Studies on lung surfactant replacement in respiratory distress syndrome. Rapid film formation from binary mixed liposomes

Obladen, Michael; Popp, D.; Schöll, Christoph; Schwarz, Heinz; Jähnig, Fritz

doi:10.1016/0005-2736(83)90296-1

Cited by 53 publications

(19 citation statements)

References 20 publications

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“…CLL(V) dispersion microstructure also showed a general characteristic of large thin-walled liposomes. The microstructural features found for rapidly adsorbing CLL dispersions are consistent with results reported by Obladen et al (28). who studied 'The CLL micrographs in Figure 3 are at a magnification of 17,500, but higher the microstructure of a binah mixture of 'inthetic phospho- ' found microstructural features very ciose in appearance to those in Figure 3 to be associated with rapid adsorption (Nottor RH, Penney DP, Finkelstein JN, Shapiro DL, unpublished data).…”

Section: Discussionsupporting

confidence: 86%

Adsorption of Natural Lung Surfactant and Phospholipid Extracts Related to Tubular Myelin Formation

et al. 1986

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ABSTRACT. The microstructure of aqueous dispersions of pulmonary surfactant lipids extracted from bovine lung lavage was investigated by electron microscopic analysis. Following organic solvent extraction (chloroform/methanol) from natural lung surfactant, the mixed lipids (CLL), with 1% residual protein, were dispersed in water by two techniques, probe sonication at 4" C and mechanical vortexing. Surface pressure-time adsorption isotherms were defined for the CLL dispersions, followed by staining with tannic acid, uranyl acetate, and lead citrate for electron microscopy of microstructure. CLL extract dispersions adsorbed in seconds to surface pressures near 45 dynes/cm (surface tension 25 dynes/cm) at low concentrations 5 0.25 mglml after dispersion by sonication of 4" C and by mechanical vortexing. Ultrastructurally, the CLL dispersions were somewhat heterogeneous, but large thin-walled phospholipid vesicles, both intact and fragmented, predominated. No tubular myelin was formed. By contrast, natural lung surfactant (LS) from bronchoalveolar lavage had characteristic regions of tubular myelin when it adsorbed well at low concentrations (5 0.25 mg phospholipid/ml). When divalent cations were removed from solution with 5 mM EDTA, this distinctive microstructure was not present and natural LS adsorbed less rapidly; higher concentrations of 0.63 mg phospholipid/ml were necessary for maximal adsorption of natural LS without tubular myelin. These results suggest that while tubular myelin is associated with optimal adsorption for natural LS, it is not a required configuration for rapid adsorption facility at low phospholipid concentrations in general. Specifically, for dispersions of surfactant extracts, other microstructures allow adsorption facility equivalent to that of natural LS with tubular myelin. (Pediatr Res 20:97-101,1986) AbbreviationsThe behavior of phospholipid dispersions in an aqueous bulk phase is directly relevant to the RDS of premature neonates which is due to a deficiency of pulmonary surfactant (1, 2). In the mature lung, pulmonary surfactant undergoes a series of structural changes as it is released from the lamellar bodies of type I1 pneumocytes, enters the aqueous alveolar hypophase, and adsorbs to the air-water interface. A number of studies with a variety of models, including alveolar type I1 cells in culture, have demonstrated the distinctive LS microstructure known as tubular myelin when secreted LS is present in an aqueous bulk phase (e.g. References 3-6). This LS microstructure appears to be associated closely with adsorption facility, and surfactant associated apoproteins are considered to play an important role in tubular myelin formation in natural LS (5, 6).The administration of exogenous surfactants to the lungs is a promising approach in neonatal RDS therapy (e.g. References 2, 7-1 1) and may also have applications in other lung disorders such as the adult respiratory distress syndrome (e.g. References 8, 12). For in vivo effectiveness, replacement surfactants must have a solut...

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Section: Discussionsupporting

confidence: 86%

Adsorption of Natural Lung Surfactant and Phospholipid Extracts Related to Tubular Myelin Formation

et al. 1986

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“…This may explain why surfactant PG improves the stability of surfactant PC at low surface tension (22). PG may also affect the adsorption rate, in that the poor adsorption of a liposome suspension prepared from DPPC only, is effectively improved by the inclusion of PG (34). These effects of PG, detected with physical methods, may explain why PG to some extent improves the surface properties of DPPC during a surfactant substitution (21,30).…”

Section: Discussionmentioning

confidence: 99%

Composition and Surface Activity of Normal and Phosphatidylglycerol-Deficient Lung Surfactant

1985

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ABSTRAm. The possibility that pulmonary surfactant, characterized by a phosphatidylglycerol deficiency, as in early fetal life, might have inferior surface properties was evaluated. We obtained this specific surfactant from adult rabbits by withholding glucose and giving them an excess of myoinositol by mouth and intravenously. Controls were given a similar quantity of glucose. The myoinositol resulted in a drastic reduction of surfactant phosphatidylglycerol, from 7.2 to 0.3% of phospholipids, and a corresponding increase in phosphatidylinositol from 4.8 to 11.3%. In addition, the myoinositol treatment increased the myoinosit01 that was disaturated from 18.5 to 27.3% ( p < 0.05).

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“…The other non-DPPC surfactant lipids help in adsorption and dynamic respreading of the surfactant film at the interface [29]. During dynamic respiratory cycles, the unsaturated surfactant lipids are extruded into the alveolar subphase from the pulmonary air-aqueous interface and the interfacial surfactant film behaves like a pure DPPC monolayer [30]. Hence, DPPC monolayers closely simulate the surfactant film at the air-aqueous interface and inhibition of DPPC surface activity is also associated with impairment of lung surfactant function in vivo [31].…”

Section: Discussionmentioning

confidence: 99%

Molecular interactions of cord factor with dipalmitoylphosphatidylcholine monolayers: Implications for lung surfactant dysfunction in pulmonary tuberculosis

Chimote

Banerjee

2008

Colloids and Surfaces B: Biointerfaces

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Studies on lung surfactant replacement in respiratory distress syndrome. Rapid film formation from binary mixed liposomes

Cited by 53 publications

References 20 publications

Adsorption of Natural Lung Surfactant and Phospholipid Extracts Related to Tubular Myelin Formation

Adsorption of Natural Lung Surfactant and Phospholipid Extracts Related to Tubular Myelin Formation

Composition and Surface Activity of Normal and Phosphatidylglycerol-Deficient Lung Surfactant

Molecular interactions of cord factor with dipalmitoylphosphatidylcholine monolayers: Implications for lung surfactant dysfunction in pulmonary tuberculosis

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