Studies on Chemical Composition of Pueraria lobata and Its Anti-Tumor Mechanism

Abstract

Fourteen compounds were isolated from Pueraria lobata (Willd.) Ohwi by column chromatography and preparative thin-layer chromatography; the structures were identified by spectroscopic analysis and compared with data reported in the literature. Seven compounds were isolated and identified from Pueraria lobata for the first time: Linoleic acid, Sandwicensin, Isovanillin, Ethyl ferulate, Haginin A, Isopterofuran, 3′.7-Dihydroxyisoflavan. The other 10 compounds were structurally identified as follows: Lupenone, Lupeol, β-sitosterol, Genistein, Medicarpin, Coniferyl Aldehyde, Syringaldehyde. All compounds were evaluated for their ability to inhibit SW480 and SW620 cells using the CCK-8 method; compound 5 (Sandwicensin) had the best activity, and compounds 6, 9, 11 and 12 exhibited moderate inhibitory activity. In addition, the targets and signaling pathways of Sandwicensin treatment for CRC were mined using network pharmacology, and MAPK3, MTOR, CCND1 and CDK4 were found to be closely associated with Sandwicensin treatment for CRC; the GO and KEGG analysis showed that Sandwicensin may directly regulate the cycle, proliferation and apoptosis of CRC cells through cancer-related pathways.

Ameliorative Effect of Medicarpin on Scopolamine-Induced Cognitive Impairment in Mice

“…MC is a pterocarpan, which is a derivative of isoflavonoids. In other studies, MC was isolated from Pueraia lobata [37], Medicago truncatula [38], Abrus precatorius [39], Butea monosperma [40], roots of Ononis angustissima L. [41], Robinia pseudoacacia L. [42], Radix hedysari [43], and other traditional Chinese herbs [44]. In addition, MC has been reported for various physiological activities such as anti-inflammatory [45], anti-cancer [46], and antioxidant [42].…”