Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus

Indrawati, Isna; Chaicumpa, Wanpen; Setasuban, Prasert; Ruangkunaporn, Yuwaporn

doi:10.1016/0020-7519(91)90096-p

Cited by 26 publications

(11 citation statements)

References 12 publications

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“…Several immunogenic diagnostic polypeptides have been identified for diagnosis of Fasciola spp. and other helminths till date (Ruppel et al 1985;Indrawati et al 1991;Attallah et al 2002;Sarimehmetoglu 2002;Ghosh et al 2005). Efficacy of various stage specific antigens of P. epiclitum was compared by ELISA (Jyoti 2001) and revealed that immature intestinal stage antigen was more immunogenic as compared to metacerial, immature ruminal and adult somatic antigens.…”

Section: Resultsmentioning

confidence: 99%

Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum

et al. 2010

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Polypeptide profile of somatic antigen of Paramphistomum epiclitum (PSAg) and Gastrothylax crumenifer (GSAg) was studied by SDS-PAGE. PSAg and GSAg showed 14 and 19 polypeptides in the range of 14.9-95.5 and 13.7-129.6 kDa with six common polypeptides of mol wt 16.8, 21.8, 23.7, 35.5, 43.4 and 70.8 kDa. P. epiclitum experimentally infected sheep sera were used for identification of specific immuno-dominant peptide in the range of 37-40 kDa against P. epiclitum by western blotting. Hyperimmune sera (HIS) was raised in rabbit against the identified polypeptide, IgG was separated from HIS and an immunoaffinity column was constructed with a binding percentage of 83.74 of IgG with CNBr activated Sepharose 4B. Purification of somatic antigen (PSAg) was done with immunoaffinity chromatography and 37-40 kDa protein antigen was isolated in pure form with recovery percentage of 2.97%. This purified fraction of somatic antigen can be used as a candidate antigen for development of serological assay for early diagnosis of paramphistomosis among livestock.

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Section: Resultsmentioning

confidence: 99%

Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum

et al. 2010

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“…Another study reported a non-protein antigen at 35 kDa as a major diagnostic antigen for that infection. 10 Unfortunately, most serological studies on human paragonimiasis have used the enzyme-linked immunosorbent assay (ELISA) for antibody detection without providing information about the molecular mass of the antigens recognized by antibodies in sera from infected individuals. Although the ELISA format is more convenient than the Western blot, it is not ideal for use with crude native antigen extracts that may contain cross-reactive antigens together with specific diagnostic 7) and Paragonimus westermani antigen (lanes [8][9][10][11][12][13][14] for detecting antibodies to Paragonimus antigens by Western blot.…”

Section: Discussionmentioning

confidence: 99%

“…10 Unfortunately, most serological studies on human paragonimiasis have used the enzyme-linked immunosorbent assay (ELISA) for antibody detection without providing information about the molecular mass of the antigens recognized by antibodies in sera from infected individuals. Although the ELISA format is more convenient than the Western blot, it is not ideal for use with crude native antigen extracts that may contain cross-reactive antigens together with specific diagnostic 7) and Paragonimus westermani antigen (lanes [8][9][10][11][12][13][14] for detecting antibodies to Paragonimus antigens by Western blot. Lanes 1 and 8 tested serum from a suspected P. kellicotti patient (later considered to have not been infected) who did not have antibodies to either antigen; lanes 2 and 9 and 5 and 12 were tested with sera from two proven P. kellicotti patients; lanes 3 and 10 and lanes 4 and 11 tested sera from two proven P. westermani patients; lanes 6 and 13 and lanes 7 and 14 tested sera from two healthy Americans without exposure to Paragonimus.…”

Section: Discussionmentioning

confidence: 99%

Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen

Fischer

Curtis²,

Folk³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. We studied the value of an IgG Western blot (WB) with Paragonimus kellicotti (Pk) antigen for diagnosis of North American paragonimiasis. The test was evaluated with sera from patients with Pk and Paragonimus westermani infections, with control sera from patients with other helminth infections, and sera from healthy Americans. All 11 proven Pk infection sera and two samples from suspected cases that were negative by P. westermani WB at the Centers for Disease Control and Prevention (CDC) contained antibodies to antigens at 34 kDa and at 21/23 kDa. Seven of 7 P. westermani sera contained antibodies to the 34 kDa antigen, but only 2 recognized the 21/23 kDa doublet. No control samples were reactive with these antigens. Antibody reactivity declined after praziquantel treatment. Thus, the P. kellicotti WB appears to be superior to P. westermani WB for diagnosing Pk infections, and it may be useful for assessing responses to treatment.

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“…Once successfully accomplished, however, this antigen holds great promise for use in a highly specific serologic assay. For example, Indrawati et al evaluated the soluble crude antigen of P. heterotremus, an important cause of paragonimiasis in Thailand (67). The soluble crude antigen was separated into three fractions, F1, F2, and F3.…”

Section: Immunodiagnosticsmentioning

confidence: 99%

North American Paragonimiasis (Caused by Paragonimus kellicotti ) in the Context of Global Paragonimiasis

2009

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SUMMARY Paragonimus species are highly evolved parasites with a complex life cycle that involves at least three different hosts, i.e., snails, crustaceans, and mammals. The adult forms of Paragonimus species reside and mate in the lungs of a variety of permissive mammalian hosts, including humans. Although human paragonimiasis is uncommonly encountered in North America, both autochthonous and imported disease may be encountered. Paragonimus kellicotti, the species endemic to North America, is a well-known pathogen in wild and domestic animals. Five patients with North American paragonimiasis have been reported in the recent medical literature. The biologic, clinical, radiologic, and laboratory features of paragonimiasis are reviewed, with emphasis on North American paragonimiasis whenever possible.

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Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus

Cited by 26 publications

References 12 publications

Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum

Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum

Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen

North American Paragonimiasis (Caused by Paragonimus kellicotti ) in the Context of Global Paragonimiasis

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