Studies on mRNA expression of basic fibroblast growth factor in wound healing for wound age determination

Abstract

We investigated the mRNA expression of basic fibroblast growth factor (bFGF) for wound age determination during dermal, cerebral, hepatic and renal wound healing in mice. The bFGF mRNA expression in the injured skin peaked at 1 h and was detected in epidermal cells, fibroblasts, endothelial cells and neutrophils. In the injured cerebrum the expression increased from 1 h and peaked at 48 h. In the intact cerebrum, bFGF was detected exclusively in the endothelial cells, whereas it was also detected in astrocytes during wound healing. Time-dependent expression of bFGF mRNA in skin and cerebrum was considered to be useful for wound age determination. On the other hand it was suggested that bFGF mRNA in astrocytes could be a vital sign of the acute phase. In hepatic and renal injuries, however, bFGF mRNA expression increased slightly in endothelial cells at 24 h, in neutrophils of the liver and in the glomeruli of the kidneys.

Efficacy of three different laser wavelengths for in vitro wound healing

“…The indirect ELISA (34, 35) was performed according to previously published methods (15, 18). Briefly, 100 μl of culture medium was diluted in 100 μl carbonate–bicarbonate buffer (Sigma‐Aldrich C3041, St. Louis, MO, USA) and incubated overnight at 4 °C.…”

“…Two hundred microliters of anti‐mouse IgG (Fab specific) peroxidase‐conjugated antibody (Santa Cruz Biotechnology sc‐2005 200 μg/0.5 ml, Santa Cruz, CA, USA) diluted (1 : 4000) in PBS‐T was used as the secondary antibody while 100 μl of TMB substrate reagent (BD Biosciences #555214, Rockville, MD, USA) was added for colorimetric detection. The orange‐yellow color development was stopped after 30 min with 1 mol/L H 2 SO 4 and the positive wells were read at 450 nm (34, 35).…”

A cytokine axis regulates elastin formation and degradation

“…The potent suppressive effects of bFGF may underlie attenuated/defective elastogenesis observed in wound healing processes in which bFGF levels are increased, such as in dermal, cerebral, hepatic, renal and tendon wound healing (Takamiya et al, 2003). bFGF expressed in these in vivo settings or even in cultured cells may oppose the action of positive effectors of elastogenesis.…”

Current genetic methodologies in the identification of disaster victims and in forensic analysis

“…Real-time PCR results using RNA extracted from various tissues indicated that RNA degradation did not correlate with the post-mortem interval, but, rather, with other parameters, like environmental impact and circumstances of death (Li et al 2004; Heinrich et al 2007). Today, body fluid identification (blood, saliva, semen, vaginal secretion and menstrual blood) relying on the quantification of specific mRNAs is an established technique in many forensic laboratories (Juusola and Ballantyne 2005; Nussbaumer et al 2006; Zubakov et al 2008); other possible applications (reviewed in Bauer 2007) include wound age estimation (Takamiya et al 2003) and determination of the age of biological stains by means of RNA decay analysis (Bauer et al 2003; Anderson et al 2005). Stable RNA markers were ascertained for the identification of tissue specificity in old, degraded samples (Zubakov et al 2008, 2009).…”