Studies on collagen metabolism in rats

Heck, Th.; Hempel, Klaus; Lange, Heinrich; Romen, W

doi:10.1007/bf02912074

Cited by 9 publications

(2 citation statements)

References 9 publications

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“…Hence it is interest ing to note that in vivo incorporation exper iments revealed a diminished formation of liver and serum proteins as well as of colla gen type I under diabetic conditions [11,13,16,21,22,24,26,28], In contrast to these observations, most of the findings obtained by in vitro studies detected a raised GBM synthesis in diabetic metabolism [1,3,5,10,12], Isolated glomeruli of diabetic rats incor porate more specific GBM tracer than glo meruli of the controls.…”

Section: Discussioncontrasting

confidence: 47%

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Glomerular Basement Membrane Turnover in Young, Old, and Streptozotocin-Diabetic Rats

Romen¹,

Heck²,

Rauscher³

et al. 1980

Kidney Blood Press Res

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Diabetic and senile glomerulosclerosis consists of thickening of the glomerular basement membranes (GBM) and augmentation of the mesangial matrix. The pathogenesis of these alterations is still controversial. Therefore, an in vivo study of the metabolism and the chemical composition of the GBM in streptozotocin-diabetic and old rats reported here was conducted: The turnover rate of the GBM, determined by the incorporation of labeled hydroxyproline, is prolonged in preparations from diabetic and aging rats. This can be explained by the decreased synthesis and delayed degradation of the GBM material found in both experimental groups. The biochemical analyses of the GBM revealed a higher content of hydroxylated amino acids, especially OH-lys, in the old and diabetic animals. The findings suggest that diabetic and senile glomerulosclerosis results from a prolonged turnover of the GBM and GBM-like material under the altered metabolic conditions investigated in this study.

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Section: Discussioncontrasting

confidence: 47%

“…The data are published elsewhere [13,14], They indicate that the free 'H-pro is available to some extent in the plasma only for a short period of 1-2 h. In diabetes the availability time is even slightly diminished. The reutilization of 3H-pro is small, but detectable for weeks.…”

Section: Discussionmentioning

confidence: 94%

Glomerular Basement Membrane Turnover in Young, Old, and Streptozotocin-Diabetic Rats

Romen¹,

Heck²,

Rauscher³

et al. 1980

Kidney Blood Press Res

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Three-dimensional organization of fibroblasts and collagen fibrils in rat tail tendon

Squier

Bausch

1984

Cell Tissue Res.

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The orderly arrangement of fibroblasts and collagen in tendons and ligaments suggests that these cells may have precise relationships with one another and with the collagen fibrils. The spatial organization of rat tail tendon was therefore examined using scanning and transmission electron microscopy and by reconstructing a 35-microns long segment of tendon from serial transmission electron micrographs. Fibroblasts were regularly arranged in columns and showed more intimate association in the longitudinal than in the transverse plane. Thin cytoplasmic sheets extended up to 3 microns transversely, frequently forming junctional attachments with similar processes from adjacent cells or from the same cell. Longitudinal processes were longer, often extending for more than 20 microns and forming junctional attachments with other cells in the same column. Such processes often exhibited invaginations in which there were single fibrils or small groups of fibrils; this arrangement may be indicative of fibril elongation or may serve to transmit tension between the fibroblast and the collagen fibrils. This organization has interesting implications for the growth and function of other fibrous connective tissue, such as the periodontal ligament.

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Spatial relationships between fibroblasts during the growth of rat-tail tendon

Squier

Magnes

1983

Cell Tissue Res.

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Sections of tendons from the base of the tail of rats were taken at eight time intervals from 18 days in utero until 244 days after birth and were examined in the electron microscope. For each time period, measurements were made of the relative area of fibroblasts, collagen and interstitial material, of the number of fibroblasts per unit area of tendon and of the average area of individual fibroblasts. The spatial arrangement of fibroblasts in the tendon sections was described quantitatively using the "nearest neighbor" method. Initially there was a rapid increase in the area of collagen accompanied by a decrease in the area occupied by fibroblasts but after 104 days of age these values changed very little. The numbers of fibroblasts per unit area decreased steadily from the embryo until 104 days whereas the average size of each cell increased to reach a maximum area at 40 days of age and then declined. At all time intervals cells were arranged in a regular, dispersed pattern across the tendon fascicles. Growth in width of the rat tail appears to involve the secretion of collagen and other intercellular material symmetrically around each fibroblast, so as to gradually separate the cells until a stage is reached at which cells are sufficiently far apart that there is little contact between adjacent cell processes. This may interfere with the integration of metabolic activity in the tissue. As a consequence, there is shrinkage of the cell bodies and a reduction in secretory activity so that, between 55 and 104 days of age, the tendon enters a period of terminal senescence.

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Studies on collagen metabolism in rats

Cited by 9 publications

References 9 publications

Glomerular Basement Membrane Turnover in Young, Old, and Streptozotocin-Diabetic Rats

Glomerular Basement Membrane Turnover in Young, Old, and Streptozotocin-Diabetic Rats

Three-dimensional organization of fibroblasts and collagen fibrils in rat tail tendon

Spatial relationships between fibroblasts during the growth of rat-tail tendon

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