STUDIES ON Gerbera jamesonii Bolus CV. AURANTIACA, UNDER MICROPROPAGATION CONDITION

Abstract

during 2007 and 2008 years. It was intended to find out the most suitable treatments for propagation of Gerbera jamesonii Bolus cv Aurantiaca by using the tissue culture technique. So, the study was done using explants from juvenile leaves and seeds of the plant. The results emphasized that using 30% Clorox for 15 min and 30% Clorox for 10-20 min, respectively for sterilization of explants gave the best result of (100%) survival and free of contamination of leaf and seeds explants. BA at 0.5 mg/l and 1.0 or 2.0 mg/l NAA were the best concentrations for callus formation from leaves. Furthermore, the use of BA on plantlet formation of seeds was not significant when BA was used at either 0.0 or 0.5 mg/l with the different concentrations of NAA and 1.0 mg/l BA plus either 2.0 or 4.0 mg/l NAA. Leaves explant was better than seeds for number of shoots when cultured on the multiplication medium. MS medium plus 1.0 mg/l Kin and 0.5 mg/l NAA was mor the most appropriate for shoot formation from leafs callus. MS medium plus 2.0 mg/l Kin was favoured for number of shoot from seeds. The shoots of Gerbera jamesonii Bolus cv Aurantiaca were successfully rooted when cultured in MS medium supplemented with 1.5 mg/l IBA. Plantlets after root development exhibited 100% survival in plastic pots filled with peat moss and sand at a ratio of 1:1, 2:1 and 3:1 (by volume).

Propagation of Dracaena Umbraculifera Plant by Tissue Culture Technique

“…Warakagoda et al (2007) revealed that 25% Clorox for 20 min was the best surface sterilization procedure for axillary buds of sugarcane. El-Shamy et al (2009) on Gerbera jamesonii emphasized that using 30% Clorox for 15 min and 30% Clorox for 10-20 min, respectively for sterilization of explants gave the best result of (100%) survival and free of contamination of leaf and seeds explants. Omira et al (2011) found that in order to optimize the establishment of in vitro cultures of Anthurium andraeamum shoot tips from adult greenhouse plants, should be surface sterilized by Clorox solution.…”

“…While IBA at 0.1ppm gave the lowest values (1.29 roots and 3.32 cm, respectively). El-Shamy et al (2009) found that shoots of Gerbera jamesonii cv. aurantiaca were successfully rooted when cultured in MS medium supplemented with 1.5 IBA.…”