Studies on histamine in human urine using a fluorometric method of assay

Gates, John A.; Marsh, E A; Sjoerdsma, Albert

doi:10.1016/0009-8981(62)90089-x

Cited by 98 publications

(17 citation statements)

References 14 publications

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“…It is also true that histamine methyltransferase preparations are relatively crude, but there is no evidence that enzymatic methods overestimate histamine compared with fluorimetric methods, and the reference ranges in normal plasma, whole blood, and urine are not significantly different. In the present study the mean values in plasma, urine, and whole blood were 5 nmol/l, 230 nmol/24h, and 560 nmol/l respectively (Table 4), compared with 5 nrnol/l, 400 nmol/24h, and 490 nrnol/l respectively for fluorimetric methods (Oates et al, 1962;Graham et al, 1968;Lorenz et al, 1972).…”

Section: Discussioncontrasting

confidence: 41%

An Improved Radioenzymatic Assay for Histamine in Human Plasma, Whole Blood, Urine, and Gastric Juice

1979

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SUMMARY A radioenzymatic method suitable for the assay of histamine in human blood, urine, plasma, and gastric juice is described. It differs from earlier methods by use of a histamine methyltransferase preparation from pig brain, of high activity tritiated S-adenosylmethionine, and of a heat precipitation step to reduce the previously noted interference from plasma constituents. The method is simpler than those requiring solvent extraction and concentration of histamine, gives recoveries in the range 80-120 %, and so eliminates the need for internal standardisation. The method is sensitive and precise with coefficients of variation for blood, urine, and plasma of 5 %, 6 %, and 13 % respectively. The mean ± standard deviation for normal human plasma histamine is 5 ± 4 nmol/l, for whole blood 559 ± 193 nmol/l, and for urine 229 ± 128 nmol/24h.

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Section: Discussioncontrasting

confidence: 41%

An Improved Radioenzymatic Assay for Histamine in Human Plasma, Whole Blood, Urine, and Gastric Juice

1979

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“…The range of values obtained for the urine histamine concentration was from 0.024 to 0.038 fig per ml (Mean 0.029, SD 0.0052 or 8% of the mean). O ates and co-workers (8) empha sized that histamine was poorly recovered from the resin columns when it was applied in water or 0.9% saline solution. Inorganic salts of the buffer mixture apparently aid in the complete elution of histamine from the columns.…”

Section: Methods Urine Histamine Measurementmentioning

confidence: 99%

“…S h o r e and co-workers (10) demonstrated that fluorescence intensity was proportional to histamine concentrations between 0.005 and 0.5 /ug per ml when 1% OPT was used. O a tes and co workers (8) advised the use of a 'reverse' blank, a sample carried through the extraction and adduct-forming steps, in which forma tion of the histamine OPT adduct, was prevented by acidification before the addition of OPT. This 'reverse' blank proved to be falsely low in our hands since a reagent blank of 1 N HC1 carried through the procedure gave considerably greater fluorescence.…”

Section: Methods Urine Histamine Measurementmentioning

confidence: 99%

“…Histamine was assayed in urine using an ion exchange method developed by O ates, M arsh and Sjoerdsma (8) combined with N oah and Brand's micromodification (9) of the fluorometric histamine assay method of Shore, Burkhalter and Coiin (10). Urine was voided into bottles containing 20 ml toluene and stored frozen at -20° C to prevent bacterial formation of histamine in the urine.…”

Section: Methods Urine Histamine Measurementmentioning

confidence: 99%

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Histamine in Human Urine

Beall¹

1965

Int Arch Allergy Immunol

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“…of six normal HC1 and were assayed for histamine by the ion-exchange butanol extraction method of Oates, Marsh and Sjoerdsma. 16 The findings were placed on data flow sheets and correlated with other biochemical and physiological changes.…”

mentioning

confidence: 99%

Elevation in Urinary and Blood Histamine Following Clinical Renal Transplantation

1971

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A SUBSTANTIAL amount of evidence has been gathered to suggest that an increase in the intracellular formation and release of histamine is involved in tissue and organ allograft rejection 8,10,12,13,15,19 and that pharmacologic measures to control these processes may lead to a prolongation of allograft survival and to a reduction of antibody formation in experimental situations. 4-7, 11, 14, 18 In 1968, the senior author, with Chang and Hume,9 reported the results of 65 random spot-checks of the 24-hour urinary excretion of histamine in 33 renal transplant patients. The studies were carried out from 7 days to 49 months after transplantation. These determinations were carried out too infrequently per patient and were too widely separated in time to indicate a trend in dynamic functional changes as related to the metabolism of this vaso-active amine. Nonetheless, the levels of urinary excretion of histamine did appear in the majority of patients to be lower than normal. Poorer renal function was associated with lower levels of excretion.In the present study, frequent determinations of both blood and urinary levels of histamine were obtained in a smaller group of renal transplant patients and were concentrated largely in the initial month after transplantation.Submitted for publication June 8, 1970. Supported by a grant from the John A. Hartford Foundation. 381Method of Study Eighty-five blood histamine determinations and 131 24-hour urinary excretions of histamine were obtained in ten patients in the comparatively early period after renal transplantation. Three of the transplants were from related living donors and seven kidneys were cadaveric in origin. Three of the cadaveric kidneys were harvested elsewhere on the basis of cardiac death and had longer periods of shock and warm ischemia and four were harvested at this institution on the basis of neurological death with virtually no warm ischemia and very limited periods of cold ischemia.From five to 30 urinary excretions of histamine studies and from three to 36 blood histamine levels were obtained per patient. Blood histamine levels were determined by the method of Shore, Burkhalter and Cohn.17 Twenty-four hour urine specimens were collected in 10 ml. of six normal HC1 and were assayed for histamine by the ion-exchange butanol extraction method of Oates, Marsh and Sjoerdsma.16 The findings were placed on data flow sheets and correlated with other biochemical and physiological changes.The senior author and associates, in the earlier study, found the normal range for the 24-hour urinary excretion of histamine to be from 30 to 85 uAg. with an average of 49 ,ug. per 24 hours. Studies of 24-hour human urinary excretion of histamine, using this same method of Oates, Marsh and

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Studies on histamine in human urine using a fluorometric method of assay

Cited by 98 publications

References 14 publications

An Improved Radioenzymatic Assay for Histamine in Human Plasma, Whole Blood, Urine, and Gastric Juice

An Improved Radioenzymatic Assay for Histamine in Human Plasma, Whole Blood, Urine, and Gastric Juice

Histamine in Human Urine

Elevation in Urinary and Blood Histamine Following Clinical Renal Transplantation

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