Studies on clostridium oedematiens

Roberts, RM; Güven, Serdar Can; Worrall, E.E.

doi:10.1016/0021-9975(70)90026-5

Cited by 7 publications

(5 citation statements)

References 6 publications

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“…The present study on DNA-DNA homology showed that C. novyi types A and B, C. haemolyticum, and C. botulinum type C strains were genetically divided into groups I (C. novyi type A), I1 (C. novyi type B, C. haemolyticum and one C. botulinum type C strain) and 111 (C. botulinurn type C). Smith & Hobbs (1974) found that C. haernolyticum and C. nouyi type B are separate species, although the major lethal toxin of C. haemolyticum is identical with the beta toxin of C. nouyi type B (Oakley & Warrack, 1959;Oakley et al, 1974) and the cultural properties of the two species resembled each other very closely (Holdeman & Moore, 1975;Nakamura et al, 1975 b ;Roberts et al, 1970;Rutter, 1970). The present study shows that C. haemolyticum and C. notlyi type B comprise one genetically homologous group (group 111).…”

Section: Discussionmentioning

confidence: 99%

“…haernol-vticum vary somewhat from one strain to another and also from one laboratory to another (Holdeman & Moore, 1975;Roberts et al, 1970;Rutter, 1970), Holdeman & Moore (1975) stated that mannose was fermented by most of C. novyi type B and C. haemolyticum strains but not by any of C. novyi type A strains, when the test was performed in the PRAS medium. Our results also confirmed this observation; none of group I strains (C. novyi type A) fermented mannose but all of group I1 strains did ferment this sugar.…”

Section: Discussionmentioning

confidence: 99%

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Taxonomic Relationships among Clostridium novyi Types A and B, Clostridium haemolyticum and Clostridium botulinum Type C

et al. 1983

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The present study was undertaken to examine the genetic relationships among the closely related species, Clostridium novyi types A and B, C. haemolyticum and C. botulinum type C. These species were tested for DNA-DNA homology and thermostability of DNA duplexes and sorted into three genetically related groups: I, C. novyi type A; II, C. novyi type B, C. haemolyticum and one C. botulinum type C strain (Stockholm); III, the remaining C. botulinum type C strains. A few biochemical criteria corresponding to the genetic differences were recommended to differentiate each group. These studies imply that C. haemolyticum might be considered as C. novyi type D and that there are two genetically different groups in C. botulinum type C.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Taxonomic Relationships among Clostridium novyi Types A and B, Clostridium haemolyticum and Clostridium botulinum Type C

et al. 1983

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“…The pathogenesis of C. hemolyticum is mediated by the beta toxin, a lethal hemolytic and necrotizing phospholipase type C serologically identical to the beta toxin of C. novyi type B [13,256]. However, C. hemolyticum produces more beta toxin than C. novyi type B [280,281]. The toxin provokes hepatocytes and erythrocytes destruction by hydrolysis of phosphatidylcholine into phosphocholine and diacylglyceride, as well as causing platelet aggregation and vascular permeability [270,277].…”

Section: Clostridium Hemolyticummentioning

confidence: 99%

Vaccine Production to Protect Animals Against Pathogenic Clostridia

Zaragoza

Orellana

Moonen³

et al. 2019

Toxins

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Clostridium is a broad genus of anaerobic, spore-forming, rod-shaped, Gram-positive bacteria that can be found in different environments all around the world. The genus includes human and animal pathogens that produce potent exotoxins that cause rapid and potentially fatal diseases responsible for countless human casualties and billion-dollar annual loss to the agricultural sector. Diseases include botulism, tetanus, enterotoxemia, gas gangrene, necrotic enteritis, pseudomembranous colitis, blackleg, and black disease, which are caused by pathogenic Clostridium. Due to their ability to sporulate, they cannot be eradicated from the environment. As such, immunization with toxoid or bacterin-toxoid vaccines is the only protective method against infection. Toxins recovered from Clostridium cultures are inactivated to form toxoids, which are then formulated into multivalent vaccines. This review discusses the toxins, diseases, and toxoid production processes of the most common pathogenic Clostridium species, including Clostridium botulinum, Clostridium tetani, Clostridium perfringens, Clostridium chauvoei, Clostridium septicum, Clostridium novyi and Clostridium hemolyticum.

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“…Toxin production media was prepared according to Roberts et al [42]. Two parts of 50 ml of the medium to which glucose was added at 1% were prepared to ensure anaerobic state and inoculated with 10 ml of 24 h -incubated cooked meat cultures of toxigenic isolates of C. perfringens ne part incu ated at C for 5-6 h for detection of C. perfringens types A, B and C and adjust pH every one hour at 7.5 and the other part incubated at C for 48 h for detection of type D and adjust pH at 7.5 twice daily, trypsin was added to the final concentration of to activate protoxin and incu ated at C for one hour.…”

Section: Typing Of C Perfringens Isolates By Dermonecrotic Testmentioning

confidence: 99%

Clostridium perfringens type A Causing Necrotic Enteritis Outbreaks among Chickens in Egypt

Helal

Khalaf

Menisy

et al. 2019

Zagazig Veterinary Journal

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Undoubtedly, necrotic enteritis is identified as one of the most threatening diseases which face poultry industry in Egypt and need radical solution to avoid huge economic losses. The present work was designed for typing Clostridium perfringens isolated from diseased chickens suspected to have necrotic enteritis from different outbreaks in six Governorates in Egypt (Gharbia, Dakhalia, Sharkia, Ismailia, North Sinai and Kafr El Sheikh) during the period from August to December 2018. Intestine and liver samples were obtained from eighty six diseased broiler chickens representing 60 flocks. C. perfringens was isolated and toxigenicity of the recovered isolates was determined by Nagler's and dermonecrotic reactions. Furthermore, multiplex polymerase chain reaction (PCR) targeting alpha, beta, epsilon and iota toxins' genes was performed for result confirmation and typing of the toxigenic isolates. Sixty-six C.perfringens isolates (38.37%) were recovered from 172 intestine and liver samples and twenty isolates (30.3%) were toxigenic and typed as C. perfringens type A producing alpha toxin only. These findings established the fact that alpha toxin is the only main toxin of C. perfringens type A which is basically responsible for its pathogenicity and virulence. In addition, most of the positively toxigenic isolates were isolated from hepatic lesions (15 isolates) rather than intestinal lesion (5 isolates). In conclusion, alpha toxin is a major toxin for NE development in chickens. Genotyping of Clostridium perfringens by multiplex PCR is a useful adjunct to diagnosis of necrotic enteritis in chickens.

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Studies on clostridium oedematiens

Cited by 7 publications

References 6 publications

Taxonomic Relationships among Clostridium novyi Types A and B, Clostridium haemolyticum and Clostridium botulinum Type C

Taxonomic Relationships among Clostridium novyi Types A and B, Clostridium haemolyticum and Clostridium botulinum Type C

Vaccine Production to Protect Animals Against Pathogenic Clostridia

Clostridium perfringens type A Causing Necrotic Enteritis Outbreaks among Chickens in Egypt

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