Studies on chemically induced dominant lethality III. Cytogenetic analysis of tem-effects on maturing dictyate mouse oocytes

Brewen, J.G.; Payne, H.S.

doi:10.1016/0027-5107(78)90063-5

Cited by 22 publications

(1 citation statement)

References 12 publications

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“…Apart from multigenerational studies and the classical dominant-lethal test (Bateman, 1958;Brewen and Payne, 1978), which does not discriminate between lethal mutations and aneuploidy, the direct analysis of numerical chromosomal abnormalities in sperm and oocytes of mice has been useful to detect aneugenic chemicals and study dose-responses (for review see: Adler et al, 2002;Eichenlaub-Ritter, 2003). The introduction of fluoresecent in situ hybridisation with chromosome-specific probes to detect aneuploidy has greatly aided the study of aneuploidy induction in mice, especially in the male (Sun et al, 2001;Adler et al, 2002;Scascitelli et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Mouse genetic models for aneuploidy induction in germ cells

Eichenlaub-Ritter¹

2005

Cytogenet Genome Res

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Rodents have been successfully used as models to identify risks of chemical exposures or age to aneuploidy induction in germ cells, which may be transmitted to the progeny. For this administration in vivo as well as exposures to in vitro maturing germ cells have been useful. Genetic models involving mice with structural chromosomal rearrangements and transgenic animals have the potential to model conditions predisposing to aneuploidy in one or both sexes, and in this way to identify potential targets for aneugens and gender-effects. The review provides an overview of mouse genetic models for aneuploidy induction in mammalian germ cells and discusses perspectives for combining genetic with experimental approaches in aneuploidy research.

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Section: Discussionmentioning

confidence: 99%

Mouse genetic models for aneuploidy induction in germ cells

Eichenlaub-Ritter¹

2005

Cytogenet Genome Res

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Induction and transmission of chromosome aberrations in mouse oocytes after treatment with butadiene diepoxide

Tiveron

Ranaldi

Bassani

et al. 1997

Environ. Mol. Mutagen.

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A study was conducted on the genotoxicity of butadiene diepoxide (DEB) in mouse oocytes. Superovulated female mice were injected intraperitoneally with DEB and mated with untreated males. Oocyte exposure occurred approximately 1.5 days before ovulation. DEB doses ranged between 26 and 52 mg/kg. Chromosome aberrations were scored in C‐banded metaphases of one‐cell embryos. The percentage of mated females, the average number of zygotes harvested per female, the frequencies of unfertilized oocytes and developmentally delayed zygotes did not reveal any overt sign of chemical toxicity which hindered the propensity of animals to mate or affected the ovulation, fertilization, or cell cycle progression of treated oocytes. A dose‐dependent induction of chromosome aberrations was observed which was best fitted by a linear quadratic equation. Half of all the aberrations transmitted by DEB‐treated oocytes were chromatid‐type breaks or exchanges. Among chromosome‐type aberrations, double fragments far exceeded chromosome exchanges. This spectrum of structural aberrations differed markedly from what was previously observed in one‐cell embryos conceived by DEB‐treated sperm, where 97% were chromosome‐type aberrations and 40% were dicentrics or translocations. This difference suggests that chromosome damage in one‐cell embryos can be fixed by different mutagenic pathways influenced by the targeted gamete and its specific chromatin configuration. After exposure to the same dose, oocytes transmitted to one‐cell embryos between 4 and 8 times fewer aberrations than DEB‐treated sperm. While the rate of aberration induction suggests that female germ cells may be less at risk than mature sperm,especially at low‐dose levels, the higher threshold for reproductive toxicity observed in female than in male mice may justify inclusion of data on female germ cell mutagenicity in the genetic risk assessment of butadiene exposure. Environ. Mol. Mutagen. 30:403–409, 1997 © 1997 Wiley‐Liss, Inc.

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Induction and transmission of chemically induced chromosome aberrations in female germ cells

Albanese¹

1987

Environ and Mol Mutagen

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Female mice were dosed with a number of chemical clastogens. The dosed females were then mated 28.5, 6.5, and 0.5 days after dosing, corresponding to various developmental stages of oogenesis (ie, early pre-antral to late antral stages). One-cell embryos derived from these mating were then isolated and analysed for structural and/or numerical chromosome aberrations. Chromosome damage, either structural or numerical, was induced only in oocytes at the late antral stages of oogenesis (i.e. when females were dosed 0.5 days prior to mating). There were few chromosome aberrations in embryos obtained from females dosed 6.5 or 28.5 days prior to mating. The results show that the analysis of one-cell embryos derived from the germ cells of dosed females is a potentially useful test system for the identification of chemically induced structural and numerical chromosome aberrations. However, the technically demanding methods involved in the preparation of these one-cell embryos prevent their use for routine screening purposes.

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Studies on chemically induced dominant lethality III. Cytogenetic analysis of tem-effects on maturing dictyate mouse oocytes

Cited by 22 publications

References 12 publications

Mouse genetic models for aneuploidy induction in germ cells

Mouse genetic models for aneuploidy induction in germ cells

Induction and transmission of chromosome aberrations in mouse oocytes after treatment with butadiene diepoxide

Induction and transmission of chemically induced chromosome aberrations in female germ cells

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