, administration of actinomycin D or infection with MHV3 virus.

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Studies on nuclear ribosomes II. Transfer of ribosomes from nucleus to cytoplasm in the early stages of viral infection

Cited by 14 publications

References 15 publications

Involvement of the Host Cell Nuclear Envelope Membranes in the Replication of Japanese Encephalitis Virus

Involvement of the Host Cell Nuclear Envelope Membranes in the Replication of Japanese Encephalitis Virus

Host-Dependent Restriction of Mengovirus Replication

Activity of nicotinamide–adenine dinucleotide pyrophosphorylase in liver nuclei. Effects of partial hepatectomy, hepatotoxins and dietary changes

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