Studies on liver chromatin RNA from rats treated with alkylating agents

Itzhaki, Ruth F.; Barker, M.; Billington, R. W.

doi:10.1016/0005-2787(76)90238-0

Cited by 6 publications

(2 citation statements)

References 37 publications

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“…As to the RNA content it has previously been founo that there are two main components of chromatin RNA in liver. One is rapidly synthesized, as shown by its incorporation of injected [3H] orotic acid, and the other stable and of lower molecular weight (24); the NaCl-soluble fraction uualiy contained 72 -88% of the former and 50 -70% of the latter. Polylysine binding: Fig.…”

Section: Solubility Of Chromatin Inmentioning

confidence: 99%

“…tions, to find if co-precipitation of RNA and DNA-protein occurred. It had previously been shown that after mixing polylysine with chromatin in an amount sufficient to neutralise all accessible nucleic acid-phosphates, addition of RNAase caused slow degradation of virtually all the RNA (24). This suggested, by analogy with chromatin DNA, that the RNA is bound ionically to protein and so is inaccessible to polylysine.…”

Section: Solubility Of Chromatin Inmentioning

confidence: 99%

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Fractionation of chromatin by differential solubility in dilute salt

Itzhaki¹,

Hell²,

Birnie³

1978

Nucl Acids Res

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Chromatin prepared from the livers of rats was fractionated on the basis of solubility in dilute NaCl. Neither of the fractions obtained was enriched in newly synthesized DNA. The salt-soluble fraction had a higher protein content (usually up to 50%) relative to the DNA, and contained 72% or more of the rapidly synthesized RNA. This RNA was found to be complexed with the salt-soluble deoxyribonucleoprotein, not merely co-solubilized with it. Also, polylysine-binding studies showed that about 70% or more of the nucleic acid phosphates were accessible as compared to about 40% in the unfractionated chromatin. These properties suggested that the soluble fraction was enriched in activity transcribed chromatin. In contrast molecular hybridization studies showed that the complexity of the DNA and its homology with cDNA transcribed from rat-liver polysomal mRNA were the same as those of DNA from unfractionated chromatin, or from the salt-insoluble fraction. This suggests that the criteria commonly accepted as distinguishing between euchromatin and heterochromatin in vitro are not invariably valid.

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Section: Solubility Of Chromatin Inmentioning

confidence: 99%

Section: Solubility Of Chromatin Inmentioning

confidence: 99%