Studies on Inhibitors of Skin Tumor Promotion, XII. Rotenoids from Amorpha fruticosa

Konoshima, Takao; Terada, Hiroki; Kokumai, Midori; Kozuka, Mutsuo; Tokuda, Harukuni; Estes, James R.; Li, Leping; Wang, Hui‐Kang; Lee, Kuo‐Hsiung

doi:10.1021/np50096a006

Cited by 54 publications

(28 citation statements)

References 14 publications

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“…The pods (Brett 1946) and leaves (Cao et al 1996) contain natural insecticides. Chemicals isolated from the leaves of false indigo have demonstrated extremely high anti-tumor promoting activities (Konoshima et al 1993). Because of its spring flower display, false indigo is also used horticulturally (Simpson et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Diversity among North American Accessions of False Indigo (Amorpha fruticosa L.) for Forage and Biomass

DeHaan

Ehlke

Wyse

et al. 2006

Genet Resour Crop Evol

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False indigo (Amorpha fruticosa L.) is a perennial leguminous shrub native to North America. The species could potentially be used for livestock forage, biomass energy, reclamation of degraded environments, or as green manure. Future work with the species will depend upon knowledge of available accessions. Our objectives were to determine (1) the range of diversity among accessions, (2) the correlations among traits across locations, and (3) the distribution of variation among accessions. We studied 21 accessions grown at two locations and a subset of 15 accessions grown at a third location. We measured 47 morphological, agronomic, and phenological traits. All traits were influenced by accession in at least one location (p < 0.05). The mean dry matter (DM) biomass yield of accessions in August ranged from 53 to 1515 g plant À1 and was correlated across locations. False indigo had a high second-year leaf concentration, averaging 660 g kg À1 DM at one location in August. Forage quality of false indigo leaves was high, with average crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) concentrations in July of 205, 226, and 235 g kg À1 , respectively. Accession means of forage quality traits were poorly correlated across locations, indicating that it would be difficult to improve forage quality through selection. Diversity among accessions was often related to geographic origin. The two southern accessions were distinct from all other accessions, indicating that accessions from distant geographic regions will likely contribute valuable diversity to a plant breeding program.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Diversity among North American Accessions of False Indigo (Amorpha fruticosa L.) for Forage and Biomass

DeHaan

Ehlke

Wyse

et al. 2006

Genet Resour Crop Evol

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“…Subsequently, a series of papers on isolation of the same and some new rotenoids followed, usually applying conventional column chromatography on various stationary phases complemented with preparative or semi-preparative HPLC (Konoshima et al, 1993; Terada et al, 1993; Lee et al, 2006a,b; Kim et al, 2011; Wu et al, 2015; Muharini et al, 2017). …”

Section: Phytochemical Constituents Of Amorpha Fruticosa: Isoflavonoimentioning

confidence: 99%

“…As a part of screening studies for cytotoxic agents (anti-tumor-promoters), six North American plants belonging to the Amorpha genus were tested using an in vitro assay (Konoshima et al, 1993). Authors found that A. fruticosa exhibited strong inhibitory effects on Epstein-Barr virus early antigen (EBA-EA) activation induced by 12- O -tetradecanoylphorbol-13-acetate.…”

Section: Pharmacological Activity Of Amorpha Fruticosa: Cytotoxicitymentioning

confidence: 99%

Amorpha fruticosa – A Noxious Invasive Alien Plant in Europe or a Medicinal Plant against Metabolic Disease?

et al. 2017

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Amorpha fruticosa L. (Fabaceae) is a shrub native to North America which has been cultivated mainly for its ornamental features, honey plant value and protective properties against soil erosion. It is registered amongst the most noxious invasive species in Europe. However, a growing body of scientific literature also points to the therapeutic potential of its chemical constituents. Due to the fact that A. fruticosa is an aggressive invasive species, it can provide an abundant and cheap resource of plant chemical constituents which can be utilized for therapeutic purposes. Additionally, exploitation of the biomass for medicinal use might contribute to relieving the destructive impact of this species on natural habitats. The aim of this review is to provide a comprehensive summary and systematize the state-of-the-art in the knowledge of the phytochemical composition and the potential of A. fruticosa in disease treatment and prevention, with especial emphasis on diabetes and metabolic syndrome. Also reviewed are aspects related to potential toxicity of A. fruticosa which has not yet been systematically evaluated in human subjects.

