Studies on nerve cell affinity of biodegradable modified chitosan films

Abstract

Chitosan, a natural polysaccharide that has excellent biocompatibility and biodegradability, can be used as nerve conduit material. The purpose of this work was to study the ability of chitosan and some chitosan-derived materials to facilitate nerve cell attachment, differentiation and growth. The biomaterials studied were chitosan, poly-L-lysine-blended chitosan (CP), collagen-blended chitosan (CC) and albumin-blended chitosan (CA), with collagen control material. Culture of PC12 cells and fetal mouse cerebral cortex (FMCC) cells on these biomaterials was used to evaluate their nerve cell affinity. The composite materials, including CP, CC and CA, had significantly improved nerve cell affinity compared to chitosan, as established by increasing attachment, differentiation and growth of PC12 cells. FMCC cells could also grow better on composite materials than on chitosan. CP exhibited the best nerve cell affinity among these three types of composite material. CP is an even better material in promoting neurite outgrowth than collagen, a substrate that is widely used in tissue engineering, suggesting that CP is a promising candidate material for nerve regeneration.

In vitro and in vivo evaluation of a biodegradable chitosan–PLA composite peripheral nerve guide conduit material

“…Chitosan, an abundant natural biodegradable material, has been proved to be biocompatible with nerve cells and can be used as nerve conduit to facilitate nerve cell attachment, differentiation, and growth, [4][5][6][7] but pure chitosan is brittle and degrades rapidly. [8][9][10] In use as a nerve conduit, it breaks easily, causing lumen collapse and subsequent blockage of nerve regeneration.…”

Effects of a laminin peptide (YIGSR) immobilized on crab‐tendon chitosan tubes on nerve regeneration

“…Several studies have described attempts to develop composites of chitosan and other biomaterials to improve the biocompatibility and biodegradability of chitosan; poly-L-lysine, collagen, albumin, and gelatin. [13][14][15] Cheng et al compared collagen with chitosan, both alone and blended with poly-L-lysine, collagen, and albumin. 13,14 The composite materials significantly improved nerve-cell affinity compared to chitosan alone, as evidenced by improvement in the attachment, differentiation, and growth of PC12 cells.…”

“…[13][14][15] Cheng et al compared collagen with chitosan, both alone and blended with poly-L-lysine, collagen, and albumin. 13,14 The composite materials significantly improved nerve-cell affinity compared to chitosan alone, as evidenced by improvement in the attachment, differentiation, and growth of PC12 cells. The chitosan-poly-L-lysine composites gave rise to the largest differences.…”

Immunophilin FK506 loaded in chitosan guide promotes peripheral nerve regeneration

“…Chitosan, the deacetylated derivative of chitin, is a copolymer of D-glucosamine and N-acetyl-D-glucosamine. With the benefit of its neural cell compatibility (Cheng et al 2003), biodegradation (Freier et al 2005), and neuroprotective effect of the biodegradation products (Jiang et al 2009), chitosan has been used as a candidate material for peripheral nerve regeneration (Chavez-Delgado et al 2005).…”