Studies on hypophyseal isografts in mice. I. Biologic aspects

Boot, L. M.; Röpcke, G.

doi:10.1097/00007890-196701000-00022

Cited by 5 publications

(7 citation statements)

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“…This endogenous MTV is expressed in the C3Hf mice at later age and is linked to the late onset of mammary tumors in this strain (26). The incidence of mammary tumors in this C3Hf strain is dramatically increased in mice which have obtained a hypophyseal isograft (5). The 020 mouse strain seldom develops any mammary tumors.…”

Section: Resultsmentioning

confidence: 95%

“…Another way of inducing mammary tumors in the low-mammary-tumor mouse strains is by implantation of multiple hypophyseal isografts in these mice (18). Prolactin is then continuously released, and the excessive amount of this lactogenic hormone causes high-mammary-tumor frequencies in almost all mouse strains (5,6).…”

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confidence: 99%

“…As a rule all experimental animals were kept in plastic cages (three mice per cage). Hormone treatment was carried out as described previously (5) as follows. Four hypophyseal isografts were implanted, two into each kidney of C3Hf females at an age of 6 to 8 weeks; one hypophysis was implanted into the left kidney of castrated 020 males at the age of 6 to 8 weeks, estrone (0.25 mg/liter) being added to the drinking water throughout the whole lifespan of the mouse, while 3 mg of progesterone was given subcutaneously once every week.…”

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confidence: 99%

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Involvement of mouse mammary tumor virus in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains

Deemter¹,

Nusse²,

Röpcke³

et al. 1978

J Virol

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The involvement of the mouse mammary tumor virus (MTV) in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains was studied by comparing the amounts of MTV RNA and MTV DNA sequences in mammary tumors and other tissues of mice with an without hormonal treatments. The following results were obtained. (i) Mammary tumors which appeared in C3H mice as a result of an infection with MTV contained more MTV DNA compared with noninfected organs; these mammary tumors also contained more MTV RNA than was present in lactating mammary gland cells. (ii) Hormonal stimulation by administration of excessive amounts of prolactin via hypophyseal isografts in C3Hf and O20 mice resulted in an increased expression of MTV RNA in the mammary glands. This elevated level of MTV RNA expression was, however, not maintained in the hormone-induced mammary tumors. (iii) Spontaneous mammary tumors in BALB/c mice contained similar levels of MTV DNA and MTV RNA sequences as were found in other cells of these animals.

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Section: Resultsmentioning

confidence: 95%

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confidence: 99%

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confidence: 99%

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Involvement of mouse mammary tumor virus in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains

Deemter¹,

Nusse²,

Röpcke³

et al. 1978

J Virol

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“…As a rule, all experimental animals were kept in plastic cages (three mice per cage). Hormone treatment was carried out as described previously (Boot and Ropcke, 1966) as follows. Four hypophyseal isografts were implanted, two into each kidney of C3Hf females at an age of 6 to 8 weeks; one hypophysis each was implanted into the left kidney of castrated 0 2 0 and C57BL males at the age of 6 to 8 weeks, estrone (0.25 mgAiter) being added to the drinking water throughout the whole lifespan of the mouse, while 3 mg of progesterone was given subcutaneously once every week.…”

Section: Micementioning

confidence: 99%

Quantification of mouse mammary tumor virus structural proteins in hormone‐induced mammary tumors of low mammary tumor mouse strains

Nusse

Boot

Röpcke

1980

Intl Journal of Cancer

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The expression of the mouse mammary tumor virus (MMTV) in hormone-induced mammary tumors was investigated by means of a radioimmunoassay for two major MMTV proteins, gp52 and p27. MMTV proteins were isolated on lectin affinity- and ion-exchange chromatography columns. The purified viral proteins were electrophoretically homogeneous and retained immunoreactivity after labelling with 125iodine. Standard competition assays showed that group-specific antigenic determinants were reacting. Mammary tumors were induced in three strains of mice with a low natural incidence of mammary tumors, C57BL, O20 and C3Hf, by a combined hormone treatment, consisting of hypophyseal isografts and administration of progesterone and estrone. Mammary tumors and mammary glands of hormone-treated animals were extracted and used for competition radioimmunoassays. In general, the tumorigenic hormone treatment resulted in enhanced amounts of MMTV proteins in the mammary glands, compared to the amounts found in lactating mammary glands of untreated animals. The levels of MMTV proteins in the mammary tumors were lower than in the mammary glands.

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“…However, pituitary isografts and systemic PRL treatment increased the development of spontaneous tumors in mice, and cooperated with chemical carcinogens to induce mammary tumors in the rat (Boot et al, 1962;Welsch and Nagasawa, 1977). When PRL À/À mice were crossed with transgenic mice expressing polyoma middle-T antigen driven by the MMTV promoter (PyVT mice), pituitary transplants decreased tumor latency and increased tumor growth rate (Vomachka et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Prolactin induces ERα-positive and ERα-negative mammary cancer in transgenic mice

et al. 2003

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Studies on hypophyseal isografts in mice. I. Biologic aspects

Cited by 5 publications

References 0 publications

Involvement of mouse mammary tumor virus in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains

Involvement of mouse mammary tumor virus in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains

Quantification of mouse mammary tumor virus structural proteins in hormone‐induced mammary tumors of low mammary tumor mouse strains

Prolactin induces ERα-positive and ERα-negative mammary cancer in transgenic mice

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