Studies on cellulases of some cellulose-degrading soil fungi

Helal, G. A.; Khalil, Radwan; Galal, Y. G. M.; Soliman, S. M.; Elkader, Rofaida S. Abd

doi:10.1007/s00203-021-02705-9

Cited by 15 publications

(3 citation statements)

References 27 publications

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“…Endophytes were analysed for their antimicrobial activity by the plate method. Later, several exoenzymes produced by the endophytes, such as amylase (Alema et al 2020 , Elamary and Salem 2020 ), protease (Kalahroudi et al 2020 , Gong and Qi 2020 ), cellulase (Khosravi et al 2022 , Helal et al 2022 ), and chitinase (Saima et al 2013 , Aliabadi et al 2016 ) were analysed by both quantitative and qualitative methods.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula

Lodi

Dong

Jiang

et al. 2023

FEMS Microbiology Ecology

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The roots of the medicinal plant Codonopsis pilosula (Franch.) Nannf (C. pilosula) possess most medicinal supplements. In Current research on C. pilosula root endophytes were isolated, identified, and evaluated for their antimicrobial activity against human pathogens such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Pseudomonas aeruginosa and the fungi Candida albicans and Aspergillus niger. Endophytes C.P- 8 and C.P-20 exhibited very significant antimicrobial activity, the secondary metabolite of C.P-8 registered at retention time 24.075 by HPLC analysis. Significant minimum inhibitory concentration (MIC) of C.P-8 was exhibited at 250 µg/ml against Staphylococcus aureus and 500 µg/ml against Bacillus subtilis. Qualitative, quantitative analyses and partial purification of enzymes and purity was analysed by molecular weight determined by SDS‒PAGE of enzymes produced by C.P-20, amylase—64 kDa, protease—64 kDa, chitinase—30 kDa and cellulase—54 kDa. Optimum pH and temperature of the partially purified enzymes, was carried out. The partially purified enzymes from C.P-20 displayed maximum activity at pH 6–7 and temperatures of 40–45 °C. Moreover, the above endophytes will be useful tools for producing active enzymes and active bio-antimicrobial agents against human pathogens.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula

Lodi

Dong

Jiang

et al. 2023

FEMS Microbiology Ecology

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“…The cellulose component of plant cell walls and vegetable stalks, for example, can be completely degraded by fungi. A cellulase enzyme isolated and purified from Rhizopus oryzae has been reported to be useful for the enzymatic digestion, dissolution, and hydrolysis of cellulose materials [ 144 ]. Bacteria from the phyla Actinobacteria [ 145 , 146 ], Proteobacteria [ 147 ], and Bacillota also produce many cellulases [ 148 ].…”

Section: Lignocellulosic Biorefineries and The Roles Of Lignocellulol...mentioning

confidence: 99%

Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy

Asemoloye,

Bello,

Oladoye

et al. 2023

Bioengineered

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The next milestone of synthetic biology research relies on the development of customized microbes for specific industrial purposes. Metabolic pathways of an organism, for example, depict its chemical repertoire and its genetic makeup. If genes controlling such pathways can be identified, scientists can decide to enhance or rewrite them for different purposes depending on the organism and the desired metabolites. The lignocellulosic biorefinery has achieved good progress over the past few years with potential impact on global bioeconomy. This principle aims to produce different bio-based products like biochemical(s) or biofuel(s) from plant biomass under microbial actions. Meanwhile, yeasts have proven very useful for different biotechnological applications. Hence, their potentials in genetic/metabolic engineering can be fully explored for lignocellulosic biorefineries. For instance, the secretion of enzymes above the natural limit (aided by genetic engineering) would speed-up the down-line processes in lignocellulosic biorefineries and the cost. Thus, the next milestone would greatly require the development of synthetic yeasts with much more efficient metabolic capacities to achieve basic requirements for particular biorefinery. This review gave comprehensive overview of lignocellulosic biomaterials and their importance in bioeconomy. Many researchers have demonstrated the engineering of several ligninolytic enzymes in heterologous yeast hosts. However, there are still many factors needing to be well understood like the secretion time, titter value, thermal stability, pH tolerance, and reactivity of the recombinant enzymes. Here, we give a detailed account of the potentials of engineered yeasts being discussed, as well as the constraints associated with their development and applications.

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“…The enzymatic activity responsible for degrading the cell wall of plants by the pathogen was assessed using the 3,5-dinitrosalicylic acid method [26], specifically measuring the activities of cellulase (CX), β-glucosidase (βG), pectin methylgalactuionase (PMG), polygalacturonase (PG), and xylanase.…”

Section: Plant-cell-wall-degrading Enzyme (Cwde) Activity Of Pathogenmentioning

confidence: 99%

A Study of Soil-Borne Fusarium Wilt in Continuous Cropping Chrysanthemum Cultivar ‘Guangyu’ in Henan, China

Liu,

Jin,

Chen

et al. 2023

JoF

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Cut chrysanthemum, known as a highly favored floral choice globally, experiences a significant decline in production due to continuous cropping. The adverse physiological effects on cut chrysanthemums result from the degradation of a soil’s physical and chemical properties, coupled with the proliferation of pathogens. The “Guangyu” cultivar in Xinxiang, Henan Province, China, has been specifically influenced by these effects. First, the precise pathogen accountable for wilt disease was effectively identified and validated in this study. An analysis was then conducted to examine the invasion pattern of the pathogen and the physiological response of chrysanthemum. Finally, the PacBio platform was employed to investigate the dynamic alterations in the microbial community within the soil rhizosphere by comparing the effects of 7 years of monocropping with the first year. Findings indicated that Fusarium solani was the primary causative agent responsible for wilt disease, because it possesses the ability to invade and establish colonies in plant roots, leading to alterations in various physiological parameters of plants. Continuous cropping significantly disturbed the microbial community composition, potentially acting as an additional influential factor in the advancement of wilt.

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Studies on cellulases of some cellulose-degrading soil fungi

Cited by 15 publications

References 27 publications

Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula

Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula

Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy

A Study of Soil-Borne Fusarium Wilt in Continuous Cropping Chrysanthemum Cultivar ‘Guangyu’ in Henan, China

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