Studies on nuclear ribosomes I. Association of DPN-pyrophosphorylase with nuclear ribosomes in normal and neoplastic tissues

Traub, A.; Kaufmann, E.; Ginzburg-Tietz, Y.

doi:10.1016/0014-4827(64)90372-6

Cited by 38 publications

(6 citation statements)

References 18 publications

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“…This conclusion was drawn on the basis of the fact that HeLa cell nuclei purified by a mixture of a nonionic and an anionic detergent contained no appreciable 16s ribosomal RNA. A similar mixture of detergents was used by other authors (6) to extract ribosomes from the nucleus. The ribosomes may have been extracted from the nuclei in Penman's attempts (41) to rid the nuclei of cytoplasmic ribosomes.…”

Section: Discussionmentioning

confidence: 99%

“…Several reports have described the isolation of ribonucleoprotein particles from nuclear fractions. Ribosomes have been liberated from the nuclei by extraction with Tris buffer (1,2), detergent treatments (3)(4)(5)(6) or ultrasound (5,7), and from nucleoli by deoxycholate (8). Although the nuclear fractions frequently contained considerable contamination by cytoplasmic components and whole cells (1)(2)(3)(4)(5), the nuclear ribosomes were, in many cases, functionally different from cytoplasmic ribosomes (1,2,(4)(5)(6)8).…”

Section: Introductionmentioning

confidence: 99%

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Isolation of Two Distinct Classes of Polysomes From a Nuclear Fraction of Rat Liver

Sadowski

Howden

1968

The Journal of Cell Biology

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Isolated rat liver nuclei were washed with Triton-X-100 in the presence of liver cell sap. This treatment liberated a fraction of polysomes which were isolated by differential centrifugation and were designated "outer membrane polysomes." The outer membrane polysomes synthesized protein in vivo. Shortly after injection of orotic acid-' 4 C, the RNA of outer membrane polysomes had a higher specific activity than that of cytoplasmic polysomes. It was postulated that outer membrane polysomes may be an intermediate in the transfer of newly synthesized RNA from the nucleus to the cytoplasm. In other experiments, Triton-washed rat liver nuclei were lysed in the presence of deoxycholate and deoxyribonuclease. A ribonucleoprotein fraction was isolated from the lysate by differential centrifugation. This fraction contained "intranuclear ribosomes," which sedimented like partially degraded polysomes in sucrose gradients. This degradation could be partially prevented if intranuclear ribosomes were purified by sedimentation through heavy sucrose. The resulting pellets were termed "intranuclear polysomes" because they contained some undergraded polysomes. Intranuclear polysomes were highly radioactive after a brief pulse with orotic acid-' 4 C, but did not appear to synthesize protein rapidly in vivo. Intranuclear polysomes may represent the initial stage of assembly of polyribosomes in the nucleus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of Two Distinct Classes of Polysomes From a Nuclear Fraction of Rat Liver

Sadowski

Howden

1968

The Journal of Cell Biology

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“…Ionic detergents, or combinations of nonionic and ionic detergents, have been utilized for the 8 48 B R I E F N O T E S isolation of nuclei from mammalian cells (5,6) . We examined the effects of the ionic detergent sodium dodecyl sulfate and the nonionic detergent Tween-80, used singly or in combination, on sea urchin nuclei .…”

Section: Results and Commentsmentioning

confidence: 99%

Isolation of Nuclei From Sea Urchin Eggs and Embryos

Thaler

Cox

Villee

1969

The Journal of Cell Biology

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“…Isolation of c y t o p l a s m was p e r f o r m e d b y cell h o m o g e n i z a t i o n in h y p o t o n i c buffer c o n t a i n i n g 10 m~ Tris p H 7.4, 10 m~4 KC1, 1.5 m~ MgC12, t m~ D T T (RSB) as previously described (31). C y t o p l a s m f r a c t i o n a t i o n was m a d e b y cent r i f u g a t i o n on discontinuous sucrose g r a d i e n t according to t h e m e t h o d of CALIGUn~I a n d T A~ (4) w i t h t h e following modification.…”

Section: Preparation and Fraetionation O/cytoplasmmentioning

confidence: 99%

Localization of reverse-transcriptase in interferon-treated mouse cells chronically infected with Moloney leukemia virus

Sherman

Eylan

Teitz

1980

Archives of Virology

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Interferon treatment of mouse cells chronically infected with Moloney leukemia virus (3T3/MLV) resulted in 97 per cent inhibition of infective virus release. The intracellular localization and distribution of virus reverse-transcriptase and group specific (gs) antigen were determined in interferon treated and control cells. Cytoplasm of infected cells was fractionated by isopycnic centrifugation on discontinuous sucrose gradients. Fractions were analysed for their chemical composition and characterized by the activity of membranal marker enzymes. The association and levels of viral antigens were determined in each fraction. Fractions enriched with 5' nucleotidase, specific enzyme marker for plasma membrane, were also enriched with viral proteins. In interferon treated cells, intracellular accumulation of viral proteins was specifically localized in the plasma membrane. Threefold increase in reverse-transcriptase level was the maximal accumulation found in purified plasma membranes. Intracellular enzyme levels in interferon treated cells were in accordance with the amount of cell associated infective virus particles. The small accumulation of viral proteins and infective virus particles was not sufficient to account for the great reduction in virus yield observed in the supernatants of the interferon treated cells. A possible role for interferon in modification of plasma membrane associated with virus assembly is postulated.

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Studies on nuclear ribosomes I. Association of DPN-pyrophosphorylase with nuclear ribosomes in normal and neoplastic tissues

Cited by 38 publications

References 18 publications

Isolation of Two Distinct Classes of Polysomes From a Nuclear Fraction of Rat Liver

Isolation of Two Distinct Classes of Polysomes From a Nuclear Fraction of Rat Liver

Isolation of Nuclei From Sea Urchin Eggs and Embryos

Localization of reverse-transcriptase in interferon-treated mouse cells chronically infected with Moloney leukemia virus

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