Studies on hemoglobin metabolism. IV. Intrahepatocellular site of the globin-haptoglobin degradation after intravenous administration of hemoglobin-haptoglobin complex to the rats.

Intracellular distribution of haem after uptake by different receptors. Haem-haemopexin and haem-asialo-haemopexin

“…The intracellular route by which haem passes from the plasma membrane to its site of catabolism by haem oxygenase on the endoplasmic reticulum after haemopexin-mediated transport of haem is under study in this laboratory. The lysosomes have been implicated as sites of catabolism of haemoglobin-haptoglobin complexes in the liver (Higa et al, 1981). Since haemoglobin is also a source of haem, an intracellular transport route for haem might exist to move haem from the lysosomes to haem oxygenase, and this route might join that for haem transported by haemopexin.…”

Studies on hemoglobin metabolism. VI. Concentration of intact hemoglobin-haptoglobin in the golgi subcellular fractions of rat liver.

“…*' Supported by Grants no. 244025 (1977, 1978, and 1979 The former was resuspended in 1.15 M sucrose and 10 ml of this suspension was placed beneath a discontinuous sucrose gradient constructed by layering 8 ml of 0.86 M, 7 ml of 0.6 M, and 7 ml of 0.25 M sucrose, each containing 5 mM EDTA, pH 7.5. The centrifugation was performed at 64,000xg for 3 h in Beckman SW-27 rotor to obtain three Golgi fractions (Golgi light (Gi) , Golgi intermediate (Gi) , and Golgi heavy (Gh)), a smooth microsome fraction (residual load zone), and a rough microsome fraction (residual pellet) .…”