Studies on Monotreme Proteins VII. Amino Acid Sequence of Myoglobin From the Platypus, Ornithorhynchus Anatinus

Abstract

Myoglobin isolated from skeletal. muscle of the platypus contains 153 amino acid residues. lhe complete amino acid sequence has been determined following cleavage with cyanogen bromide and further digestion of the four fragments with trypsin, chymotrypsin, pepsin and thermolysin. Sequences of the purified peptides were determined by the dansyl-Edman procedure. The amino acid sequence showed 25 differences from human myoglobin and 24 from kangaroo myoglobin. Amino acid sequences in myoglobins are more conserved than sequences in the exand fi-globin chains, and platypus myoglobin shows a similar number of variations in sequence to kangaroo myoglobin when compared with myoglobin of other species. The date of divergence of the platypus from other mammals was estimated at 102 � 31 million years, based on the number of amino acid differences between species and allowing for multiple mutations during the evolutionary period. This estimate differs widely from the estimate given by similar treatment of the ex- and fi-chain sequences and a constant rate of mutation .of globin chains is not supported.