“…Adequate numbers of lines within each population are needed to insure, on the average, equivalent genetic backgrounds for the populations. Tiyawalee (1972) used such "isopopulations" to test for asso ciations between the marker loci for reaction to crown rust and the quantitative trait, groat-protein percentage. He created two isopopulations of oat lines from each cross of a low protein-rust resistant parant with a high protein-rust susceptible parent.…”