Studies on model membrane. I. Effects of Ca2+ and antibiotics on permeability of cardiolipin liquid-crystalline vesicles

Saha, Jhinuk; Papahadjopoulos, Demetrios; Wenner, Charles E.

doi:10.1016/0005-2736(70)90160-4

Cited by 36 publications

(3 citation statements)

References 21 publications

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“…The observed effects of sonic irradiation (namely, the formation first of large multilamellar vesicles which are then transformed into smaller unilamellar vesicles) are in agreement with those reported by Papahadjo-poulos andMiller (1967), Chapman, Fluck, Penkett andShipley (1968), and Saha, Papahadjopoulos and Wenner (1970).…”

Section: Discussionsupporting

confidence: 91%

Preparation and characteristics of lipid vesicles

Miyamoto

Stoeckenius

1971

J. Membrain Biol.

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As determined by electron microscopy, lipid sonicated in buffer initially forms large vesicles which may be multilamellar. Prolonged sonication results in a population of vesicles of smaller, but not uniform diameters. These vesicles are bounded by only one bilayer. The lipid suspension can be partially fractionated according to size by column chromatography. A fraction of the eluate has been selected for further study. The weight-average vesicle weight and average radius of gyration are obtained by lightscattering measurements. The volume of buffer enclosed by the vesicles is determined using(14)C- or(3)H-labelled sugars as a marker. These values are in reasonable agreement with the corresponding values calculated from the size distribution of the vesicle fraction obtained by electron microscopy.

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Section: Discussionsupporting

confidence: 91%

Preparation and characteristics of lipid vesicles

Miyamoto

Stoeckenius

1971

J. Membrain Biol.

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“…However, our method was limited to the Ogata's method in that the concentrations, combinations of lipids and dispersion methods were exactly the same. Saha et al [17] stated that micelles of cardiolipin prepared by the vortex mixer in water presented heterogenous multiconcentric, lamellar structures ; while the cardiolipin micelles prepared by our gentle mechanical dispersion method have presented a network structure. It may imply that cardiolipin is more hydrophilic and can take up water molecules more readily and thus more apt to form a trifurcate structure.…”

Section: Discussionmentioning

confidence: 93%

Electron Microscopic Observations of Lipid Antigens With Special Reference to the Wassermann Antigen

Kanemasa

1974

Japanese Journal of Microbiology

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The Wassermann antigen is a lipid micelle, and the essential component of the hapten is cardiolipin. It requires the addition of lecithin and cholesterol as auxiliary lipids. We conducted a study on the role of auxiliary lipids in the formation of the micelle structure using negative staining and observing with the electron microscope. The following results were obtained:(1) Cardiolipin, when dispersed in Mg-NaCl solution, presented an irregular network structure.Lecithin, on the other hand, revealed a regular lamellar structure.Cholesterol, since it is nonpolar, immediately agglutinated and precipitated when suspended in the aqueous system. (2) A combination of cardiolipin and lecithin presented a regular lamellar structure, but no network structure of cardiolipin was observed in the visible field. The combination of cardiolipin and cholesterol revealed cardiolipin surrounding structureless cholesterol with its network structure.The combination of lecithin and cholesterol exhibited a lamellar structure surrounding the cholesterol. (3) A combination of all three revealed. a variegated picture of a lamellar structure formed by cardiolipin and lecithin surrounding the cholesterol as a core or of onionskin-like micelles attached to the above structure.

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“…Huang, 1969;Saha, Papahadjopoulos & Wenner, 1970;Hauser, 1971;Sheetz & Chan, 1972) or lipid mixtures (e.g. Huang, 1969;Saha, Papahadjopoulos & Wenner, 1970;Hauser, 1971;Sheetz & Chan, 1972) or lipid mixtures (e.g.…”

Section: Discussionmentioning

confidence: 99%

Pigment containing lipid vesicles

Ritt

Walz

1976

J. Membrain Biol.

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Vesicles obtained by sonication of chlorophyll a-lecithin mixtures dispersed in anaqueous medium closely resemble the well-characterized vesicles similarly prepared from pure lipids. They are bounded by one spherical lipid bilayer which contains the chlorophyll a. Appropriate conditions for sonication prevent substantial degradation of the membrane constituents. Up to one chlorophyll a molecule per 55 lecithins can be incorporated into membranes. The average Stokes' radius of the vesicles determined by analytical sieve chromatography is 102 +/- 5 A and independent of the chloropyll a content. The membrane is visible in the electron-microscope when the vesicles are treated with osmium tetroxide prior to negative staining. The osmium fixation is, however, not strong enough to allow for a preparation of the vesicles for thin sectioning (dehydration, embedding in epoxide).

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Studies on model membrane. I. Effects of Ca2+ and antibiotics on permeability of cardiolipin liquid-crystalline vesicles

Cited by 36 publications

References 21 publications

Preparation and characteristics of lipid vesicles

Preparation and characteristics of lipid vesicles

Electron Microscopic Observations of Lipid Antigens With Special Reference to the Wassermann Antigen

Pigment containing lipid vesicles

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