Studies on effect of different seasons on expression of HSP70 and HSP90 gene in sperm of Tharparkar bull semen

Rajoriya, J. S.; Prasad, J.K.; Ghosh, Subrata; Perumal, P.; Kumar, Anuj; Kaushal, S.; Ramteke, Suman

doi:10.1016/s2305-0500(14)60025-7

Cited by 12 publications

(10 citation statements)

References 25 publications

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“…The high ambient temperature increased the scrotal temperature in males and consequently a decline in semen quality (Taylor and Bogart 1988). Production and reproduction are impaired as a result of drastic changes in biological functions caused by heat stress (Marai et al, 1995).Seminal plasma, which is a complex mixture of secretions contain factors called heat shock proteins family which is one of the intrinsic molecular mechanisms in the cell protecting it from deleterious effects of heat and other stress (Pockley 2001;Rajoriya et al, 2014). The best known of the HSP families is the HSP 70 family (Rynkowska et al, 2011) so HSP70, located in reproductive tissues, has critical roles in many events.…”

Section: Introductionmentioning

confidence: 99%

Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons

Gafer¹,

El-Rahman²,

Rawash³

2015

AJVS

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Objectives of current study were to identify single nucleotide polymorphisms (SNP) in heat shock protein70 (HSP70) promoter gene of bulls (cattle and buffalo) and its association with semen quality traits and some enzymatic, hormonal and lipid parameters during winter and summer seasons. Semen samples of 37 animals (Cattle n=21 and buffalo n=16), were used in the study. Biochemical Parameter and hormonal profile were estimated. Qualities of some of fresh and frozen-thaw semen were evaluated. PCR-SSCP was used to detect SNPs and different patterns were sequenced. The study demonstrate all the measured biochemical parameters and hormonal profile of cattle and buffalo were significantly different (P<0.05) in winter as compared to summer. Results of PCR-SSCP and sequencing detected a new synonymous SNP 812 T>C transition was the same in both species and could clear two genotypes TT and TC. Association analysis revealed significance difference (P<0.001) with all the fresh and some of frozen-thawed semen traits in cattle population and T allele seemed to be the favorable allele. Regarding to buffalo population, different genotypes has significant difference in term of fresh motility, frozen motility at 1h and viability index. The results of association between biochemical and hormonal parameters there were no significance except for Aspartate-aminotransferase (AST) in cattle population and T 3 level in buffalo population and C allele appeared to be the favorable allele. This the first study concerned with the association of HSP70 polymorphisms and semen quality, in winter and summer seasons in buffalo population. Conclusion: High temperature has negative impact on semen quality. Biochemical and hormonal profile can be used as good indicator for semen value. Polymorphisms in HSP70 gene can affect semen quality. We suggest to be used in artificial insemination programs as marker assisted selection for anti-heat stress and good reproductive bulls.

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Section: Introductionmentioning

confidence: 99%

Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons

Gafer¹,

El-Rahman²,

Rawash³

2015

AJVS

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“…However, analogues of this gene have been reported in mammals and mice, also on chromosome 3 [21]. Despite the known effects of fluctuations of gene expression during extremely low temperatures, seasonal variations in expressions of genes on male fertility in high temperatures such as the report by [25] on expressions of Heat shock proteins (HSP) in Indian bulls has also shown the same outcome of reduced male fertility. Furthermore, translocation of genes could also have a compounding effect.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Bovine Spermatozoa CSP-Gene Expression using qPCR and Relative Quantification Method as Biodiagnostic Tool for Fertilizing Capacity

