Studies on chemically induced dominant lethality. I. The cytogenetic basis of MMS-induced dominant lethality in post-meiotic male germ cells

Brewen, J.G.; Payne, H.S.; Jones, K. P.; Preston, R. Julian

doi:10.1016/0027-5107(75)90200-6

Cited by 103 publications

(17 citation statements)

References 6 publications

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“…Katoh 1979;Katoh and Tanaka 1980) in the germ cell stages, UDS was observed from spermatocytes to early and mid spermatids, except spermatozoa and late spermatids. The frequencies of MMS-induced chromosome aberrations decreased in early and mid spermatids showing UDS (Brewen et al 1975;Katoh and Tanaka 1980;Tanaka et al 1981). On the other hand, in the case of X-ray, the formation of chromosome aberrations in early and mid spermatids showing UDS indicated an increase than that in spermatozoa and late spermatids showing no UDS.…”

Section: Discussionmentioning

confidence: 85%

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X-ray-induced chromosome aberrations in postmeiotic stages of mice.

Katoh

Tanaka

Iwahara

1983

The Japanese journal of genetics

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Cytogenetic studies of spermatogenic radiosensitivity were performed by analysis of the first cleavage metaphase and by dominant lethal tests in mice. Male mice were subjected to acute X-irradiation (500 rad) and each male was then serially mated with two virgin females per week for six weeks. Chromosome aberrations were scored at the paternal chromosome sets in the first cleavage mataphases after fertilization. Spermatids were nearly twice as sensitive as spermatozoa to the induction of chromosome aberrations and dominant lethals, and chromosome-type aberrations were mainly induced. The profile of stage-specific chromosome aberrations induced by X-rays in male germ cells was different from that induced by chemical agents.

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Section: Discussionmentioning

confidence: 85%

“…It is well known that chemically induced dominant lethal mutations show stage-specific effects on mammalian spermatogenesis, and the principal cause is chromosome aberrations (Brewen et al 1975;Burki and Sheridan 1978;Katoh and Tanaka 1980;Tanaka et at. 1981;Katoh et at.…”

Section: Introductionmentioning

confidence: 99%

X-ray-induced chromosome aberrations in postmeiotic stages of mice.

Katoh

Tanaka

Iwahara

1983

The Japanese journal of genetics

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“…Treatment of mouse embryos with MMS at preimplantation stages was previously shown to induce their death both in vitro (Fabro et al, 1984;Iannaccone et al, 1987) and in vivo (Generoso et al, 1991;Rutledge et al, 1992;Nagao et al, 1997;Rutledge, 1997). Treatment of male rats or mice with MMS induces a dominant lethal phenotype in embryos d erived fro m th e ex pos ed sp er m (Ehling and Neuhauser-Klaus, 1990;Ashby et al, 1996) and chromosomal damage in gametes results in such a phenotype of embryos (Brewen et al, 1975;Katoh and Tanaka, 1980). Treatment of pregnant female mice with MMS at the zygote stage, however, induces fertilized eggs with degenerated chromosomes not to have aberrations at the first cleavage stage (Tanaka, 1981).…”

Section: Discussionmentioning

confidence: 99%

Differential Susceptibility of Rat Embryos to Methyl Methanesulfonate During the Pregastrulation Period

et al. 2007

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-The effects of exposure of pregnant rats to methyl methanesulfonate (MMS), an alkylating agent, during the pregastrulation period on embryonic and placental development were investigated. SD rats were treated orally with a single dose of MMS (200 mg/kg) in the morning of gestation days 0, 1, 2, 3, 4, 5, or 6 (GD0 to GD6 groups, respectively). The uterine contents including fetuses and placentas of the dams were examined on gestation day 20. The individual fetuses and placentas were weighed, and the fetuses were examined for external, visceral and skeletal anomalies. The progress of ossification was also evaluated. Both pre-and postimplantation embryonic mortalities were higher in the GD0 group than in the control group. The postimplantation loss was also increased for the GD3, GD4 and GD6 groups. Fetal malformations were rare in survivors of all the MMS-treated groups. Intrauterine growth retardation was apparent for fetuses in groups GD5 and GD6. In addition, placental weight was reduced in the GD6 group, but it was increased in the GD0 group. Effects of MMS on embryonic mortality or on fetal or placental growth were absent or minimal in the GD1 and GD2 groups. These results suggest that the susceptibility of rat embryos to MMS varies during the pregastrulation period.

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“…Damage induced in the genetic material of the developing male germ cell may not affect the ability ofresulting sperm to fertilize the ovum, yet can result in the death of the conceptus early in development. Experimental evidence has shown that the primary class of genetic damage responsible for dominant lethality is chromosomal aberrations and that the death of the conceptus results from an imbalance in the normal chromosomal complement following cell division (e.g., loss of chromosome fragments) (29). In addition to chromosomal aberrations, aneuploid gametes (having too few or too many chromosomes) may lead to dominant lethality and hence constitute a second class of genetic damage that is detected by this assay.…”

Section: Mouse Assays To Study Germ Cell Mutations Dominant Lethal Testmentioning

confidence: 99%

Fertility, reproduction, and genetic disease: studies on the mutagenic effects of environmental agents on mammalian germ cells.

Shelby

Bishop

Mason

et al. 1993

Environ Health Perspect

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Because geneticaly based diseases have a major inpact on human health, the National Institute of Environmental Health Sciences (NIEHS) has conducted a research and testing program for more than a decade to address chemical induction of heritable genetic damage in the germ cells of mammals. Although most genetic disease results from preexisting mutations, a portion is due to the occurrence ofnew mutations. The supposition that exposure to mutagenic c s contributes to the occurrence of new mutations in the human population is strongly supported by the results from animal models. Such studies clearly demonstrate the potential ofemnironmental chemicals to induce mutations in both somatic and reproductive cells of mammals. This NIEHS program has become a leader in the identification ofgenetic hazards in the environment and in the acquisition of animal model data used by regulatory agencies in assessing genetic risks to human health.

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Studies on chemically induced dominant lethality. I. The cytogenetic basis of MMS-induced dominant lethality in post-meiotic male germ cells

Cited by 103 publications

References 6 publications

X-ray-induced chromosome aberrations in postmeiotic stages of mice.

X-ray-induced chromosome aberrations in postmeiotic stages of mice.

Differential Susceptibility of Rat Embryos to Methyl Methanesulfonate During the Pregastrulation Period

Fertility, reproduction, and genetic disease: studies on the mutagenic effects of environmental agents on mammalian germ cells.

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