Studies on Encapsulation of Bovine Serum Albumin, Lysozyme and Insulin Through Coaxial Electrospinning

Raheja, Anant; Agarwal, Anuja; Muthuvijayan, Vignesh; Chandra, T.S.; Natarajan, T. S.

doi:10.1166/jbt.2013.1121

Cited by 11 publications

(9 citation statements)

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“…10 Among these strategies, coaxial electrospun offers better drug stability, more complete drug encapsulation, and tighter control of release kinetics. Different kinds of drugs, [11][12][13][14][15][16][17][18] proteins, [19][20][21][22][23][24][25][26] glucocorticoids, 18 vitamins, 27 and genes 28 29 have been encapsulated in fibers by coaxial electrospinning for various biomedicine applications. In the field of blood-compatible materials, for example, Su et al fabricated core-shell poly(L-lactide-coepsilon-caprolactone) nanofibers with heparin incorporated by coaxial electrospinning.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Dipyridamole/Polyurethane Core–Shell Nanofibers by Coaxial Electrospinning for Controlled-Release Antiplatelet Application

Qin¹,

Liu²,

Zhao³

et al. 2016

j nanosci nanotechnol

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To improve the hemocompatibility of blood-compatible materials, antiplatelet drug dipyridamole (DIP) was encapsulated in polyurethane (PU) to form core-shell nanofibers by coaxial electrospinning method. PU solution and DIP/polycaprolactone compound solution served as shell and core fluid, respectively. Uniform fibers without beads, were fabricated and a clear core-shell structure was generated. The diameter of nanofibers and core structure increased with an increase in the flow rate of the core solution. X-Ray diffraction and differential scanning calorimeter showed that the whole system formed solid dispersion and DIP was dispersed in polymer matrix in an amorphous state. In vitro drug release test demonstrated that releasing curve had two phases consisting of initial burst release and sustained release governed by diffusion mechanism. A more flat curve occurred when the diameter of PU shell increased. In this case, the PU shell served as a barrier restricted DIP release from core drug reservoir. In vitro platelet adhesion test demonstrated that the DIP had maintained its antiplatelet effect after coaxial electrospinning process and could present distinguished antiplatelet activity after 7th and 30th days. Finally, DIP loaded core-shell nanofiber mats with good hemocompatibility, were conveniently fabricated by coaxial electrospinning, which has great potential in the field of tissue engineering vascular graft.

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Section: Introductionmentioning

confidence: 99%

Preparation of Dipyridamole/Polyurethane Core–Shell Nanofibers by Coaxial Electrospinning for Controlled-Release Antiplatelet Application

Qin¹,

Liu²,

Zhao³

et al. 2016

j nanosci nanotechnol

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“…The solution was stirred at room temperature for 3 h; added with 0.01% stack sodium nitrate, which was used as a preservative; and then placed in the refrigerator at 4°C for 12 h to fully hydrate. [19][20][21]…”

Section: Preparation Of Microemulsion With Different Protein Concentrationsmentioning

confidence: 99%

Water-in-oil microemulsion: effect of Desmodium intortum protein isolate–emulsifier interaction, and its stability

Zhang

Liu

Yan

et al. 2021

International Journal of Food Properties

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Water-in-oil (W/O) microemulsions exhibit delivery matrix and exhibit poor lipid solubility. Therefore, increasing the stability of W/O microemulsions is necessary to broaden their applications. In our experiment, a protein microemulsion system was constructed and its stability was investigated. The effects of surfactants, co-surfactants, quality ratio of them (Km) and hydrophilic-lipophilic balance (HLB) values were determined by using the pseudoternary phase diagrams and the area of microemulsions (MEs) as the indexes. The results showed that MEs' stability was the best when the limonene was used as oil phase, Tween 20 + span 80 as the surfactant, and anhydrous alcohol as co-surfactant, if and only if HLB = 6.0 and Km = 3:1. Under this condition, the area of micro-emulsion was the largest, and the quality of microemulsion was better when the mass ratio of oil phase and emulsifier was 7:5.The protein microemulsion was a water-in-oil type, with a particle size of 1.41 ± 0.03 μm, containing 1127.37 ± 30.87 μg/g of proteins. Moreover, the temperature, light, and sample observation tests showed that the solution's protein retention rate was lower than those in the microemulsion. Low-concentration NaCl, less than 9% of glucose and maltose exerted no significant influence on the protein microemulsion stability.

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“…The separation and purification of proteins have become one of important requisites in present pharmaceutical and biomedical industries. − Lysozyme ( N -acetylmuramide glycanhydrolase), as a very small enzyme (14.4 kDa), has been widely used in industrial applications, such as a food additive, cell disrupting agent, antibacterial agent, and anticancer agents. − Because of its valuable properties, separation and purification of lysozyme have attracted great attention. Highly efficient, economical, and simple techniques have been further studied to get the highly purified lysozyme .…”

Section: Introductionmentioning

confidence: 99%

Ultrafine Silk-Derived Nanofibrous Membranes Exhibiting Effective Lysozyme Adsorption

Dai

et al. 2017

ACS Sustainable Chem. Eng.

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Protein separation materials that are both selective and effective could have wide applications in fields of bioengineering and pharmaceutical industry. However, preparation of such materials has proven to be extremely challenging. Herein, we present a scalable methodology to prepare carboxyl group functionalized nanofibrous membranes (SFNM) by combining sustainable silk and electrospinning. The naturally abundant silk is thus reconstructed into nanofibrous membranes with tunable surface functions. The resultant SFNMs exhibit integrated properties of ultrathin fiber diameter (125 nm), larger surface area (14 m2 g–1), high porosity, superhydrophilicity, and negatively charged fiber surface, which can reversibly adsorb lysozyme with a robust capacity of 710 mg g–1 and high durability, matching well with the requirements for purifying protein solutions. The fabrication of such fascinating materials may provide new insights into the design and development of multifunctional separation membranes for various applications.

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Studies on Encapsulation of Bovine Serum Albumin, Lysozyme and Insulin Through Coaxial Electrospinning

Cited by 11 publications

References 0 publications

Preparation of Dipyridamole/Polyurethane Core–Shell Nanofibers by Coaxial Electrospinning for Controlled-Release Antiplatelet Application

Preparation of Dipyridamole/Polyurethane Core–Shell Nanofibers by Coaxial Electrospinning for Controlled-Release Antiplatelet Application

Water-in-oil microemulsion: effect of Desmodium intortum protein isolate–emulsifier interaction, and its stability

Ultrafine Silk-Derived Nanofibrous Membranes Exhibiting Effective Lysozyme Adsorption

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