Studies on immunogenicity and protective efficacy of DnaJ of Salmonella Typhi against lethal infection by Salmonella Typhimurium in mice

Sagi, Sarada S.K.; Paliwal, Piyush; Bansal, Anju; Mishra, Chittaranjan; Khan, Nadeem; Mustoori, Sai Ram; Ilavazhagan, G.; Sawhney, R. C.; Banerjee, P. K.

doi:10.1016/j.vaccine.2006.06.056

Cited by 18 publications

(8 citation statements)

References 34 publications

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“…For DnaJ, it is known that recombinant DnaJ of Streptococcus pneumoniae and S. enterica serovar Typhimurium were protective against the respective pathogens in mice [43,44], whereas the DnaJ of Flavobacterium columnare induced specific immune response but conferred no protection [45]. In our study, we found that purified recombinant DnaJ was protective against E. tarda challenge in a Japanese flounder model.…”

Section: Discussionmentioning

confidence: 45%

Edwardsiella tarda DnaJ is a virulence-associated molecular chaperone with immunoprotective potential

Dang

Zhang

Sun

2011

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 45%

Edwardsiella tarda DnaJ is a virulence-associated molecular chaperone with immunoprotective potential

Dang

Zhang

Sun

2011

Fish & Shellfish Immunology

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“…35 Previously, we developed recombinant (r) Hsp 60 (GroEL) and rHsp70 (DnaK) from Salmonella enterica serovar Typhi, and we reported that these candidate vaccine molecules stimulate both arms of immunity against S. Typhi and S. typhimurium infection in mice. [36][37][38][39] HSPs share high sequence homology among various spp., and we have previously reported the in vitro and in vivo cross-protective efficacy of rGroEL of S. Typhi against multiple bacterial pathogens. 40 With this background, the present study was designed to evaluate the immunogenicity and protective efficacy of the rIpaB domain of Shigella alone (without any adjuvant) and to investigate the adjuvant effect of rGroEL of S. Typhi when co-administered with the rIpaB domain in mice against infection with S. flexneri, S. boydii and S. sonnei.…”

Section: Introductionmentioning

confidence: 99%

Co-administration of rIpaB domain of Shigella with rGroEL of S. Typhi enhances the immune responses and protective efficacy against Shigella infection

et al. 2015

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Shigella species cause severe bacillary dysentery in humans and are associated with high morbidity and mortality. The Invasion plasmid antigen (IpaB) protein, which is conserved across all Shigella spp., induces macrophage cell death and is required to invade host cells. The present study evaluates the immunogenicity and protective efficacy of the recombinant (r) domain region of IpaB (rIpaB) of S. flexneri. rIpaB was administered either alone or was co-administered with the rGroEL (heat shock protein 60) protein from S. Typhi as an adjuvant in a mouse model of intranasal immunization. The IpaB domain region (37 kDa) of S. flexneri was amplified from an invasion plasmid, cloned, expressed in BL21 Escherichia coli cells and purified. Immunization with the rIpaB domain alone stimulated both humoral and cell-mediated immune responses. Furthermore, robust antibody (IgG, IgA) and T-cell responses were induced when the rIpaB domain was co-administered with rGroEL. Antibody isotyping revealed higher IgG1 and IgG2a antibody titers and increased interferon-gamma (IFN-c) secretion in the co-administered group. Immunization of mice with the rIpaB domain alone protected 60%-70% of the mice from lethal infection by S. flexneri, S. boydii and S. sonnei, whereas co-administration with rGroEL increased the protective efficacy to 80%-85%. Organ burden and histopathological studies also revealed a significant reduction in lung infection in the co-immunized mice compared with mice immunized with the rIpaB domain alone. This study emphasizes that the co-administration of the rIpaB domain and rGroEL protein improves immune responses in mice and increases protective efficacy against Shigella infection. This is also the first report to evaluate the potential of the GroEL (Hsp 60) protein of S. Typhi as an adjuvant molecule, thereby overcoming the need for commercial adjuvants.

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“…The chaperone machinery can help many unfolded proteins with proper folding and mediate the degradation of misfolded proteins (32). Immunization of DnaJ protected 70% of mice against lethal challenge by S. Typhimurium infection, indicating the possible use of DnaJ as a candidate vaccine against typhoid (33).…”

Section: Fig 1 In Vivo Gene Expression At 24 H and 48 H Relative To Tmentioning

confidence: 99%

Identification of Salmonella enterica Serovar Pullorum Antigenic Determinants Expressed In Vivo

Chen

et al. 2013

Infect Immun

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Salmonella enterica serovar Pullorum affecting poultry causes pullorum disease and results in severe economic loss in the poultry industry. Currently, it remains a major threat in countries with poor poultry surveillance and no efficient control measures. As S. Pullorum could induce strong humoral immune responses, we applied an immunoscreening technique, the in vivo-induced antigen technology (IVIAT), to identify immunogenic bacterial proteins expressed or upregulated during S. Pullorum infection. Convalescent-phase sera from chickens infected with S. Pullorum were pooled, adsorbed against antigens expressed in vitro, and used to screen an S. Pullorum genomic expression library. Forty-five proteins were screened out, and their functions were implicated in molecular biosynthesis and degradation, transport, metabolism, regulation, cell wall synthesis and antibiotic resistance, environmental adaptation, or putative functions. In addition, 11 of these 45 genes were assessed for their differential expression by quantitative real-time reverse transcription-PCR (RT-PCR), revealing that 9 of 11 genes were upregulated to different degrees under in vivo conditions, especially the regulator of virulence determinants, phoQ. Then, four in vivo-induced proteins (ShdA, PhoQ, Cse3, and PbpC) were tested for their immunoreactivity in 28 clinical serum samples from chickens infected with S. Pullorum. The rate of detection of antibodies against ShdA reached 82% and was the highest among these proteins. ShdA is a host colonization factor known to be upregulated in vivo and related to the persistence of S. Typhimurium in the intestine. Furthermore, these antigens identified by IVIAT warrant further evaluation for their contributions to pathogenesis, and more potential roles, such as diagnostic, therapeutic, and preventive uses, need to be developed in future studies.

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Studies on immunogenicity and protective efficacy of DnaJ of Salmonella Typhi against lethal infection by Salmonella Typhimurium in mice

Cited by 18 publications

References 34 publications

Edwardsiella tarda DnaJ is a virulence-associated molecular chaperone with immunoprotective potential

Edwardsiella tarda DnaJ is a virulence-associated molecular chaperone with immunoprotective potential

Co-administration of rIpaB domain of Shigella with rGroEL of S. Typhi enhances the immune responses and protective efficacy against Shigella infection

Identification of Salmonella enterica Serovar Pullorum Antigenic Determinants Expressed In Vivo

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