Studies on interaction of ribonucleotides with zinc ferrite nanoparticles using spectroscopic and microscopic techniques

Iqubal, Md. Asif; Sharma, Rachana; Kamaluddin, .

doi:10.1016/j.kijoms.2015.06.001

Cited by 18 publications

(13 citation statements)

References 60 publications

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“…Although several metal oxides have been used in previous oligomerization studies, to the best of our knowledge this important group of binary oxide minerals has not yet been tested. The experimental findings reported here, in relation to the latest use of spinel ferrite for the entrapment of ribonucleotides on its surface [ 46 , 47 ], further sheds light on the role of spinel ferrites during the prebiotic chemistry epoch.…”

Section: Introductionmentioning

confidence: 63%

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Thermal Condensation of Glycine and Alanine on Metal Ferrite Surface: Primitive Peptide Bond Formation Scenario

Iqubal

Sharma

Jheeta³

et al. 2017

Life

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The amino acid condensation reaction on a heterogeneous mineral surface has been regarded as one of the important pathways for peptide bond formation. Keeping this in view, we have studied the oligomerization of the simple amino acids, glycine and alanine, on nickel ferrite (NiFe2O4), cobalt ferrite (CoFe2O4), copper ferrite (CuFe2O4), zinc ferrite (ZnFe2O4), and manganese ferrite (MnFe2O4) nanoparticles surfaces, in the temperature range from 50–120 °C for 1–35 days, without applying any wetting/drying cycles. Among the metal ferrites tested for their catalytic activity, NiFe2O4 produced the highest yield of products by oligomerizing glycine to the trimer level and alanine to the dimer level, whereas MnFe2O4 was the least efficient catalyst, producing the lowest yield of products, as well as shorter oligomers of amino acids under the same set of experimental conditions. It produced primarily diketopiperazine (Ala) with a trace amount of alanine dimer from alanine condensation, while glycine was oligomerized to the dimer level. The trend in product formation is in accordance with the surface area of the minerals used. A temperature as low as 50 °C can even favor peptide bond formation in the present study, which is important in the sense that the condensation process is highly feasible without any sort of localized heat that may originate from volcanoes or hydrothermal vents. However, at a high temperature of 120 °C, anhydrides of glycine and alanine formation are favored, while the optimum temperature for the highest yield of product formation was found to be 90 °C.

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Section: Introductionmentioning

confidence: 63%

“…Some selected techniques of the material’s characterization have been described briefly in this article. Details of the material’s characterization have been reported in our previous work [ 46 , 47 ].…”

Section: Methodsmentioning

confidence: 99%

Thermal Condensation of Glycine and Alanine on Metal Ferrite Surface: Primitive Peptide Bond Formation Scenario

Iqubal

Sharma

Jheeta³

et al. 2017

Life

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“…The FTIR spectra were interpreted based on band assignments for nucleobase, nucleoside, and nucleotides in solution as reported previously in the literature. ,− The bands between ∼1000 and 1180 cm –1 correspond to PO 4 bands of the mononucleotide (Figure ). These bands overlap to some extent with the ribose bands between 960 and 1100 cm –1 .…”

Section: Resultsmentioning

confidence: 99%

Nonenzymatic RNA Oligomerization at the Mineral–Water Interface: An Insight into the Adsorption–Polymerization Relationship

Kaddour

Gerişlioğlu²,

Dalai

et al. 2018

J. Phys. Chem. C

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Nonenzymatic RNA polymerization, a major challenge in understanding the origins of life on early Earth, was previously achieved in the presence of montmorillonite clay and high magnesium concentrations (∼75 mM), but the molecular interactions promoting ribonucleotide oligomerization remain unknown. High adsorption capacity of minerals is generally assumed to favor polymerization efficiency; however, this relationship was never shown. Here we examined the relationship between ribonucleotide adsorption affinity and the role of magnesium in the polymerization catalytic efficiency of minerals. Results showed that adsorption of the activated mononucleotide, 2-methylimadzolide of adenosine monophosphate (2-MeImPA), is approximately 10 times higher on zincite (ZnO) than on montmorillonite clay, but only montmorillonite acts as a catalyst for 2-MeImPA polymerization. In the presence of montmorillonite, oligomers formed even in pure water without any salts present. Attenuated total reflectance FTIR and cross-polarization-magic angle spinning 31 P NMR spectroscopy showed that 2-MeImPA mononucleotides adsorption on ZnO directly involves the phosphate moiety, making it unavailable for polymerization. In contrast, mononucleotides adsorbed on montmorillonite by weak amine interactions leaving the phosphate moiety available for polymerization. Thus, providing a favorable orientation of the monomer, rather than a high adsorption capacity, as well as providing a nanoconfined environment and outer-sphere complex formation with the nucleotide are requisite properties for a mineral to be catalytic to nucleotide polymerization.

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“…From the structure of inosine and cytidine, it is observed that inosine is a purine, while cytidine is a pyrimidine. There have been many previous reports − showing that purine nucleotides had a greater binding affinity to zinc containing nanoparticles than pyrimidines probably due to the large number of electrons in the purine double-rings as compared to single-rings of pyrimidines. Thus, for torula yeast RNA, we speculate that the lack of inosine group could be the reason for its low binding ability with ZnO.…”

Section: Discussionmentioning

confidence: 99%

Zinc Oxide Nanoparticle–Poly I:C RNA Complexes: Implication as Therapeutics against Experimental Melanoma

Ramani¹,

Mudge

Morris

et al. 2017

Mol. Pharmaceutics

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There is current interest in harnessing the combined anticancer and immunological effect of nanoparticles (NPs) and RNA. Here, we evaluate the bioactivity of poly I:C (pIC) RNA, bound to anticancer zinc oxide NP (ZnO-NP) against melanoma. Direct RNA association to unfunctionalized ZnO-NP is shown by observing change in size, zeta potential, and absorption/fluorescence spectra upon complexation. RNA corona was visualized by transmission electron microscopy (TEM) for the first time. Binding constant (K = 1.6-2.8 g L) was determined by modified Stern-Volmer, absorption, and biological surface activity index analysis. The pIC-ZnO-NP complex increased cell death for both human (A375) and mouse (B16F10) cell lines and suppressed tumor cell growth in BALB/C-B16F10 mouse melanoma model. Ex vivo tumor analysis indicated significant molecular activity such as changes in the level of phosphoproteins JNK, Akt, and inflammation markers IL-6 and IFN-γ. High throughput proteomics analysis revealed zinc oxide and poly I:C-specific and combinational patterns that suggested possible utility as an anticancer and immunotherapeutic strategy against melanoma.

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Studies on interaction of ribonucleotides with zinc ferrite nanoparticles using spectroscopic and microscopic techniques

Cited by 18 publications

References 60 publications

Thermal Condensation of Glycine and Alanine on Metal Ferrite Surface: Primitive Peptide Bond Formation Scenario

Thermal Condensation of Glycine and Alanine on Metal Ferrite Surface: Primitive Peptide Bond Formation Scenario

Nonenzymatic RNA Oligomerization at the Mineral–Water Interface: An Insight into the Adsorption–Polymerization Relationship

Zinc Oxide Nanoparticle–Poly I:C RNA Complexes: Implication as Therapeutics against Experimental Melanoma

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