Studies on nodulation, biochemical analysis and protein profile ofRhizobium isolated from Indigofera species

Kumari, B S; Ram, Mangey; Mallaiah, K. V.

doi:10.21161/mjm.20109

Cited by 9 publications

(4 citation statements)

References 18 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies observed EI values of 3.1 for cowpea rhizobial isolates (Oliveira et al, 2006b) and values of up to 3.5 for fast-growing isolates from pigeonpea (Cajanus cajan) (Fernandes Júnior et al, 2012). Kumari et al (2010) reported the importance of the enzymes urease, protease, amylase, and gelatinase in the formation of nodules, stating that rhizobia that produce these enzymes are considered more effective in nodulation and N fixation.…”

Section: Resultsmentioning

confidence: 97%

Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil

et al. 2018

View full text Add to dashboard Cite

Forage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosanthes capitata cultivated in the soils of a semiarid region of Brazil. A total of 126 bacterial isolates were obtained: 45 isolates from C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. ternatea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose, and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperatures, where isolates that tolerated the highest salt concentration also tolerated the highest temperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. Streptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 16S rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradyrhizobium (4), Leifsonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and genotypically diverse bacteria inhabiting the nodules of the forage legumes C. ternatea and S. capitata represent an important microbial source to protect new biotechnological products and improve forage legumes in semiarid regions.

show abstract

Section: Resultsmentioning

confidence: 97%

Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The EPS produced by the bacteria helps fix atmospheric nitrogen by lowering the high oxygen pressure (Kumari et al, 2009), and it also works to change the form of Rhizobium bacteria from the stick form to the form of nitrogen-fixing known as Bacteroids within the root nodules of host plants (Fraysse et al, 2003).…”

Section: Science Archives (Issn:2582-6697)mentioning

confidence: 99%

Effect of titanium dioxide nanoparticles on the polysaccharides of Rhizobium isolates

Al-Hayani,

Abdulazeez Alhamd

2023

View full text Add to dashboard Cite

This study included testing the production of Rhizobia isolates for external polysaccharides, and the isolates showed mixed results among them, as some isolates outperformed others in the rate of production, and also tested the effect of titanium dioxide on the production of exopolysaccharides for Rhizobia isolates, and the results showed that nanomaterials have an effect There was a significant increase in the rate of production of exopolysaccharide, while the higher the concentration, the rate of production decreased significantly. The size and shape of the nanoparticles were diagnosed using SEM examination and visible spectroscopy to ensure that the nanoparticles of dioxide are within the nanoscales.

show abstract

“…A gelatine test is conducted by inoculating the rhizobia in a gelatine medium. The degradation of the medium is an indication of the presence of a gelatinase enzyme in the rhizobia (Kumari et al, 2010;Tyagi et al, 2017;Qudratullah Oryakhil, 2020). Production of the gelatinase enzyme by rhizobia is among the features that promote nodulation and N 2 fixation (Kumari et al, 2010;Qudratullah Oryakhil, 2020).…”

Section: Characterization Of Soybean-nodulating Rhizobia Morphologica...mentioning

confidence: 99%

“…The degradation of the medium is an indication of the presence of a gelatinase enzyme in the rhizobia (Kumari et al, 2010;Tyagi et al, 2017;Qudratullah Oryakhil, 2020). Production of the gelatinase enzyme by rhizobia is among the features that promote nodulation and N 2 fixation (Kumari et al, 2010;Qudratullah Oryakhil, 2020). Among the products of gelatine hydrolysis by the gelatinase, enzyme are amino acids and peptides.…”

Section: Characterization Of Soybean-nodulating Rhizobia Morphologica...mentioning

confidence: 99%

Soybean-Nodulating Rhizobia: Ecology, Characterization, Diversity, and Growth Promoting Functions

Nakei

Venkataramana

Ndakidemi

2022

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

The worldwide increase in population continues to threaten the sustainability of agricultural systems since agricultural output must be optimized to meet the global rise in food demand. Sub-Saharan Africa (SSA) is among the regions with a fast-growing population but decreasing crop productivity. Pests and diseases, as well as inadequate nitrogen (N) levels in soils, are some of the biggest restrictions to agricultural production in SSA. N is one of the most important plant-limiting elements in agricultural soils, and its deficit is usually remedied by using nitrogenous fertilizers. However, indiscriminate use of these artificial N fertilizers has been linked to environmental pollution calling for alternative N fertilization mechanisms. Soybean (Glycine max) is one of the most important legumes in the world. Several species of rhizobia from the four genera, Bardyrhizobium, Rhizobium, Mesorhizobium, and Ensifer (formerly Sinorhizobium), are observed to effectively fix N with soybean as well as perform various plant-growth promoting (PGP) functions. The efficiency of the symbiosis differs with the type of rhizobia species, soybean cultivar, and biotic factors. Therefore, a complete understanding of the ecology of indigenous soybean-nodulating rhizobia concerning their genetic diversity and the environmental factors associated with their localization and dominance in the soil is important. This review aimed to understand the potential of indigenous soybean-nodulating rhizobia through a synthesis of the literature regarding their characterization using different approaches, genetic diversity, symbiotic effectiveness, as well as their functions in biological N fixation (BNF) and biocontrol of soybean soil-borne pathogens.

show abstract

Studies on nodulation, biochemical analysis and protein profile ofRhizobium isolated from Indigofera species

Cited by 9 publications

References 18 publications

Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil

Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil

Effect of titanium dioxide nanoparticles on the polysaccharides of Rhizobium isolates

Soybean-Nodulating Rhizobia: Ecology, Characterization, Diversity, and Growth Promoting Functions

Contact Info

Product

Resources

About