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“…20,21 TSN has been shown to inhibit mouse skin tumor promotion and invasion of cancer cells. 22,23 We have previously shown that TSN inhibited nuclear factor-kB activity and induced internalization and degradation of epidermal growth factor receptor. 24,25 As part of our continuing search for compounds to enhance the cytotoxic activity of 2-DG from natural products, we found that TSN enhanced the cytotoxic activity of 2-DG against various human cancer cell lines.…”

Section: Introductionmentioning

confidence: 99%

The combination of tephrosin with 2-deoxy-D-glucose enhances the cytotoxicity via accelerating ATP depletion and blunting autophagy in human cancer cells

Choi

Lee

2011

Cancer Biology & Therapy

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Abbreviations: AMPK, AMP-activated protein kinase; 2-DG, 2-Deoxy-D-glucose; EF-2, elongation factor-2; eEF-2K, elongation factor-2 kinase; 4EBP1, eukaryotic translation initiation factor 4E binding protein 1; FITC, fluorescein isothiocynate; GFP, green fluorescent protein; LC3, microtubule-associated protein light chain 3B; PARP, poly ADP-ribose polymerase; mTOR, mammalian target of rapamycin; PI, propidium iodide; siRNA, small interfering RNA; TSN, tephrosin 2-Deoxy-D-glucose (2-DG), a synthetic glucose analog that acts as a glycolytic inhibitor, is currently under clinical evaluation for targeting tumor cells. Tephrosin (TSN), a plant rotenoid, is known as an anticancer agent. In this study, we describe that the addition of TSN to 2-DG enhanced the cytotoxic activity of 2-DG against various types of cancer cells by accelerating ATP depletion and blunting autophagy. TSN increased the sensitivity of cancer cells to the cytotoxic effect of 2-DG. The combination of TSN and 2-DG induced acceleration of intracellular ATP depletion and the drastic activation of AMP-activated protein kinase (AMPK), which resulted in the inactivation of the mammalian target of rapamycin (mTOR) pathway. Of particular interest, TSN suppressed 2-DG-induced autophagy, a cell survival process in response to nutrient deprivation. We also showed that TSN inhibited 2-DG-induced activation of elongation factor-2 kinase (eEF-2K), which has been known to regulate 2-DG-induced autophagy. Inhibition of eEF-2K by RNA interference blunted 2-DG-induced autophagy and increased the sensitivity of cancer cells to the cytotoxic effect of 2-DG. The addition of TSN to 2-DG, however, did not enhance the cytotoxic activity of 2-DG by knockdown of eEF-2K, suggesting that inhibition of eEF-2K by tephrsoin could be a critical role in the potentiating effect of TSN on the cytotoxicity of 2-DG. Furthermore, we showed that the blunted autophagy and enhanced cytotoxicity of 2-DG was accompanied by the augmentation of apoptosis. These results show that TSN may be valuable for augmenting the therapeutic efficacy of 2-DG.

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Studies on Inhibitors of Skin Tumor Promotion, XII. Rotenoids from Amorpha fruticosa

Cited by 54 publications

References 14 publications

Evaluation of Diversity among North American Accessions of False Indigo (Amorpha fruticosa L.) for Forage and Biomass

Evaluation of Diversity among North American Accessions of False Indigo (Amorpha fruticosa L.) for Forage and Biomass

Amorpha fruticosa – A Noxious Invasive Alien Plant in Europe or a Medicinal Plant against Metabolic Disease?

The combination of tephrosin with 2-deoxy-D-glucose enhances the cytotoxicity via accelerating ATP depletion and blunting autophagy in human cancer cells

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