Studies on Hantavirus Infection in Small Mammals Captured in Southern and Central Highland Area of Vietnam

Luan, Vu Dinh; Yoshimatsu, Kumiko; Endo, Rika; Taruishi, Midori; Huong, Vo Thi; Dat, Dang Tuan; Tien, Pham Cong; Shimizu, Kenta; Koma, Takaaki; Yasuda, Shumpei P.; Nhi, Le Huu Hanh; Huong, Vû Thi Quê; Arikawa, Jiro

doi:10.1292/jvms.11-0567

Cited by 17 publications

(15 citation statements)

References 24 publications

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“…Our study indicated an overall 8.3% hantavirus seroprevalence among the five rodent species tested from southern Vietnam. This prevalence is consistent with previous reports from Vietnam (approximately 2.8% and 8.6% seropositivity in rodents from the Ho Chi Minh City and northern provinces, respectively) (Truong et al 2009, Luan et al 2012. We found hantavirus antibodies in all rat species with the exception of R. exulans.…”

Section: Discussionsupporting

confidence: 93%

Rodents and Risk in the Mekong Delta of Vietnam: Seroprevalence of Selected Zoonotic Viruses in Rodents and Humans

Cương

Carrique-Mas

Be³

et al. 2015

Vector-Borne and Zoonotic Diseases

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In the Mekong Delta in southern Vietnam, rats are commonly traded in wet markets and sold live for food consumption. We investigated seroprevalence to selected groups of rodent-borne viruses among human populations with high levels of animal exposure and among co-located rodent populations. The indirect fluorescence antibody test (IFAT) was used to determine seropositivity to representative reference strains of hantaviruses (Dobrava virus [DOBV], Seoul virus [SEOV]), cowpox virus, arenaviruses (lymphocytic choriomeningitis virus [LCMV]), flaviviruses (tick-borne encephalitis virus [TBEV]), and rodent parechoviruses (Ljungan virus), using sera from 245 humans living in Dong Thap Province and 275 rodents representing the five common rodent species sold in wet markets and present in peridomestic and farm settings. Combined seropositivity to DOBV and SEOV among the rodents and humans was 6.9% (19/275) and 3.7% (9/245), respectively; 1.1% (3/275) and 4.5% (11/245) to cowpox virus; 5.4% (15/275) and 47.3% (116/245) for TBEV; and exposure to Ljungan virus was 18.8% (46/245) in humans, but 0% in rodents. Very little seroreactivity was observed to LCMV in either rodents (1/275, 0.4%) or humans (2/245, 0.8%). Molecular screening of rodent liver tissues using consensus primers for flaviviruses did not yield any amplicons, whereas molecular screening of rodent lung tissues for hantavirus yielded one hantavirus sequence (SEOV). In summary, these results indicate low to moderate levels of endemic hantavirus circulation, possible circulation of a flavivirus in rodent reservoirs, and the first available data on human exposures to parechoviruses in Vietnam. Although the current evidence suggests only limited exposure of humans to known rodent-borne diseases, further research is warranted to assess public health implications of the rodent trade.

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Section: Discussionsupporting

confidence: 93%

Rodents and Risk in the Mekong Delta of Vietnam: Seroprevalence of Selected Zoonotic Viruses in Rodents and Humans

Cương

Carrique-Mas

Be³

et al. 2015

Vector-Borne and Zoonotic Diseases

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“…antaviruses (family Bunyaviridae) are negative-sense RNA viruses harbored by small mammals and have worldwide distribution (1)(2)(3)(4)(5). Despite global spread, hantavirus strains are confined to the geographic range of the host species, and human infections occur following zoonotic transmission from asymptomatic rodent carriers (6).…”

