Studies on frost hardiness in Chlorella ellipsoidea. V. The role of glucose and related compounds

Sadakane, Haruo; Kabata, Kiyotaka; Ishibashi, Kazuko; Watanabe, Tadao; Hatano, Shoji

doi:10.1016/0098-8472(80)90026-x

Cited by 12 publications

(10 citation statements)

References 18 publications

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“…Compared to IAM C-27, NJ-7 fully retained the regulation of unsaturation degree of fatty acids by temperature, while NJ-18 retained the regulation only at temperatures above 15uC. The hardening process of C. vulgaris IAM C-27 involves an accumulation of sucrose (Sadakane et al 1980). In the cold, C. vulgaris ATCC 11468 accumulates raffinose in addition to sucrose (Salerno & Pontis 1989).…”

Section: Effects Of Pre-cultivation Temperature On Antifreeze Capabilitymentioning

confidence: 99%

“…During the hardening process, sucrose (Sadakane et al 1980), soluble proteins (Yoshimoto et al 1991) and unsaturated fatty acid contents (Joh et al 1993) change significantly. Two hardening-specific proteins, HIC6 and HIC12, identified in this strain (Honjoh et al 1995) acted as cryoprotectants and remained soluble even after boiling, showing high hydrophilicity and heat stability (Honjoh et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Alternative Cold Response Modes in Chlorella (Chlorophyta, Trebouxiophyceae) from Antarctica

Hu¹,

Li²,

Xu³

2008

Phycologia

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Chlorella was known to show enhanced antifreeze capability after cold hardening. We isolated Chlorella strains NJ-7 and NJ-18, which display alternative cold response modes from rock surfaces in Antarctica. On the basis of 18S ribosomal (rRNA) sequences, NJ-7 is an Antarctic type of Chlorella vulgaris; NJ-18 is also a 'true' Chlorella species but differs from any previously reported species in structure. NJ-7 partially retained the enhancing effects of low temperature cultivation on freeze tolerance, which correlates with an increase of C18:3-fatty acid content and upregulation of two antifreeze protein genes. NJ-18, however, showed stable freeze tolerance regardless of the precultivation temperature. We propose that cold response modes vary widely in Chlorella and that the adaptation of C. vulgaris to Antarctica may serve as a model system for the evolution of antifreeze mechanisms in a single species of photosynthetic microorganism.

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Section: Effects Of Pre-cultivation Temperature On Antifreeze Capabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alternative Cold Response Modes in Chlorella (Chlorophyta, Trebouxiophyceae) from Antarctica

Hu¹,

Li²,

Xu³

2008

Phycologia

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“…2520 from the Institute of Low Temperature Science. Recently, Hatano et al (1976) and Sadakane et al (1980) examined the effect of several antimetabolites on the process of hardening at 3OC for 4 days in Chlorella. They showed that cycloheximide (CHI) completely suppressed the increase in freezing tolerance during hardening.…”

Section: Introductionmentioning

confidence: 98%

Effect of cycloheximide on the freezing tolerance and ultrastructure of cortical parenchyma cells from mulberry twigs

Niki¹,

Sakai²

1983

Can. J. Bot.

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Experiments were performed using cycloheximide (CHI) on cortical parenchyma cells from mulberry twigs (Morus bombyciz cv. Gorogi) to understand the relationship between the sequence of cytological changes suggesting replacement of the plasmamembrane and the rapid increase in freezing tolerance. Cortical cells from twigs collected on October 16 and 27, which were hardy to −10 and −15 °C, respectively, were still alive after cooling to −70 °C if they had been exposed to −3 °C for 10 to 12 days. In these hardened cells, appearance of microvesicles (MVs) in the peripheral cytoplasm and fusion of these MVs with plasmamembrane were characteristically observed. Neither cytological change nor increase of freezing tolerance was observed in cells treated by CHI before their exposure to −3 °C for 10 to 12 days. However, the suppression of such changes by CHI treatment was effective only in cells which were hardy at −10 to −15 °C and not in the cells of twigs collected on November 4, which were hardy to −20 °C. Consequently, synthesis of some functional proteins during hardening was required for the further development of freezing tolerance, and synthesis had been accomplished before the cells became hardy to −20 °C. Ultrastructural changes brought about by CHI treatment suggest that synthesis of some functional proteins related to the presumed changes of plasmamembrane occurs during hardening.

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“…2D). CHI has been reported to inhibit completely the development of frost hardiness (17). These electron micrographs indicate that the production of lipid body may be involved in the development of frost hardiness.…”

Section: Identification Of Nonpolar Lipid Increased During Hardeningmentioning

confidence: 83%

Accumulation of Free Fatty Acids during Hardening of Chlorella ellipsoidea

Hatano¹,

Kabata²,

Yoshimoto³

et al. 1982

Plant Physiol.

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Chlorela ellipsoidea Gerneck (IAM C-27) (4,9,18,22). However, little attention has been given to any shift of lipid metabolism leading to the membrane changes.Previous studies have shown that the major site of lipid synthesis in Chlorella ellipsoidea shifts from chloroplasts to a cytoplasmic system during hardening (6), and an unknown nonpolar lipid greatly increases with an increase in frost hardiness (10). Electron micrographs of hardened cells showed that a number of lipid bodies appear along plasma membrane during hardening (5).The present results refer to studies on the identification of the unknown nonpolar lipid and its possible involvement in the appearance of lipid bodies and in the development of frost hardiness. A preliminary account of portions of this study has been presented elsewhere (5).

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Studies on frost hardiness in Chlorella ellipsoidea. V. The role of glucose and related compounds

Cited by 12 publications

References 18 publications

Alternative Cold Response Modes in Chlorella (Chlorophyta, Trebouxiophyceae) from Antarctica

Alternative Cold Response Modes in Chlorella (Chlorophyta, Trebouxiophyceae) from Antarctica

Effect of cycloheximide on the freezing tolerance and ultrastructure of cortical parenchyma cells from mulberry twigs

Accumulation of Free Fatty Acids during Hardening of Chlorella ellipsoidea

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