Ismail¹,

Mohamad²,

Jaafar³

et al. 2019

IJET

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Gene expression studies enable for real-time relative quantification of expressed genes. However, the incorporation of a primary discerning tool before amplification for specific sequences of genes of interest is yet to be implemented. The amplification of gene sequences from discriminate sample groups further enhances the results following qPCR and provides an absolute exclusive measurement for the defined sample. Cryopreserved spermatozoa have characteristics of compromised sperm quality as the cells are exposed to a rapid temperature downshift not normally encountered by such cells in vivo. Thus, the use of a separation system before cryopreservation produces a refined outcome in gene expression studies. The proliferation of Cold Shock Protein (CSP) can relatively be quantified by conducting qPCR following sperm selection and cryopreservation. CSPs are indicators of cells adapting to the decrease in temperature and indirectly acts as an intracellular protective mechanism for the cell. The current study compares the effects of sperm selection system in isolating a homogeneous population of spermatozoa for cryopreservation followed by quantification of CSP gene. Method of molecular assessment involved quantifying the amplified sequences via qPCR and gene expression following sperm isolation was significant in increasing spermatozoa viability by 26.2% (P< 0.05). The relative fold expression of CSP gene for treatment group increased from 1.00 fold to 1.38 fold. The Cq values for treatment group had recorded an earlier point of amplification (Cq= 24.87) as compared to control Cq= 25.83. Our findings suggest that the use of a sperm isolation system before cryopreservation would increase the probability of obtaining higher amplifications of CSP genes that would confer protection against extremely low temperatures during cryopreservation. This would increase the likelihood of in vitro fertilization using cryopreserved spermatozoa by implementing qPCR as a potential biomolecular diagnostic tool to ascertain the fertilizing potential of the spermatozoa.

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“…It was reported that buffalo bull seminal plasma contain specific proteins in particular season, which may be associated with some of the semen characteristics and fertility (Sharma et al, 2014). Studies also confirmed that expression of specific genes in spermatozoa varied between seasons (Rajoriya et al, 2014;Yang et al, 2010). Ejaculated sperm retain a complex, yet specific, population of RNAs, which may have important roles in sperm development, chromatin repackaging, zygote development and fertility (Miller et al, 2005).…”

Section: Alterations In Expression Of Sperm Molecules During Differentmentioning

confidence: 93%

Spermatozoa produced during winter are superior in terms of phenotypic characteristics and oviduct explants binding ability in the water buffalo (Bubalus bubalis)

Rather

Kumaresan

Nag

et al. 2020

Reprod Domestic Animals

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Although reduced reproductive efficiency during summer has been well documented in buffaloes, the reason for the same is yet to be understood. The present study was conducted to identify the subtle differences in sperm phenotypic characteristics (motility, membrane integrity, acrosome reaction and lipid peroxidation status), oviduct binding ability and expression of fertility-associated genes (AK 1, ATP5D, CatSper 1, Cytochrome P450 aromatase, SPP1 and PEBP1) between winter and summer seasons in buffaloes. Cryopreserved spermatozoa from 6 Murrah buffalo bulls (3 ejaculates/bull/season) were utilized for the study. Real-time quantitative PCR was performed for assessing the expression patterns of select fertility-associated genes. The proportion of motile and membrane intact spermatozoa was significantly higher (p < .05) in winter as compared to summer ejaculates. The proportion of moribund and lipid peroxidized spermatozoa was significantly lower (p < .05) in winter ejaculates as compared to summer. The sperm-oviduct binding index was significantly lower (p < .01) when spermatozoa from summer ejaculates were used as compared to winter ejaculates. The expression of fertility-associated genes did not differ significantly between the two seasons except for PEPB1; the transcriptional abundance of PEPB1 was significantly (p < .05) lower in summer as compared to winter season. It was inferred that buffalo spermatozoa produced during winter season were superior in terms of cryotolerance, membrane and acrosome integrity, lipid peroxidation status and the ability to bind with oviduct explants.

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Studies on effect of different seasons on expression of HSP70 and HSP90 gene in sperm of Tharparkar bull semen

Cited by 12 publications

References 25 publications

Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons

Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons

Analysis of Bovine Spermatozoa CSP-Gene Expression using qPCR and Relative Quantification Method as Biodiagnostic Tool for Fertilizing Capacity

Spermatozoa produced during winter are superior in terms of phenotypic characteristics and oviduct explants binding ability in the water buffalo (Bubalus bubalis)

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