mentioning

confidence: 99%

Host mTORC1 Signaling Regulates Andes Virus Replication

McNulty

Flint

Nichol

et al. 2013

J Virol

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Hantavirus pulmonary syndrome (HPS) is a severe respiratory disease characterized by pulmonary edema, with fatality rates of 35 to 45%. Disease occurs following infection with pathogenic New World hantaviruses, such as Andes virus (ANDV), which targets lung microvascular endothelial cells. During replication, the virus scavenges 5=-m 7 G caps from cellular mRNA to ensure efficient translation of viral proteins by the host cell cap-dependent translation machinery. In cells, the mammalian target of rapamycin (mTOR) regulates the activity of host cap-dependent translation by integrating amino acid, energy, and oxygen availability signals. Since there is no approved pharmacological treatment for HPS, we investigated whether inhibitors of the mTOR pathway could reduce hantavirus infection. Here, we demonstrate that treatment with the FDA-approved rapamycin analogue temsirolimus (CCI-779) blocks ANDV protein expression and virion release but not entry into primary human microvascular endothelial cells. This effect was specific to viral proteins, as temsirolimus treatment did not block host protein synthesis. We confirmed that temsirolimus targeted host mTOR complex 1 (mTORC1) and not a viral protein, as knockdown of mTORC1 and mTORC1 activators but not mTOR complex 2 components reduced ANDV replication. Additionally, primary fibroblasts from a patient with tuberous sclerosis exhibited increased mTORC1 activity and increased ANDV protein expression, which were blocked following temsirolimus treatment. Finally, we show that ANDV glycoprotein Gn colocalized with mTOR and lysosomes in infected cells. Together, these data demonstrate that mTORC1 signaling regulates ANDV replication and suggest that the hantavirus Gn protein may modulate mTOR and lysosomal signaling during infection, thus bypassing the cellular regulation of translation.

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“…Hantavirus-infected rodents have been reported in Southeast Asian countries including Vietnam, Cambodia, Thailand and Singapore [2,9,12,18,21]. Antibody positivity among humans has also been reported in Asian countries including India, Sri Lanka and Vietnam [3,5,7].…”

Section: Discussionmentioning

confidence: 99%

“…Alignment and phylogenetic analysis with known sequences showed that the viruses belonged to SEOV and formed a cluster with those detected in Vietnam and Singapore (Fig. 2) [9,12,21].…”

Section: Prevalence Of Hantavirus Infection In Rodents Captured In 2005mentioning

confidence: 95%

Epidemiology of Hantavirus Infection in Thousand Islands Regency of Jakarta, Indonesia

Ibrahim

Shimizu

Yoshimatsu

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. Hemorrhagic fever with renal syndrome (HFRS) is a rodent-borne zoonotic disease caused by hantavirus infection. Many HFRS cases have been reported in East Asia and North Europe, while the situation in Southeast Asia remains unclear. In this study, the prevalence of hantavirus infection in rodents and humans in Thousand Islands regency, which is close to the port of Jakarta, one of the largest historic ports in Indonesia, was investigated. A total of 170 rodents were captured in 2005, and 27 (15.9%) of the rodents were antibody-positive against Hantaan virus antigen in an immunofluorescence assay (IFA) and Western blotting. Despite the high prevalence in rodents, human sera collected from 31 patients with fever of unknown origin and 20 healthy volunteers in the islands in 2009 did not show positive reaction to the antigen in IFA. To identify the virus in rodents genetically, a total of 59 rodents were captured in 2009. Sera from the rodents were screened for antibody by ELISA, and lung tissues were subjected to RT-PCR. 20 (33.9%) of the 59 rodents were antibody-positive, and 3 of those 20 rodents were positive for S and M genome segments of hantaviruses. Genetic analysis showed that the viruses belonged to Seoul virus and formed a cluster with those in Vietnam and Singapore. These results suggest that a unique group of Seoul viruses has spread widely in Southeast Asia.

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Studies on Hantavirus Infection in Small Mammals Captured in Southern and Central Highland Area of Vietnam

Cited by 17 publications

References 24 publications

Rodents and Risk in the Mekong Delta of Vietnam: Seroprevalence of Selected Zoonotic Viruses in Rodents and Humans

Rodents and Risk in the Mekong Delta of Vietnam: Seroprevalence of Selected Zoonotic Viruses in Rodents and Humans

Host mTORC1 Signaling Regulates Andes Virus Replication

Epidemiology of Hantavirus Infection in Thousand Islands Regency of Jakarta, Indonesia

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