Studies on encapsidated viroid-like RNA I. Characterization of velvet tobacco mottle virus

SUMMARYProtoplasts from Nicotiana clevelandii were infected with velvet tobacco mottle virus (VTMoV) using inocula containing 2 ~tg/ml poly-L-ornithine (PLO), 8 ~tg/ml VTMoV and 0.02 M-potassium citrate pH 5.0. Typically about 47 % of the inoculated protoplasts become infected. The amount of virus particles bound to inoculated protoplasts was increased by a decrease in pH, an increase in PLO concentration or an increase in virus concentration. The one-step growth curve of VTMoV in N. clevelandii protoplasts showed that, although large amounts of virus were synthesized, the multiplication was slower than that published for other viruses. Alpha-amanitin (50 ~tg/ml) had no effect on the rate of accumulation of virus coat protein or on the synthesis of either RNA species of VTMoV when added to cultures immediately after inoculation.

“…B. Francki. Viruses were isolated and purified from glasshousegrown N. clevelandii infected with VTMoV as described by Randles et al (1981).…”

Section: Methodsmentioning

confidence: 99%

Multiplication of Velvet Tobacco Mottle Virus in Nicotiana clevelandii Protoplasts Is Resistant to -Amanitin

Wu¹,

Lu²,

Tien³

1986

“…1). It sedimented ahead of the 366 nucleotide circular ssRNA-2 of velvet tobacco mottle virus (VTMoV; Randles et al, 1981 ;Haseloff & Symons, 1982) The density range of the gradient is shown by the solid line. FDD-DNA, as shown by PAGE analysis, necessitating further purification.…”

Section: Preparation Of Fdd-dnamentioning

confidence: 99%

Small Circular Single-stranded DNA Associated with Foliar Decay Disease of Coconut Palm in Vanuatu

Randles¹,

Hanold²,

Julia³

1987

SUMMARYThe single-stranded DNA previously reported to be associated with foliar decay disease of coconut palm appears to be predominantly circular, on the basis of its behaviour in two-dimensional polyacrylamide gel electrophoresis, electron microscopy, and its resistance to end-labelling following treatment with alkaline phosphatase and polynucleotide kinase. The DNA sedimented at between 12S and 15S and had a contour length for the circular molecules consistent with the predominant DNA having a molecular weight of about 0.43 × 106, and comprising approximately 1300 nucleotides. These properties differ from the genomic DNAs of known plant viruses.

“…These RNA-2 molecules are similar in size, shape and physicochemical properties to viroids (Diener, 1979;Randles et al, 1982). However, unlike viroids, which replicate independently and cause diseases in plants (Diener, 1979), these RNA-2 molecules do not appear to be capable of autonomous replication in plants and infect them only in association with the RNA-1 molecules (Gould et al, 1981;. In Australia, Gould et al (1981) reported that in two of these novel viruses, solanum nodiflorum mottle (SNMV) and velvet tobacco mottle (VTMoV), both the RNA-1 and RNA-2 were necessary for infectivity and the production of virus particles.…”

Section: Introductionmentioning

confidence: 99%

“…wt. about 1.2 x l0 s Gould & Hatta, 1981 ;Tien-Po et al, 1981;Francki et al, 1983b). These RNA-2 molecules are similar in size, shape and physicochemical properties to viroids (Diener, 1979;Randles et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

“…In Australia, Gould et al (1981) reported that in two of these novel viruses, solanum nodiflorum mottle (SNMV) and velvet tobacco mottle (VTMoV), both the RNA-1 and RNA-2 were necessary for infectivity and the production of virus particles. Moreover, although SNMV and VTMoV are related serologically and share considerable nucleotide sequence homology in their RNA molecules (Gould & Hatta, 1981: Haseloff & Symons, 1982, no infectivity occurred when either the RNA-1 or RNA-2 species of VTMoV was substituted for the similar species occurring in SNMV particles (Gould et al, 1981). In contrast, the RNA-2 of a third such virus, lucerne transient streak (LTSV), was found to behave as a satellite RNA and was not necessary either for the replication of LTSV RNA-1 or for the production of virus particles .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Satellite Nature of the Viroid-like RNA-2 of Solanum Nodiflorum Mottle Virus and the Ability of Other Plant Viruses to Support the Replication of Viroid-like RNA Molecules

Jones¹,

Mayo²

1984

SUMMARYThe stock culture of solanum nodiflorum mottle virus (SNMV) was freed from RNA-2 by passage through single lesions induced in inoculated leaves of Nicotiana debneyi by dilute inocula of partially purified SNMV RNA-1. This isolate (S1) was indistinguishable serologically and in symptomatology from the stock culture of SNMV. Using a sensitive bioassay for detecting RNA-2, no SNMV RNA-2 was found in RNA from particles of the S1 isolate purified after three successive passages in Nicotiana clevelandii. Addition of SNMV RNA-2 (which is not infective alone) had little or no effect on the infectivity of S1 RNA but restored the RNA content of particles to that found in the stock culture (RNA-1 and RNA-2). However, S1 did not support the replication of the RNA-2 from lucerne transient streak virus (LTSV). The possible interaction of the RNA-2 species from LTSV and SNMV with the genomes of other plant viruses was tested by mixing the RNA-2 species with nucleic acid from 11 other viruses. The survival of RNA-2 activity on inoculated leaves for up to 15 days complicated the interpretation of the results of these tests but a small amount of LTSV RNA-2 and SNMV RNA-2 was obtained only from particles of turnip rosette virus purified from plants inoculated with the relevant RNA mixtures. These and other data suggest that these RNA-2 molecules with physicochemical properties similar to viroids found in particles of some sobemoviruses are satellite RNA species whose multiplication is assisted by some, but not all, of the viruses in this group.